Page last updated: 2024-12-06

5-chloro-2-methyl-4-isothiazolin-3-one

Description Research Excerpts Clinical Trials Roles Classes Pathways Study Profile Bioassays Occurs in Manufacturing Related Drugs Related Conditions Protein Interactions Research Growth Market Indicators

Description

5-chloro-2-methyl-4-isothiazolin-3-one (CMIT) is a broad-spectrum biocide with antimicrobial activity against bacteria, fungi, and algae. It is commonly used as a preservative in a wide range of consumer products, including paints, cosmetics, and personal care products. CMIT is synthesized through a multi-step process involving the reaction of chloroacetaldehyde with methyl mercaptan and sulfuryl chloride. The compound exhibits its antimicrobial effects by interfering with the cell membrane of microorganisms, leading to cell death. The effectiveness of CMIT as a preservative is attributed to its ability to penetrate cell membranes and disrupt vital cellular processes. However, concerns have been raised regarding its potential toxicity and allergenic properties. CMIT has been linked to skin irritation, allergic reactions, and respiratory problems. Studies have been conducted to evaluate its environmental impact, bioaccumulation potential, and long-term health effects. The understanding of CMIT's synthesis, effects, and potential risks is crucial for its responsible use and the development of safer alternatives.'
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5-chloro-2-methyl-4-isothiazolin-3-one: RN given refers to parent cpd; structure [Medical Subject Headings (MeSH), National Library of Medicine, extracted Dec-2023]

chloromethylisothiazolinone : A 1,2-thiazole that is 4-isothiazolin-3-one bearing a methyl group on the nitrogen atom and a chlorine at C-5. It is a powerful biocide and preservative and is the major active ingredient in the commercial product Kathon(TM). [Chemical Entities of Biological Interest (ChEBI), Hastings J, Owen G, Dekker A, Ennis M, Kale N, Muthukrishnan V, Turner S, Swainston N, Mendes P, Steinbeck C. (2016). ChEBI in 2016: Improved services and an expanding collection of metabolites. Nucleic Acids Res]

Cross-References

ID SourceID
PubMed CID33344
CHEMBL ID1738962
CHEBI ID53621
SCHEMBL ID111860
SCHEMBL ID20686
MeSH IDM0057724

Synonyms (81)

Synonym
5-chloro-2-methyl-3(2h)-isothiazolone
5-chloro-2-methyl-4-isothiazolin-3-one
5-chloro-2-methylisothiazolin-3-one
4-isothiazolin-3-one, 5-chloro-2-methyl-
3(2h)-isothiazolone, 5-chloro-2-methyl-
brn 1210149
einecs 247-500-7
5-chloro-n-methylisothiazolone
kathon cg 5243
methylchloroisothiazolinone
5-chloro-2-methyl-2h-isothiazol-3-one
NCGC00181041-01
5-chloro-2-methyl-1,2-thiazol-3-one
2,3-dihydro-2-methyl-3-oxo-5-chloroisothiazole
cmit
5-chloro-2-methyl-1,2-thiazol-3(2h)-one
26172-55-4
chloromethylisothiazolinone
CHEBI:53621 ,
5-chloro-2-methylisothiazol-3(2h)-one
NCGC00181041-02
5-chloro-2-methyl-4-isothiazoline-3-one
cas-26172-55-4
NCGC00254127-01
dtxsid9034286 ,
dtxcid7014286
tox21_112689
tox21_300199
AKOS006230760
AM806586
hsdb 8270
bioace
hs 818 (antiseptic)
t 360
5-chloro-2-methyl-2h-isothiazolin-3-one
unii-del7t5qrpn
hs 818
5-chloro-2-methyl-3(2h)-isothiazolinone
kathon ixe
a 33 (bactericide)
del7t5qrpn ,
n-methyl-5-chloroisothiazolone
5-chloro-2-methyl-3-isothiazolone
5-chloro-n-methylisothiazolin-3-one
n-methyl-5-chloroisothiazolin-3-one
2-methyl-5-chloro-3-isothiazolone
2-methyl-5-chloroisothiazolin-3-one
5-chloro-2-methyl-3-isothiazolone-d3
FT-0620267
hs-818
5-chloro-2-methyl-isothiazol-3-one
methylchloroisothiazolinone [vandf]
methylchloroisothiazolinone [inci]
methylchloroisothiazolinone [mart.]
5-chlor-2-methyl-4-isothiazolin-3-one
methylchloroisothiazolinone [mi]
methylchloroisothiazolinone [ii]
methylchloroisothiazolinone [who-dd]
5-chloro-2-methyl-isothiazol-3(2h)-one
SCHEMBL111860
SCHEMBL20686
W-107193
CHEMBL1738962
mfcd00792550
5-chloro-2-methyl-2,3-dihydro-1,2-thiazol-3-one
GS-3223
sr-01000944864
SR-01000944864-1
5-chloro-2-methyl-4-isothiazolin-3-one (active ingredient >14%, cmi/mi 2.5 - 4.0)
CS-W022348
DB14197
5-chloro-2-methyl-4-isothiazolin-3-one, tech grade, >14% in water. cmi/mi >2.0
5-chloro-2-methyl-4-isothiazolin-3-one 100 microg/ml in acetonitrile
Q204121
n-methyl-5-chloroisothiazolone (5-chloro-2-methyl-4-isothiazolin-3-one)
H10371
5-chloro-2-methyl-4-isothiazolin-3-one d3 (methyl d3)
5-chloro-2-methyl-3-isothiazolone (~90%)
EN300-7409393
5-chloro-2-methyl-4-isothiazolin-3-one (cmi/mi > 2.0)
Z2417817741

Research Excerpts

Toxicity

ExcerptReferenceRelevance
" We therefore suggest that a systemic toxicity approach should be considered to comprehensively understand the adverse health effects of humidifier disinfectant misuse."( Early life exposure of a biocide, CMIT/MIT causes metabolic toxicity via the O-GlcNAc transferase pathway in the nematode C. elegans.
Choi, J; Kim, Y, 2019
)
0.51

Bioavailability

ExcerptReferenceRelevance
"The ATP-binding cassette transporter P-glycoprotein (P-gp) is known to limit both brain penetration and oral bioavailability of many chemotherapy drugs."( A High-Throughput Screen of a Library of Therapeutics Identifies Cytotoxic Substrates of P-glycoprotein.
Ambudkar, SV; Brimacombe, KR; Chen, L; Gottesman, MM; Guha, R; Hall, MD; Klumpp-Thomas, C; Lee, OW; Lee, TD; Lusvarghi, S; Robey, RW; Shen, M; Tebase, BG, 2019
)
0.51

Dosage Studied

ExcerptRelevanceReference
" The study was designed as a double-blind, placebo-controlled, dose-response ROAT preceded by a diagnostic patch testing."( An evaluation of dose/unit area and time as key factors influencing the elicitation capacity of methylchloroisothiazolinone/methylisothiazolinone (MCI/MI) in MCI/MI-allergic patients.
Gray, JE; Lerbaek, A; McNamee, PM; Menné, T; Wooder, M; Zachariae, C, 2006
)
0.33
" The elicitation doses were calculated, and fitted dose-response curves were drawn."( Can exposure limitations for well-known contact allergens be simplified? An analysis of dose-response patch test data.
Fischer, LA; Johansen, JD; Menné, T; Voelund, A, 2011
)
0.37
" At the end of the dosing period, organs were weighed and histological examinations performed."( Effects of a 28-day oral exposure to a 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one biocide formulation in Sprague-Dawley rats.
Pelletier, G; Poon, R; Rigden, M; Valli, VE, 2014
)
0.67
" We have examined the human metabolism of methylisothiazolinone and chloromethylisothiazolinone after oral dosage of stable isotope-labelled analogues."( Urinary excretion kinetics of the metabolite N-methylmalonamic acid (NMMA) after oral dosage of chloromethylisothiazolinone and methylisothiazolinone in human volunteers.
Kraus, T; Schettgen, T, 2017
)
0.46
" We have previously examined the human metabolism of MI and MCI after oral dosage of isotope-labelled analogues in human volunteers and confirmed N-methylmalonamic acid to be a major, but presumably unspecific human urinary metabolite."( New data on the metabolism of chloromethylisothiazolinone and methylisothiazolinone in human volunteers after oral dosage: excretion kinetics of a urinary mercapturic acid metabolite ("M-12").
Bertram, J; Kraus, T; Schettgen, T, 2021
)
0.62
[information is derived through text-mining from research data collected from National Library of Medicine (NLM), extracted Dec-2023]

Occurs in Manufacturing (27 Product(s))

Product Categories

Product CategoryProducts
Household Essentials22
Beauty & Personal Care5

Products

ProductBrandCategoryCompounds Matched from IngredientsDate Retrieved
Australian Gold Foaming Hand Soap Aloe Vera -- 7.5 fl ozAustralian GoldHousehold Essentialscitric acid, methylchloroisothiazolinone, benzyl alcohol, benzyl salicylate, citric acid, coumarin, coumarin, sodium laureth sulfate, glycerin, hexyl cinnamal, linalool, magnesium nitrate, propylene glycol, coco-betaine, sodium bicarbonate, sodium sulfate, triethylene glycol2024-11-29 10:47:42
Australian Gold Foaming Hand Soap Cocoa Dreams -- 7.5 fl ozAustralian GoldHousehold Essentialscitric acid, methylchloroisothiazolinone, benzyl alcohol, benzyl benzoate, citral, citric acid, coumarin, coumarin, sodium laureth sulfate, glycerin, limonene, linalool, magnesium nitrate, propylene glycol, coco-betaine, sodium benzoate, sodium bicarbonate, sodium sulfate, triethylene glycol2024-11-29 10:47:42
Australian Gold Foaming Hand Soap Lavender Mint -- 7.5 fl ozAustralian GoldHousehold Essentialscitric acid, methylchloroisothiazolinone, benzyl alcohol, citric acid, sodium laureth sulfate, glycerin, linalool, magnesium nitrate, propylene glycol, coco-betaine, sodium benzoate, sodium bicarbonate, sodium sulfate, triethylene glycol2024-11-29 10:47:42
Australian Gold Foaming Hand Soap Sugared Lemon -- 7.5 fl ozAustralian GoldHousehold Essentialscitric acid, methylchloroisothiazolinone, alphaisomethyl ionone, benzyl alcohol, citral, citric acid, sodium laureth sulfate, glycerin, linalool, magnesium nitrate, propylene glycol, coco-betaine, sodium benzoate, sodium bicarbonate, sodium sulfate, triethylene glycol2024-11-29 10:47:42
Australian Gold Foaming Hand Soap Sweet Apricot -- 7.5 fl ozAustralian GoldHousehold Essentialscitric acid, methylchloroisothiazolinone, benzyl alcohol, benzyl benzoate, citric acid, sodium laureth sulfate, geraniol, glycerin, hexyl cinnamal, limonene, linalool, magnesium nitrate, propylene glycol, coco-betaine, sodium benzoate, sodium bicarbonate, sodium sulfate, triethylene glycol2024-11-29 10:47:42
Australian Gold Liquid Hand Soap Aloe Vera -- 11.5 fl ozAustralian GoldHousehold Essentialscitric acid, methylchloroisothiazolinone, benzyl alcohol, benzyl salicylate, citric acid, cocamidopropyl betaine, sodium laureth sulfate, disodium EDTA, glycerin, hexyl cinnamal, linalool, magnesium nitrate, propylene glycol, sodium bicarbonate, sodium sulfate, triethylene glycol2024-11-29 10:47:42
Australian Gold Liquid Hand Soap Cocoa Dreams -- 11.5 fl ozAustralian GoldHousehold Essentialscitric acid, methylchloroisothiazolinone, benzyl alcohol, citral, citric acid, cocamidopropyl betaine, coumarin, coumarin, sodium laureth sulfate, disodium EDTA, glycerin, limonene, linalool, magnesium nitrate, propylene glycol, sodium benzoate, sodium bicarbonate, sodium sulfate, triethylene glycol2024-11-29 10:47:42
Australian Gold Liquid Hand Soap Lavender Mint -- 11.5 fl ozAustralian GoldHousehold Essentialscitric acid, methylchloroisothiazolinone, benzyl alcohol, citric acid, cocamidopropyl betaine, sodium laureth sulfate, disodium EDTA, glycerin, limonene, linalool, magnesium nitrate, propylene glycol, sodium benzoate, sodium bicarbonate, sodium sulfate, triethylene glycol2024-11-29 10:47:42
Australian Gold Liquid Hand Soap Sugared Lemon -- 11.5 fl ozAustralian GoldHousehold Essentialscitric acid, methylchloroisothiazolinone, alpha-isomethyl ionone, benzyl alcohol, citral, citric acid, cocamidopropyl betaine, sodium laureth sulfate, disodium EDTA, glycerin, limonene, linalool, magnesium nitrate, propylene glycol, sodium benzoate, sodium bicarbonate, sodium sulfate, triethylene glycol2024-11-29 10:47:42
Australian Gold Liquid Hand Soap Sweet Apricot -- 11.5 fl ozAustralian GoldHousehold Essentialscitric acid, methylchloroisothiazolinone, benzyl alcohol, benzyl benzoate, citral, citric acid, citronellol, cocamidopropyl betaine, sodium laureth sulfate, disodium EDTA, geraniol, glycerin, hexyl cinnamal, limonene, linalool, magnesium nitrate, propylene glycol, sodium benzoate, sodium bicarbonate, sodium sulfate, triethylene glycol2024-11-29 10:47:42
Daeng Gi Meo Ri Shampoo For Damaged Hair -- 16.9 fl ozDaeng Gi Meo RiBeauty & Personal Caremethylchloroisothiazolinone, allantoin, carbomer, cocamidopropyl betaine, panthenol, lauramine oxide, disodium EDTA, glycerin, dimethicone, menthol, methylparaben, sodium cocoyl glutamate, triethanolamine2024-11-29 10:47:42
Formula 10.0.6 Go Get Fresh Deodorizing Body Wash -- 10.1 fl ozFormula 10.0.6Beauty & Personal Caremethylchloroisothiazolinone, cocamidopropyl betaine, disodium EDTA, glycerin, propylene glycol, sorbic acid2024-11-29 10:47:42
Formula 10.0.6 One Smooth Operator Facial Scrub -- 3.4 fl ozFormula 10.0.6Beauty & Personal Caremethylisothiazolinone, methylchloroisothiazolinone, carbomer, glycerin, magnesium sulfate, oat, sodium hydroxide2024-11-29 10:47:42
Love Beauty and Planet Sulfate-Free Murumuru Butter & Rose Color-Treated Hair Vegan Conditioner -- 13.5 fl ozLove Beauty and PlanetBeauty & Personal Caremethylisothiazolinone, methylchloroisothiazolinone, benzyl salicylate, disodium EDTA2024-11-29 10:47:42
Method Dish Soap Refill Clementine -- 54 fl ozMethodHousehold Essentialsmethylisothiazolinone, citric acid, methylchloroisothiazolinone, citric acid, decyl glucoside, lauramine oxide, glycerin, limonene, linalool, sodium lauryl sulfate2024-11-29 10:47:42
Method Foaming Hand Wash Lemon Mint -- 10 fl ozMethodHousehold Essentialsmethylisothiazolinone, citric acid, methylchloroisothiazolinone, citric acid, cocamidopropyl betaine, vitamin E, vitamin E, glycerin, limonene, sodium citrate, sodium lauryl sulfate, benzophenone-4, yellow 52024-11-29 10:47:42
Method Foaming Hand Wash Refill Coconut Water -- 28 fl ozMethodHousehold Essentialsmethylisothiazolinone, methylchloroisothiazolinone, vitamin E, vitamin E2024-11-29 10:47:42
Method Foaming Hand Wash Refill French Lavender -- 28 fl ozMethodHousehold Essentialsmethylisothiazolinone, methylchloroisothiazolinone, vitamin E, vitamin E, linalool2024-11-29 10:47:42
Method Foaming Hand Wash Refill Pink Grapefruit -- 28 fl ozMethodHousehold Essentialsmethylisothiazolinone, methylchloroisothiazolinone, vitamin E, red 33, vitamin E, hexyl cinnamic aldehyde, limonene, linalool2024-11-29 10:47:42
Method Gel Hand Wash Pink Grapefruit -- 12 fl ozMethodHousehold Essentialsmethylisothiazolinone, citric acid, methylchloroisothiazolinone, citric acid, cocamidopropyl betaine, vitamin E, red 33, vitamin E, glycerin, sodium citrate, sodium lauryl sulfate, benzophenone-42024-11-29 10:47:42
Method Gel Hand Wash Refill - French Lavender -- 34 fl ozMethodHousehold Essentialsmethylisothiazolinone, methylchloroisothiazolinone, vitamin e, vitamin e, linalool2024-11-29 10:47:42
Method Gel Hand Wash Refill - Pink Grapefruit -- 34 fl ozMethodHousehold Essentialsmethylisothiazolinone, methylchloroisothiazolinone, vitamin E, red 33, vitamin E, hexyl cinnamic aldehyde, limonene, linalool2024-11-29 10:47:42
Method Gel Hand Wash Refill - Sea Minerals -- 34 fl ozMethodHousehold Essentialsmethylisothiazolinone, methylchloroisothiazolinone, vitamin E, vitamin E, hexyl cinnamal, linalool2024-11-29 10:47:42
Method Gel Hand Wash Refill - Sweet Water -- 34 fl ozMethodHousehold Essentialsmethylisothiazolinone, methylchloroisothiazolinone, benzyl salicylate, vitamin E, vitamin E, butylphenyl methylpropional2024-11-29 10:47:42
Method Gel Hand Wash Rosemary -- 11.5 fl ozMethodHousehold Essentialsmethylisothiazolinone, methylchloroisothiazolinone, vitamin E, vitamin E2024-11-29 10:47:42
Method Gel Hand Wash Sweet Water -- 12 fl ozMethodHousehold Essentialscitric acid, methylchloroisothiazolinone, tocopherol acetate, citric acid, cocamidopropyl betaine, glycerin, sodium citrate, sodium lauryl sulfate, benzophenone-42024-11-29 10:47:42
Miracle Anti-Aging™ Shampoo -- 6 fl ozMiracleBeauty & Personal Carephytantriol, citric acid, methylchloroisothiazolinone, ascorbyl palmitate, vitamin E acetate, citric acid, cocamidopropyl betaine, panthenol, pro-vitamin B5, glycerin, linoleic acid, menthol, retinyl palmitate2024-11-29 10:47:42

Roles (3)

RoleDescription
antimicrobial agentA substance that kills or slows the growth of microorganisms, including bacteria, viruses, fungi and protozoans.
xenobioticA xenobiotic (Greek, xenos "foreign"; bios "life") is a compound that is foreign to a living organism. Principal xenobiotics include: drugs, carcinogens and various compounds that have been introduced into the environment by artificial means.
environmental contaminantAny minor or unwanted substance introduced into the environment that can have undesired effects.
[role information is derived from Chemical Entities of Biological Interest (ChEBI), Hastings J, Owen G, Dekker A, Ennis M, Kale N, Muthukrishnan V, Turner S, Swainston N, Mendes P, Steinbeck C. (2016). ChEBI in 2016: Improved services and an expanding collection of metabolites. Nucleic Acids Res]

Drug Classes (2)

ClassDescription
1,2-thiazoles
organochlorine compoundAn organochlorine compound is a compound containing at least one carbon-chlorine bond.
[compound class information is derived from Chemical Entities of Biological Interest (ChEBI), Hastings J, Owen G, Dekker A, Ennis M, Kale N, Muthukrishnan V, Turner S, Swainston N, Mendes P, Steinbeck C. (2016). ChEBI in 2016: Improved services and an expanding collection of metabolites. Nucleic Acids Res]

Protein Targets (9)

Potency Measurements

ProteinTaxonomyMeasurementAverage (µ)Min (ref.)Avg (ref.)Max (ref.)Bioassay(s)
RAR-related orphan receptor gammaMus musculus (house mouse)Potency51.52020.006038.004119,952.5996AID1159521; AID1159523
AR proteinHomo sapiens (human)Potency19.38090.000221.22318,912.5098AID743040; AID743042; AID743054
glucocorticoid receptor [Homo sapiens]Homo sapiens (human)Potency23.63860.000214.376460.0339AID720691; AID720719
retinoic acid nuclear receptor alpha variant 1Homo sapiens (human)Potency64.75280.003041.611522,387.1992AID1159555
estrogen nuclear receptor alphaHomo sapiens (human)Potency0.15480.000229.305416,493.5996AID743075
cytochrome P450, family 19, subfamily A, polypeptide 1, isoform CRA_aHomo sapiens (human)Potency29.84930.001723.839378.1014AID743083
v-jun sarcoma virus 17 oncogene homolog (avian)Homo sapiens (human)Potency51.03190.057821.109761.2679AID1159526; AID1159528
Histone H2A.xCricetulus griseus (Chinese hamster)Potency126.79300.039147.5451146.8240AID1224845
thyroid hormone receptor beta isoform 2Rattus norvegicus (Norway rat)Potency52.20380.000323.4451159.6830AID743065; AID743067
[prepared from compound, protein, and bioassay information from National Library of Medicine (NLM), extracted Dec-2023]

Bioassays (4)

Assay IDTitleYearJournalArticle
AID1296008Cytotoxic Profiling of Annotated Libraries Using Quantitative High-Throughput Screening2020SLAS discovery : advancing life sciences R & D, 01, Volume: 25, Issue:1
Cytotoxic Profiling of Annotated and Diverse Chemical Libraries Using Quantitative High-Throughput Screening.
AID1346986P-glycoprotein substrates identified in KB-3-1 adenocarcinoma cell line, qHTS therapeutic library screen2019Molecular pharmacology, 11, Volume: 96, Issue:5
A High-Throughput Screen of a Library of Therapeutics Identifies Cytotoxic Substrates of P-glycoprotein.
AID1346987P-glycoprotein substrates identified in KB-8-5-11 adenocarcinoma cell line, qHTS therapeutic library screen2019Molecular pharmacology, 11, Volume: 96, Issue:5
A High-Throughput Screen of a Library of Therapeutics Identifies Cytotoxic Substrates of P-glycoprotein.
AID504749qHTS profiling for inhibitors of Plasmodium falciparum proliferation2011Science (New York, N.Y.), Aug-05, Volume: 333, Issue:6043
Chemical genomic profiling for antimalarial therapies, response signatures, and molecular targets.
[information is prepared from bioassay data collected from National Library of Medicine (NLM), extracted Dec-2023]

Research

Studies (230)

TimeframeStudies, This Drug (%)All Drugs %
pre-19907 (3.04)18.7374
1990's37 (16.09)18.2507
2000's33 (14.35)29.6817
2010's126 (54.78)24.3611
2020's27 (11.74)2.80
[information is prepared from research data collected from National Library of Medicine (NLM), extracted Dec-2023]

Market Indicators

Research Demand Index: 41.36

According to the monthly volume, diversity, and competition of internet searches for this compound, as well the volume and growth of publications, there is estimated to be strong demand-to-supply ratio for research on this compound.

MetricThis Compound (vs All)
Research Demand Index41.36 (24.57)
Research Supply Index5.60 (2.92)
Research Growth Index5.43 (4.65)
Search Engine Demand Index61.29 (26.88)
Search Engine Supply Index2.00 (0.95)

This Compound (41.36)

All Compounds (24.57)

Study Types

Publication TypeThis drug (%)All Drugs (%)
Trials4 (1.50%)5.53%
Reviews17 (6.39%)6.00%
Case Studies75 (28.20%)4.05%
Observational1 (0.38%)0.25%
Other169 (63.53%)84.16%
[information is prepared from research data collected from National Library of Medicine (NLM), extracted Dec-2023]