Page last updated: 2024-12-07

dansyl hydrazine

Description Research Excerpts Clinical Trials Roles Classes Pathways Study Profile Bioassays Related Drugs Related Conditions Protein Interactions Research Growth Market Indicators

Dansyl hydrazine (DNSH) is a fluorescent reagent widely used in biochemical research. It is a derivative of dansyl chloride, which is a sulfonyl chloride that reacts with primary amines to form highly fluorescent dansyl derivatives. DNSH is particularly useful for labeling aldehydes and ketones due to its reactivity with carbonyl groups. The reaction of DNSH with aldehydes or ketones produces a stable hydrazone derivative, which can then be detected and quantified using fluorescence spectroscopy. DNSH labeling is often used in studies of protein modification, enzyme kinetics, and the analysis of biological samples. The reaction of DNSH with carbonyl groups is relatively specific and can be performed under mild conditions, making it a valuable tool for studying a variety of biological processes.'

Cross-References

ID SourceID
PubMed CID94442
SCHEMBL ID235824
MeSH IDM0090312

Synonyms (34)

Synonym
5-(dimethylamino)naphthalene-1-sulfonohydrazide
OPREA1_436969
SR-01000633636-1
dansylhydrazine, 98%
5-dimethylaminonaphthalene-1-sulfonyl hydrazine
33008-06-9
dansyl hydrazine
dansylhydrazine
bdbm36661
1-naphthalenesulfonic acid, 5-(dimethylamino)-hydrazide
einecs 251-337-7
5-dimethylaminonaphthalene-1-sulphonohydrazide
CCG-43740
FT-0624414
FT-0624422
AKOS015894725
5-(dimethylamino)-1-naphthalenesulfonic hydrazide
SCHEMBL235824
1-naphthalenesulfonic acid, 5-(dimethylamino)-, hydrazide
5-(dimethylamino)-1-naphthalenesulfonohydrazide #
KPQYDVAFRDWIBW-UHFFFAOYSA-N
1-dimethylaminophthalene-5-sulphonyl hydrazine
mfcd00003986
DTXSID90186640
dansylhydrazine, bioreagent, suitable for fluorescence, >=90% (hplc)
dansylhydrazine, for lc-ms derivatization, >=95% (hplc)
J-018973
5-(dimethylamino)-1-naphthalenesulfonohydrazide
T70785
dansyl hydrazine [for hplc labeling]
AS-56735
CS-W010127
SY050086
HY-W009411

Research Excerpts

Pharmacokinetics

ExcerptReferenceRelevance
" Tacrolimus demonstrates considerable interindividual variation in its pharmacokinetic profile."( Measurement of tacrolimus (FK506) and its metabolites: a review of assay development and application in therapeutic drug monitoring and pharmacokinetic studies.
Alak, AM, 1997
)
0.3
" Due to its simplicity and sensitivity this method can be used on a routine basis for pharmacokinetic analysis of neuroactive steroids."( High-performance liquid chromatography of the neuroactive steroids alphaxalone and pregnanolone in plasma using dansyl hydrazine as fluorescent label: application to a pharmacokinetic-pharmacodynamic study in rats.
Danhof, M; Gladdines, WW; Irth, H; Smulders, CJ; van der Graaf, PH; Visser, SA, 2000
)
0.52
[information is derived through text-mining from research data collected from National Library of Medicine (NLM), extracted Dec-2023]

Protein Targets (3)

Inhibition Measurements

ProteinTaxonomyMeasurementAverageMin (ref.)Avg (ref.)Max (ref.)Bioassay(s)
Thymidylate synthaseLacticaseibacillus caseiIC50 (µMol)511.75000.00901.93248.8000AID1799451; AID1799474
Thymidylate synthaseHomo sapiens (human)IC50 (µMol)536.00000.00662.06379.5000AID1799474
Thymidylate synthaseEscherichia coli K-12IC50 (µMol)536.00000.00660.95886.8000AID1799474
[prepared from compound, protein, and bioassay information from National Library of Medicine (NLM), extracted Dec-2023]

Biological Processes (24)

Processvia Protein(s)Taxonomy
dTMP biosynthetic processThymidylate synthaseHomo sapiens (human)
dTTP biosynthetic processThymidylate synthaseHomo sapiens (human)
circadian rhythmThymidylate synthaseHomo sapiens (human)
response to xenobiotic stimulusThymidylate synthaseHomo sapiens (human)
response to toxic substanceThymidylate synthaseHomo sapiens (human)
negative regulation of translationThymidylate synthaseHomo sapiens (human)
uracil metabolic processThymidylate synthaseHomo sapiens (human)
methylationThymidylate synthaseHomo sapiens (human)
response to progesteroneThymidylate synthaseHomo sapiens (human)
response to vitamin AThymidylate synthaseHomo sapiens (human)
response to cytokineThymidylate synthaseHomo sapiens (human)
tetrahydrofolate interconversionThymidylate synthaseHomo sapiens (human)
response to ethanolThymidylate synthaseHomo sapiens (human)
response to organophosphorusThymidylate synthaseHomo sapiens (human)
developmental growthThymidylate synthaseHomo sapiens (human)
cartilage developmentThymidylate synthaseHomo sapiens (human)
response to glucocorticoidThymidylate synthaseHomo sapiens (human)
response to folic acidThymidylate synthaseHomo sapiens (human)
intestinal epithelial cell maturationThymidylate synthaseHomo sapiens (human)
DNA biosynthetic processThymidylate synthaseHomo sapiens (human)
liver regenerationThymidylate synthaseHomo sapiens (human)
dTMP biosynthetic processThymidylate synthaseEscherichia coli K-12
response to radiationThymidylate synthaseEscherichia coli K-12
dTMP biosynthetic processThymidylate synthaseEscherichia coli K-12
dTTP biosynthetic processThymidylate synthaseEscherichia coli K-12
regulation of translationThymidylate synthaseEscherichia coli K-12
nucleotide biosynthetic processThymidylate synthaseEscherichia coli K-12
methylationThymidylate synthaseEscherichia coli K-12
[Information is prepared from geneontology information from the June-17-2024 release]

Molecular Functions (8)

Processvia Protein(s)Taxonomy
mRNA regulatory element binding translation repressor activityThymidylate synthaseHomo sapiens (human)
thymidylate synthase activityThymidylate synthaseHomo sapiens (human)
folic acid bindingThymidylate synthaseHomo sapiens (human)
protein homodimerization activityThymidylate synthaseHomo sapiens (human)
sequence-specific mRNA bindingThymidylate synthaseHomo sapiens (human)
magnesium ion bindingThymidylate synthaseEscherichia coli K-12
RNA bindingThymidylate synthaseEscherichia coli K-12
thymidylate synthase activityThymidylate synthaseEscherichia coli K-12
methyltransferase activityThymidylate synthaseEscherichia coli K-12
protein homodimerization activityThymidylate synthaseEscherichia coli K-12
[Information is prepared from geneontology information from the June-17-2024 release]

Ceullar Components (6)

Processvia Protein(s)Taxonomy
nucleusThymidylate synthaseHomo sapiens (human)
cytoplasmThymidylate synthaseHomo sapiens (human)
mitochondrionThymidylate synthaseHomo sapiens (human)
mitochondrial inner membraneThymidylate synthaseHomo sapiens (human)
mitochondrial matrixThymidylate synthaseHomo sapiens (human)
cytosolThymidylate synthaseHomo sapiens (human)
mitochondrionThymidylate synthaseHomo sapiens (human)
cytosolThymidylate synthaseHomo sapiens (human)
cytoplasmThymidylate synthaseEscherichia coli K-12
cytosolThymidylate synthaseEscherichia coli K-12
cytosolThymidylate synthaseEscherichia coli K-12
[Information is prepared from geneontology information from the June-17-2024 release]

Bioassays (5)

Assay IDTitleYearJournalArticle
AID1794808Fluorescence-based screening to identify small molecule inhibitors of Plasmodium falciparum apicoplast DNA polymerase (Pf-apPOL).2014Journal of biomolecular screening, Jul, Volume: 19, Issue:6
A High-Throughput Assay to Identify Inhibitors of the Apicoplast DNA Polymerase from Plasmodium falciparum.
AID1794808Fluorescence-based screening to identify small molecule inhibitors of Plasmodium falciparum apicoplast DNA polymerase (Pf-apPOL).
AID1799451Enzyme Assay from Article 10.1016/S1074-5521(99)80077-5: \\Structure-based discovery and in-parallel optimization of novel competitive inhibitors of thymidylate synthase.\\1999Chemistry & biology, May, Volume: 6, Issue:5
Structure-based discovery and in-parallel optimization of novel competitive inhibitors of thymidylate synthase.
AID1799474Inhibition Assay from Article 10.1016/S1074-5521(01)00067-9: \\Predicting and harnessing protein flexibility in the design of species-specific inhibitors of thymidylate synthase.\\2001Chemistry & biology, Oct, Volume: 8, Issue:10
Predicting and harnessing protein flexibility in the design of species-specific inhibitors of thymidylate synthase.
AID1159607Screen for inhibitors of RMI FANCM (MM2) intereaction2016Journal of biomolecular screening, Jul, Volume: 21, Issue:6
A High-Throughput Screening Strategy to Identify Protein-Protein Interaction Inhibitors That Block the Fanconi Anemia DNA Repair Pathway.
[information is prepared from bioassay data collected from National Library of Medicine (NLM), extracted Dec-2023]

Research

Studies (65)

TimeframeStudies, This Drug (%)All Drugs %
pre-199021 (32.31)18.7374
1990's16 (24.62)18.2507
2000's12 (18.46)29.6817
2010's12 (18.46)24.3611
2020's4 (6.15)2.80
[information is prepared from research data collected from National Library of Medicine (NLM), extracted Dec-2023]

Market Indicators

Research Demand Index: 33.92

According to the monthly volume, diversity, and competition of internet searches for this compound, as well the volume and growth of publications, there is estimated to be moderate demand-to-supply ratio for research on this compound.

MetricThis Compound (vs All)
Research Demand Index33.92 (24.57)
Research Supply Index4.19 (2.92)
Research Growth Index4.53 (4.65)
Search Engine Demand Index28.17 (26.88)
Search Engine Supply Index2.00 (0.95)

This Compound (33.92)

All Compounds (24.57)

Study Types

Publication TypeThis drug (%)All Drugs (%)
Trials0 (0.00%)5.53%
Reviews1 (1.54%)6.00%
Case Studies0 (0.00%)4.05%
Observational0 (0.00%)0.25%
Other64 (98.46%)84.16%
[information is prepared from research data collected from National Library of Medicine (NLM), extracted Dec-2023]