Assay ID | Title | Year | Journal | Article |
AID502946 | Inhibition of GEF-independent nucleotide exchange activity of prenylated Cdc42 assessed as protein dissociation from RhoGD11 complex at 100 uM after 3 mins in presence of 4 mM Mg2+ chelator EDTA | 2006 | Nature chemical biology, Jan, Volume: 2, Issue:1
| Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. |
AID502947 | Binding affinity to phosphatidylinositol 4,5-bisphosphate liposome at 50 uM by electron microscopy | 2006 | Nature chemical biology, Jan, Volume: 2, Issue:1
| Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. |
AID502930 | Inhibition of GEF-independent nucleotide exchange activity of prenylated Cdc42 after 3 mins in presence of 4 mM Mg2+ chelator EDTA | 2006 | Nature chemical biology, Jan, Volume: 2, Issue:1
| Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. |
AID502922 | Inhibition of CDC42-mediated actin polymerization in PIP2-stimulated Xenopus laevis egg extract after 6 mins by fluorescence assay | 2006 | Nature chemical biology, Jan, Volume: 2, Issue:1
| Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. |
AID502940 | Inhibition of CDC42-mediated actin polymerization in PIP2-stimulated Xenopus laevis egg extract at 0.1 to 100 uM by fluorescence assay | 2006 | Nature chemical biology, Jan, Volume: 2, Issue:1
| Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. |
AID502927 | Inhibition of Cdc42 binding to PIP2 liposomes at 100 uM after 10 mins | 2006 | Nature chemical biology, Jan, Volume: 2, Issue:1
| Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. |
AID502931 | Inhibition of GEF-independent nucleotide exchange activity of nonprenylated Cdc42 at 100 uM after 10 mins in presence of 4 mM EDTA | 2006 | Nature chemical biology, Jan, Volume: 2, Issue:1
| Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. |
AID502932 | Effect on Cdc42-mediated cellular response in human U373MG cells assessed as reorientation of golgi complex 15 uM after 6 hrs by epifluorescence assay | 2006 | Nature chemical biology, Jan, Volume: 2, Issue:1
| Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. |
AID502938 | Effect on transferrin translocation from golgi complex to endoplasmic reticulum of african green monkey BSC1 cells at 12.5 uM after 2.5 hrs by epifluorescence microscopy | 2006 | Nature chemical biology, Jan, Volume: 2, Issue:1
| Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. |
AID502929 | Increase in p75-GFP transport from golgi complex of MDCK cells at 10 uM after 20 mins by fluorescence assay | 2006 | Nature chemical biology, Jan, Volume: 2, Issue:1
| Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. |
AID502945 | Effect on nucleotide binding activity of PIP2-stimulated Cdc42 at 25 uM by liquid scintillation counting | 2006 | Nature chemical biology, Jan, Volume: 2, Issue:1
| Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. |
AID502933 | Inhibition of Cdc42 binding to rat liver golgi membrane at 10 to 150 uM by Western blot analysis | 2006 | Nature chemical biology, Jan, Volume: 2, Issue:1
| Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. |
AID502934 | Inhibition of VSVG-EGFP anterograde transport from endoplasmic reticulum to golgi complex of african green monkey BSC1 cells at 12.5 uM after 3 hrs by epifluorescence microscopy | 2006 | Nature chemical biology, Jan, Volume: 2, Issue:1
| Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. |
AID502925 | Effect on GST-PBD binding to recombinant nonprenylated Cdc42 in PIP2 liposome-stimulated Xenopus laevis egg extract at 50 uM by Western blot analysis | 2006 | Nature chemical biology, Jan, Volume: 2, Issue:1
| Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. |
AID502941 | Inhibition of actin polymerization in N-WASP VCA-stimulated Xenopus laevis egg extract at 20 uM after 1.5 mins by fluorescence assay | 2006 | Nature chemical biology, Jan, Volume: 2, Issue:1
| Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. |
AID502921 | Inhibition of VSVG-EGFP anterograde transport from endoplasmic reticulum to plasma membrane of african green monkey BSC1 cells after 3 hrs by epifluorescence microscopy | 2006 | Nature chemical biology, Jan, Volume: 2, Issue:1
| Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. |
AID502944 | Effect on GEF interaction with PIP2-stimulated Cdc42 at 25 uM by liquid scintillation counting | 2006 | Nature chemical biology, Jan, Volume: 2, Issue:1
| Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. |
AID502936 | Inhibition of GST-PBD binding to Cdc42 in Xenopus laevis egg extract assessed as Cdc42(GTPgammasS) activation at 50 uM treated before addition of GST-PBD by Western blot analysis | 2006 | Nature chemical biology, Jan, Volume: 2, Issue:1
| Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. |
AID502924 | Inhibition of PIP2 liposome-stimulated Cdc42 activation in Xenopus laevis egg extract assessed as inhibition of association of Cdc42(GTPgammaS) with GST-PBD at 1 to 25 uM by Western blot analysis | 2006 | Nature chemical biology, Jan, Volume: 2, Issue:1
| Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. |
AID502948 | Binding affinity to phosphatidylinositol 4,5-bisphosphate liposome assessed as dynamin2-stimulated GTP hydrolysis at 25 uM by scintillation counting | 2006 | Nature chemical biology, Jan, Volume: 2, Issue:1
| Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. |
AID502937 | Effect on perinuclear localization of trans-Golgi network in african green monkey BSC1 cells at 12.5 uM after 2.5 hrs by epifluorescence microscopy | 2006 | Nature chemical biology, Jan, Volume: 2, Issue:1
| Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. |
AID502935 | Inhibition of GST-PBD binding to Cdc42 in Xenopus laevis egg extract assessed as Cdc42(GTPgammasS) activation at 50 uM treated after addition of GST-PBD by Western blot analysis | 2006 | Nature chemical biology, Jan, Volume: 2, Issue:1
| Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. |
AID502928 | Decrease in NCAM-GFP transport from golgi complex of MDCK cells at 10 uM after 20 mins by fluorescence assay | 2006 | Nature chemical biology, Jan, Volume: 2, Issue:1
| Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. |
AID502939 | Effect on transferrin endocytosis in african green monkey BSC1 cells at 12.5 uM after 2.5 hrs by epifluorescence microscopy | 2006 | Nature chemical biology, Jan, Volume: 2, Issue:1
| Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. |
AID502943 | Inhibition of nucleotide exchange activity of PIP2-stimulated Cdc42 at 25 uM by liquid scintillation counting | 2006 | Nature chemical biology, Jan, Volume: 2, Issue:1
| Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro. |
[information is prepared from bioassay data collected from National Library of Medicine (NLM), extracted Dec-2023] |