Compounds > 2,3-dihydro-3beta-methoxy withaferin A
Page last updated: 2024-11-12

2,3-dihydro-3beta-methoxy withaferin A

Description Research Excerpts Clinical Trials Roles Classes Pathways Study Profile Bioassays Related Drugs Related Conditions Protein Interactions Research Growth Market Indicators

Description

2,3-dihydro-3beta-methoxy withaferin A : A withanolide that is 2,3-dihydrowithaferin A substituted by a beta-methoxy group at position 3. It has been isolated from the aerial parts of Physalis longifolia. [Chemical Entities of Biological Interest (ChEBI), Hastings J, Owen G, Dekker A, Ennis M, Kale N, Muthukrishnan V, Turner S, Swainston N, Mendes P, Steinbeck C. (2016). ChEBI in 2016: Improved services and an expanding collection of metabolites. Nucleic Acids Res]

FloraRankFlora DefinitionFamilyFamily Definition
PhysalisgenusA plant genus of the family SOLANACEAE. Members contain physalin and withangulatin.[MeSH]SolanaceaeA plant family of the order SOLANALES, class MAGNOLIOPSIDA. Among the most noted are POTATOES; TOMATOES; CAPSICUM (green and red peppers); TOBACCO; and BELLADONNA.[MeSH]

Cross-References

ID SourceID
PubMed CID10767792
CHEMBL ID1934583
CHEBI ID69121
SCHEMBL ID20547177

Synonyms (17)

Synonym
quresimine a
(3beta,4beta,5beta,6beta,22r)-4,27-dihydroxy-3-methoxy-5,6:22,26-diepoxyergost-24-ene-1,26-dione
2,3-dihydro-3beta-methoxy withaferin a
chebi:69121 ,
CHEMBL1934583 ,
3beta-methoxy-2,3-dihydrowithaferin a
Q27137461
SCHEMBL20547177
E88680
CS-0032497
HY-N5131
3|a-methoxy-2,3-dihydrowithaferin a
(1s,2r,5s,6s,7r,9r,11s,12s,15r,16s)-6-hydroxy-15-[(1s)-1-[(2r)-5-(hydroxymethyl)-4-methyl-6-oxo-2,3-dihydropyran-2-yl]ethyl]-5-methoxy-2,16-dimethyl-8-oxapentacyclo[9.7.0.02,7.07,9.012,16]octadecan-3-one
bdbm50572805
AKOS040760221
3??-methoxy-2,3-dihydrowithaferin a
FS-7248
[information is derived through text-mining from research data collected from National Library of Medicine (NLM), extracted Dec-2023]

Roles (2)

RoleDescription
metaboliteAny intermediate or product resulting from metabolism. The term 'metabolite' subsumes the classes commonly known as primary and secondary metabolites.
plant metaboliteAny eukaryotic metabolite produced during a metabolic reaction in plants, the kingdom that include flowering plants, conifers and other gymnosperms.
[role information is derived from Chemical Entities of Biological Interest (ChEBI), Hastings J, Owen G, Dekker A, Ennis M, Kale N, Muthukrishnan V, Turner S, Swainston N, Mendes P, Steinbeck C. (2016). ChEBI in 2016: Improved services and an expanding collection of metabolites. Nucleic Acids Res]

Drug Classes (8)

ClassDescription
delta-lactoneA lactone having a six-membered lactone ring.
27-hydroxy steroid
4-hydroxy steroidAny steroid bearing a 4-hydroxy substituent.
ergostanoid
primary alcoholA primary alcohol is a compound in which a hydroxy group, -OH, is attached to a saturated carbon atom which has either three hydrogen atoms attached to it or only one other carbon atom and two hydrogen atoms attached to it.
secondary alcoholA secondary alcohol is a compound in which a hydroxy group, -OH, is attached to a saturated carbon atom which has two other carbon atoms attached to it.
withanolideAny steroid lactone that is a C28 steroid with a modified side chain forming a lactone ring and its substituted derivatives.
epoxy steroidAny steroid whose structure includes an epoxy group.
[compound class information is derived from Chemical Entities of Biological Interest (ChEBI), Hastings J, Owen G, Dekker A, Ennis M, Kale N, Muthukrishnan V, Turner S, Swainston N, Mendes P, Steinbeck C. (2016). ChEBI in 2016: Improved services and an expanding collection of metabolites. Nucleic Acids Res]

Protein Targets (1)

Inhibition Measurements

ProteinTaxonomyMeasurementAverageMin (ref.)Avg (ref.)Max (ref.)Bioassay(s)
[prepared from compound, protein, and bioassay information from National Library of Medicine (NLM), extracted Dec-2023]

Ceullar Components (1)

Processvia Protein(s)Taxonomy
nucleoplasmNuclear receptor ROR-alphaMus musculus (house mouse)
[Information is prepared from geneontology information from the June-17-2024 release]

Bioassays (89)

Assay IDTitleYearJournalArticle
AID1492770Effect on p21 WAF1 expression level in human TIG3 cells at 0.3 to 3 uM by immunoblot analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492801Induction of AKT/p38/p44 MAPK-mediated survivin expression level in H2O2 treated human TIG3 cells at 0.6 uM pretreated with H2O2 for 2 hrs followed by compound addition by immunoblot analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID639260Cytotoxicity against human MDA1986 cells after 72 hrs by MTS assay2011Journal of natural products, Dec-27, Volume: 74, Issue:12
Cytotoxic withanolide constituents of Physalis longifolia.
AID1492781Cytoprotective activity against doxorubicin-induced stress in human TIG3 cells assessed as increase in cell survival pretreated with doxorubicin for 24 hrs followed by compound addition measured after 2 days by crystal violet staining based assay2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492799Induction of p38 MAPK phosphorylation at Thr180/Tyr182 residues in H2O2 treated human TIG3 cells at 0.6 uM pretreated with H2O2 for 2 hrs followed by compound addition by immunoblot analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1768106Elongation of circadian rhythms in dexamethasone-induced mouse Sarcoma-180 cells assessed as increase in Bmal1 period time at 5 uM measured for 1 mins at 10 min interval up to 96 hrs by bioluminescence based Bmal1 promoter-reporter gene assay (Rvb = 25.702021Journal of natural products, 07-23, Volume: 84, Issue:7
Withanolide Derivative 2,3-Dihydro-3β-methoxy Withaferin-A Modulates the Circadian Clock via Interaction with RAR-Related Orphan Receptor α (RORa).
AID1492791Induction of ERK1 phosphorylation at Thr202 residues in H2O2 treated human TIG3 cells pretreated with H2O2 for 2 hrs followed by compound addition by immuno fluorescence staining based analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492772Decrease in keap1 expression level in human TIG3 cells at 0.3 to 3 uM by immunoblot analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1768105Cytotoxicity against mouse Sarcoma-180 cells assessed as reduction in cell proliferation at 1 uM measured up to 5 days by WST-8 assay2021Journal of natural products, 07-23, Volume: 84, Issue:7
Withanolide Derivative 2,3-Dihydro-3β-methoxy Withaferin-A Modulates the Circadian Clock via Interaction with RAR-Related Orphan Receptor α (RORa).
AID1768102Induction of Bmal1 promotor activity in dexamethasone-induced mouse NIH3T3 cells transfected with Bmal1 promotor-reporter plasmids at 5 uM measured after 24 hrs by bioluminescence assay relative to control2021Journal of natural products, 07-23, Volume: 84, Issue:7
Withanolide Derivative 2,3-Dihydro-3β-methoxy Withaferin-A Modulates the Circadian Clock via Interaction with RAR-Related Orphan Receptor α (RORa).
AID1492804Induction of ERK2 phosphorylation at Tyr204 residues in H2O2 treated human TIG3 cells at 0.6 uM pretreated with H2O2 for 2 hrs followed by compound addition by immunoblot analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492814Cytoprotective activity against H2O2-induced oxidative stress in human TIG3 cells assessed as decrease in protein disulfide isomerase expression level at 0.6 uM pretreated with H2O2 for 2 hrs followed by compound addition by immuno fluorescence staining b2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1768103Transcriptional upregulation of Bmal1 mRNA expression in dexamethasone-induced mouse Sarcoma-180 cells at 1 uM measured after 24 hrs by RT-qPCR analysis relative to control2021Journal of natural products, 07-23, Volume: 84, Issue:7
Withanolide Derivative 2,3-Dihydro-3β-methoxy Withaferin-A Modulates the Circadian Clock via Interaction with RAR-Related Orphan Receptor α (RORa).
AID1768113Elongation of circadian rhythms in dexamethasone-induced mouse embryonic fibroblasts expressing PER2-luc gene assessed as increase in PER2 period time at 25 uM measured for 1 mins at 10 min interval up to 96 hrs by bioluminescence based PER2 promoter-repo2021Journal of natural products, 07-23, Volume: 84, Issue:7
Withanolide Derivative 2,3-Dihydro-3β-methoxy Withaferin-A Modulates the Circadian Clock via Interaction with RAR-Related Orphan Receptor α (RORa).
AID1492773Decrease in Nrf2 expression level in human TIG3 cells at 0.3 to 3 uM by immunoblot analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492768Induction of oxidative stress in human TIG3 cells assessed as increase in GADD45alpha mRNA levels by RT-PCR analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID639259Cytotoxicity against human JMAR cells after 72 hrs by MTS assay2011Journal of natural products, Dec-27, Volume: 74, Issue:12
Cytotoxic withanolide constituents of Physalis longifolia.
AID1768112Elongation of circadian rhythms in dexamethasone-induced mouse embryonic fibroblasts expressing PER2-luc gene assessed as increase in PER2 period time at 5 uM measured for 1 mins at 10 min interval up to 96 hrs by bioluminescence based PER2 promoter-repor2021Journal of natural products, 07-23, Volume: 84, Issue:7
Withanolide Derivative 2,3-Dihydro-3β-methoxy Withaferin-A Modulates the Circadian Clock via Interaction with RAR-Related Orphan Receptor α (RORa).
AID1492780Cytoprotective activity against UV irradiation-induced stress in human TIG3 cells assessed as increase in cell survival irradiated with 50 mJ/cm2 of UV radiations followed by compound addition measured after 2 days by crystal violet staining based assay2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1768101Induction of Bmal1 promotor activity in dexamethasone-induced mouse NIH3T3 cells transfected with Bmal1 promotor-reporter plasmids at 15 uM measured after 24 hrs by bioluminescence assay relative to control2021Journal of natural products, 07-23, Volume: 84, Issue:7
Withanolide Derivative 2,3-Dihydro-3β-methoxy Withaferin-A Modulates the Circadian Clock via Interaction with RAR-Related Orphan Receptor α (RORa).
AID1768096Transcriptional upregulation of Nrld1 mRNA expression in dexamethasone-induced mouse NIH3T3 cells at 15 uM measured after 24 hrs by qPCR analysis relative to control2021Journal of natural products, 07-23, Volume: 84, Issue:7
Withanolide Derivative 2,3-Dihydro-3β-methoxy Withaferin-A Modulates the Circadian Clock via Interaction with RAR-Related Orphan Receptor α (RORa).
AID1492784Cytoprotective activity against UV irradiation-induced stress in human TIG3 cells assessed as increase in cell survival at 3 uM irradiated with 50 mJ/cm2 of UV radiations followed by compound addition measured after 72 hrs by crystal violet staining based2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492794Induction of ERK1 phosphorylation at Thr202 residues in UV-irradiated human TIG3 cells using 50 mJ/cm2 of UV radiations followed by compound addition by immuno fluorescence staining based analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492792Induction of ERK2 phosphorylation at Tyr204 residues in H2O2 treated human TIG3 cells pretreated with H2O2 for 2 hrs followed by compound addition by immuno fluorescence staining based analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492763Cytotoxicity against human TIG3 cells assessed as induction of morphological changes at 0.3 to 1.5 uM after 48 hrs by phase contrast microscopy2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492764Cytotoxicity against human TIG3 cells assessed as decrease in ROS generation at 1.5 to 3 uM after 48 hrs2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492790Induction of p38 MAPK phosphorylation at Thr180/Tyr182 residues in H2O2 treated human TIG3 cells pretreated with H2O2 for 2 hrs followed by compound addition by immuno fluorescence staining based analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492787Cytoprotective activity against H2O2-induced oxidative stress in human TIG3 cells assessed as decrease in CARF level pretreated with H2O2 for 2 hrs followed by compound addition by immuno fluorescence staining based assay2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492776Activation of keap1 in human TIG3 cells assessed as Nrf2 nuclear accumulation at 0.6 uM by immuno staining analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID639261Cytotoxicity against human MCR5 cells after 72 hrs by MTS assay2011Journal of natural products, Dec-27, Volume: 74, Issue:12
Cytotoxic withanolide constituents of Physalis longifolia.
AID1492793Induction of p38 MAPK phosphorylation at Thr180/Tyr182 residues in UV-irradiated human TIG3 cells using 50 mJ/cm2 of UV radiations followed by compound addition by immuno fluorescence staining based analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1379656Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability using compound addition to cell culture cells in lag phase of growth and incubated for 48 hrs by MTT assay2017European journal of medicinal chemistry, Nov-10, Volume: 140Structure-based design, synthesis, and biological evaluation of withaferin A-analogues as potent apoptotic inducers.
AID1768107Elongation of circadian rhythms in dexamethasone-induced mouse NIH3T3 cells assessed as increase in Bmal1 period time at 1 uM measured for 1 mins at 10 min interval up to 96 hrs by dual luciferase reporter gene assay (Rvb = 25.02 +/- 0.15 hrs)2021Journal of natural products, 07-23, Volume: 84, Issue:7
Withanolide Derivative 2,3-Dihydro-3β-methoxy Withaferin-A Modulates the Circadian Clock via Interaction with RAR-Related Orphan Receptor α (RORa).
AID1492810Cytoprotective activity against H2O2-induced oxidative stress in human TIG3 cells assessed as increase in full length PARP level at 0.6 uM pretreated with H2O2 for 2 hrs followed by compound addition by immunoblot analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492761Cytotoxicity against human TIG3 cells assessed as inhibition of colony formation by crystal violet staining based assay2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492811Cytoprotective activity against H2O2-induced oxidative stress in human TIG3 cells assessed as decrease in gammaH2AX level at 0.6 uM pretreated with H2O2 for 2 hrs followed by compound addition by immuno fluorescence staining based analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492779Cytoprotective activity against H2O2-induced oxidative stress in human TIG3 cells assessed as increase in cell survival at 3 uM pretreated with H2O2 for 2 hrs followed by compound addition measured after 2 days by crystal violet staining based assay2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1768116Cytotoxicity against mouse Sarcoma-180 cells assessed as reduction in cell proliferation at 5 uM measured up to 5 days by WST-8 assay2021Journal of natural products, 07-23, Volume: 84, Issue:7
Withanolide Derivative 2,3-Dihydro-3β-methoxy Withaferin-A Modulates the Circadian Clock via Interaction with RAR-Related Orphan Receptor α (RORa).
AID1492767Induction of oxidative stress against human TIG3 cells assessed as increase in CARF mRNA levels by RT-PCR analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492789Cytoprotective activity against UV irradiation-induced stress in human TIG3 cells assessed as decrease in CARF level irradiated with 50 mJ/cm2 of UV radiations followed by compound addition by immuno fluorescence staining based assay2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492809Cytoprotective activity against H2O2-induced oxidative stress in human TIG3 cells assessed as increase in Bcl2 level at 0.6 uM pretreated with H2O2 for 2 hrs followed by compound addition by immunoblot analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1240123Cytotoxicity against human PANC1 cells after 24 hrs by FMCA2015Bioorganic & medicinal chemistry letters, Sep-01, Volume: 25, Issue:17
Hedgehog inhibitors from Withania somnifera.
AID1768100Transcriptional upregulation of Bmal1 mRNA expression in dexamethasone-induced mouse Sarcoma-180 cells at 5 uM measured after 24 hrs by RT-qPCR analysis relative to control2021Journal of natural products, 07-23, Volume: 84, Issue:7
Withanolide Derivative 2,3-Dihydro-3β-methoxy Withaferin-A Modulates the Circadian Clock via Interaction with RAR-Related Orphan Receptor α (RORa).
AID1768117Elongation of circadian rhythms in dexamethasone-induced mouse Sarcoma-180 cells assessed as increase in Bmal1 period length measured for 1 mins at 10 min interval up to 96 hrs by bioluminescence based Bmal1 promoter-reporter gene assay2021Journal of natural products, 07-23, Volume: 84, Issue:7
Withanolide Derivative 2,3-Dihydro-3β-methoxy Withaferin-A Modulates the Circadian Clock via Interaction with RAR-Related Orphan Receptor α (RORa).
AID1492813Cytoprotective activity against H2O2-induced oxidative stress in human TIG3 cells assessed as decrease in 53BP1 level at 0.6 uM pretreated with H2O2 for 2 hrs followed by compound addition by immuno fluorescence staining based analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1768097Transcriptional upregulation of Nrld1 mRNA expression in dexamethasone-induced mouse NIH3T3 cells at 5 uM measured after 24 hrs by qPCR analysis relative to control2021Journal of natural products, 07-23, Volume: 84, Issue:7
Withanolide Derivative 2,3-Dihydro-3β-methoxy Withaferin-A Modulates the Circadian Clock via Interaction with RAR-Related Orphan Receptor α (RORa).
AID1768108Elongation of circadian rhythms in dexamethasone-induced mouse NIH3T3 cells assessed as increase in Bmal1 period time at 5 uM measured for 1 mins at 10 min interval up to 96 hrs by dual luciferase reporter gene assay (Rvb = 25.02 +/- 0.15 hrs)2021Journal of natural products, 07-23, Volume: 84, Issue:7
Withanolide Derivative 2,3-Dihydro-3β-methoxy Withaferin-A Modulates the Circadian Clock via Interaction with RAR-Related Orphan Receptor α (RORa).
AID1379652Cytotoxicity against human A549 cells assessed as reduction in cell viability using compound addition to cell culture cells in lag phase of growth and incubated for 48 hrs by MTT assay2017European journal of medicinal chemistry, Nov-10, Volume: 140Structure-based design, synthesis, and biological evaluation of withaferin A-analogues as potent apoptotic inducers.
AID1492760Cytotoxicity against human TIG3 cells assessed as cell death at 0.6 uM2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492783Cytoprotective activity against etoposide-induced stress in human TIG3 cells assessed as increase in cell survival pretreated with etoposide for 24 hrs followed by compound addition measured after 2 days by crystal violet staining based assay2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492786Cytoprotective activity against H2O2-induced oxidative stress in human TIG3 cells assessed as decrease in p53 level pretreated with H2O2 for 2 hrs followed by compound addition by immuno fluorescence staining based assay2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1768111Elongation of circadian rhythms in dexamethasone-induced mouse embryonic fibroblasts expressing PER2-luc gene assessed as increase in PER2 period time at 1.67 uM measured for 1 mins at 10 min interval up to 96 hrs by bioluminescence based PER2 promoter-re2021Journal of natural products, 07-23, Volume: 84, Issue:7
Withanolide Derivative 2,3-Dihydro-3β-methoxy Withaferin-A Modulates the Circadian Clock via Interaction with RAR-Related Orphan Receptor α (RORa).
AID1492769Effect on p53 expression level in human TIG3 cells at 0.3 to 3 uM by immunoblot analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492788Cytoprotective activity against UV irradiation-induced stress in human TIG3 cells assessed as decrease in p53 level irradiated with 50 mJ/cm2 of UV radiations followed by compound addition by immuno fluorescence staining based assay2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1768095Inverse agonist activity at RORalpha in mouse NIH3T3 cells incubated for 24 hrs by luminescence based assay2021Journal of natural products, 07-23, Volume: 84, Issue:7
Withanolide Derivative 2,3-Dihydro-3β-methoxy Withaferin-A Modulates the Circadian Clock via Interaction with RAR-Related Orphan Receptor α (RORa).
AID1240125Cytotoxicity against human MCF7 cells after 24 hrs by FMCA2015Bioorganic & medicinal chemistry letters, Sep-01, Volume: 25, Issue:17
Hedgehog inhibitors from Withania somnifera.
AID1492782Cytoprotective activity against cisplatin-induced stress in human TIG3 cells assessed as increase in cell survival pretreated with cisplatin for 24 hrs followed by compound addition measured after 2 days by crystal violet staining based assay2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492757Cytotoxicity against human WI38 cells assessed as reduction in cell viability at 1 to 10 uM after 72 hrs2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492802Induction of ERK1 phosphorylation at Thr202 residues in H2O2 treated human TIG3 cells at 0.6 uM pretreated with H2O2 for 2 hrs followed by compound addition by immunoblot analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1768110Elongation of circadian rhythms in dexamethasone-induced mouse embryonic fibroblasts expressing PER2-luc gene assessed as increase in PER2 period time at 1 uM measured for 1 mins at 10 min interval up to 96 hrs by bioluminescence based PER2 promoter-repor2021Journal of natural products, 07-23, Volume: 84, Issue:7
Withanolide Derivative 2,3-Dihydro-3β-methoxy Withaferin-A Modulates the Circadian Clock via Interaction with RAR-Related Orphan Receptor α (RORa).
AID1768115Transcriptional upregulation of Bmal1 mRNA expression in dexamethasone-induced mouse NIH3T3 cells at 5 uM measured after 24 hrs by RT-qPCR analysis relative to control2021Journal of natural products, 07-23, Volume: 84, Issue:7
Withanolide Derivative 2,3-Dihydro-3β-methoxy Withaferin-A Modulates the Circadian Clock via Interaction with RAR-Related Orphan Receptor α (RORa).
AID1768104Cytotoxicity against mouse Sarcoma-180 cells assessed as reduction in cell proliferation by measuring growth rate at 1 uM measured after 3 days by WST-8 assay relative to control2021Journal of natural products, 07-23, Volume: 84, Issue:7
Withanolide Derivative 2,3-Dihydro-3β-methoxy Withaferin-A Modulates the Circadian Clock via Interaction with RAR-Related Orphan Receptor α (RORa).
AID1379654Cytotoxicity against human MCF7 cells assessed as reduction in cell viability using compound addition to cell culture cells in lag phase of growth and incubated for 48 hrs by MTT assay2017European journal of medicinal chemistry, Nov-10, Volume: 140Structure-based design, synthesis, and biological evaluation of withaferin A-analogues as potent apoptotic inducers.
AID1492812Cytoprotective activity against H2O2-induced oxidative stress in human TIG3 cells assessed as decrease in phosphorylated ATM level at 0.6 uM pretreated with H2O2 for 2 hrs followed by compound addition by immuno fluorescence staining based analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1240124Cytotoxicity against human DU145 cells after 24 hrs by FMCA2015Bioorganic & medicinal chemistry letters, Sep-01, Volume: 25, Issue:17
Hedgehog inhibitors from Withania somnifera.
AID1379655Cytotoxicity against human MCF7 cells assessed as reduction in cell viability using compound addition to cell culture cells in log phase of growth and incubated for 48 hrs by MTT assay2017European journal of medicinal chemistry, Nov-10, Volume: 140Structure-based design, synthesis, and biological evaluation of withaferin A-analogues as potent apoptotic inducers.
AID1240126Cytotoxicity against mouse C3H10T1/2 cells after 24 hrs by FMCA2015Bioorganic & medicinal chemistry letters, Sep-01, Volume: 25, Issue:17
Hedgehog inhibitors from Withania somnifera.
AID1492775Induction of oxidative stress in human TIG3 cells assessed as increase in p53 expression level at 0.6 uM by immunoblot analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492785Cytoprotective activity against H2O2-induced oxidative stress in human TIG3 cells assessed as increase in cell survival at 3 uM pretreated with H2O2 for 2 hrs followed by compound addition measured after 72 hrs by crystal violet staining based assay2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1768099Transcriptional upregulation of Rora mRNA expression in dexamethasone-induced mouse NIH3T3 cells at 5 uM measured after 24 hrs by qPCR analysis relative to control2021Journal of natural products, 07-23, Volume: 84, Issue:7
Withanolide Derivative 2,3-Dihydro-3β-methoxy Withaferin-A Modulates the Circadian Clock via Interaction with RAR-Related Orphan Receptor α (RORa).
AID1492771Effect on mortalin expression level in human TIG3 cells at 0.3 to 3 uM by immuno fluorescence analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1379651Cytotoxicity against human HeLa cells assessed as reduction in cell viability using compound addition to cell culture cells in log phase of growth and incubated for 48 hrs by MTT assay2017European journal of medicinal chemistry, Nov-10, Volume: 140Structure-based design, synthesis, and biological evaluation of withaferin A-analogues as potent apoptotic inducers.
AID1379653Cytotoxicity against human A549 cells assessed as reduction in cell viability using compound addition to cell culture cells in log phase of growth and incubated for 48 hrs by MTT assay2017European journal of medicinal chemistry, Nov-10, Volume: 140Structure-based design, synthesis, and biological evaluation of withaferin A-analogues as potent apoptotic inducers.
AID1379657Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability using compound addition to cell culture cells in log phase of growth and incubated for 48 hrs by MTT assay2017European journal of medicinal chemistry, Nov-10, Volume: 140Structure-based design, synthesis, and biological evaluation of withaferin A-analogues as potent apoptotic inducers.
AID1768114Transcriptional upregulation of Bmal1 mRNA expression in dexamethasone-induced mouse NIH3T3 cells at 1 uM measured after 24 hrs by RT-qPCR analysis relative to control2021Journal of natural products, 07-23, Volume: 84, Issue:7
Withanolide Derivative 2,3-Dihydro-3β-methoxy Withaferin-A Modulates the Circadian Clock via Interaction with RAR-Related Orphan Receptor α (RORa).
AID1492806Induction of AKT-mediated beta-catenin expression level in H2O2 treated human TIG3 cells at 0.6 uM pretreated with H2O2 for 2 hrs followed by compound addition by immunoblot analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1240122Inhibition of hedgehog/GLI1-mediated transcriptional activity in human HaCaT cells after 12 hrs by luciferase assay2015Bioorganic & medicinal chemistry letters, Sep-01, Volume: 25, Issue:17
Hedgehog inhibitors from Withania somnifera.
AID639257Cytotoxicity against mouse B16F10 cells after 72 hrs by MTS assay2011Journal of natural products, Dec-27, Volume: 74, Issue:12
Cytotoxic withanolide constituents of Physalis longifolia.
AID1492756Cytotoxicity against human TIG3 cells assessed as reduction in cell viability at 1 to 10 uM after 72 hrs2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492795Induction of ERK2 phosphorylation at Tyr204 residues in UV-irradiated human TIG3 cells using 50 mJ/cm2 of UV radiations followed by compound addition by immuno fluorescence staining based analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1379650Cytotoxicity against human HeLa cells assessed as reduction in cell viability using compound addition to cell culture cells in lag phase of growth and incubated for 48 hrs by MTT assay2017European journal of medicinal chemistry, Nov-10, Volume: 140Structure-based design, synthesis, and biological evaluation of withaferin A-analogues as potent apoptotic inducers.
AID1768098Transcriptional upregulation of Rora mRNA expression in dexamethasone-induced mouse NIH3T3 cells at 15 uM measured after 24 hrs by qPCR analysis relative to control2021Journal of natural products, 07-23, Volume: 84, Issue:7
Withanolide Derivative 2,3-Dihydro-3β-methoxy Withaferin-A Modulates the Circadian Clock via Interaction with RAR-Related Orphan Receptor α (RORa).
AID639258Cytotoxicity against human SK-MEL-28 cells after 72 hrs by MTS assay2011Journal of natural products, Dec-27, Volume: 74, Issue:12
Cytotoxic withanolide constituents of Physalis longifolia.
AID1768109Elongation of circadian rhythms in dexamethasone-induced mouse Sarcoma-180 cells assessed as increase in Bmal1 period length at 1 to 5 uM measured for 1 mins at 10 min interval up to 96 hrs by bioluminescence based Bmal1 promoter-reporter gene assay2021Journal of natural products, 07-23, Volume: 84, Issue:7
Withanolide Derivative 2,3-Dihydro-3β-methoxy Withaferin-A Modulates the Circadian Clock via Interaction with RAR-Related Orphan Receptor α (RORa).
AID1492815Cytoprotective activity against human TIG3 cells assessed as increase in cell viability at 1.5 to 3 uM pretreated with Wi-A at 0.3 uM for 24 hrs followed by compound addition measured after 48 hrs2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492766Cytotoxicity against human TIG3 cells assessed as induction of senescence at 70 to 72 population doubling at 0.5 uM2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492762Cytotoxicity against human WI38 cells assessed as inhibition of colony formation by crystal violet staining based assay2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492797Induction of Akt phosphorylation at Ser473 residues in H2O2 treated human TIG3 cells at 0.6 uM pretreated with H2O2 for 2 hrs followed by compound addition by immunoblot analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
AID1492774Induction of oxidative stress in human TIG3 cells assessed as increase in CARF expression level at 0.6 uM by immunoblot analysis2017Journal of natural products, 10-27, Volume: 80, Issue:10
2,3-Dihydro-3β-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity.
[information is prepared from bioassay data collected from National Library of Medicine (NLM), extracted Dec-2023]

Research

Studies (5)

TimeframeStudies, This Drug (%)All Drugs %
pre-19900 (0.00)18.7374
1990's0 (0.00)18.2507
2000's0 (0.00)29.6817
2010's4 (80.00)24.3611
2020's1 (20.00)2.80
[information is prepared from research data collected from National Library of Medicine (NLM), extracted Dec-2023]

Market Indicators

Research Demand Index: 12.80

According to the monthly volume, diversity, and competition of internet searches for this compound, as well the volume and growth of publications, there is estimated to be weak demand-to-supply ratio for research on this compound.

MetricThis Compound (vs All)
Research Demand Index12.80 (24.57)
Research Supply Index1.79 (2.92)
Research Growth Index4.59 (4.65)
Search Engine Demand Index0.00 (26.88)
Search Engine Supply Index0.00 (0.95)

This Compound (12.80)

All Compounds (24.57)

Study Types

Publication TypeThis drug (%)All Drugs (%)
Trials0 (0.00%)5.53%
Reviews0 (0.00%)6.00%
Case Studies0 (0.00%)4.05%
Observational0 (0.00%)0.25%
Other5 (100.00%)84.16%
[information is prepared from research data collected from National Library of Medicine (NLM), extracted Dec-2023]
SubstanceRelationship StrengthStudiesTrialsClassesRoles
withaferin a
withaferin A: an antiestrogen and phytogenic antineoplastic agent isolated from leaves of Withania somnifera Dun.; structure. withaferin A : A withanolide that is 5,6:22,26-diepoxyergosta-2,24-diene-1,26-dione substituted by hydroxy groups at positions 4 and 27 (the 4beta,5beta,6beta,22R stereoisomer). Isolated from Physalis longifolia, it exhibits cytotoxic activity.		2.883027-hydroxy steroid;
4-hydroxy steroid;
delta-lactone;
enone;
epoxy steroid;
ergostanoid;
primary alcohol;
secondary alcohol;
withanolide		antineoplastic agent;
apoptosis inducer
7-dehydrocholesterol
[no description available]		2.31103beta-hydroxy-Delta(5)-steroid;
3beta-sterol;
cholestanoid;
Delta(5),Delta(7)-sterol		human metabolite;
mouse metabolite
dactinomycin
Dactinomycin: A compound composed of a two CYCLIC PEPTIDES attached to a phenoxazine that is derived from STREPTOMYCES parvullus. It binds to DNA and inhibits RNA synthesis (transcription), with chain elongation more sensitive than initiation, termination, or release. As a result of impaired mRNA production, protein synthesis also declines after dactinomycin therapy. (From AMA Drug Evaluations Annual, 1993, p2015)		2.1510actinomycinmutagen
mercaptopurine
Mercaptopurine: An antimetabolite antineoplastic agent with immunosuppressant properties. It interferes with nucleic acid synthesis by inhibiting purine metabolism and is used, usually in combination with other drugs, in the treatment of or in remission maintenance programs for leukemia.. purine-6-thiol : A thiol that is the tautomer of mercaptopurine.. mercaptopurine : A member of the class of purines that is 6,7-dihydro-1H-purine carrying a thione group at position 6. An adenine analogue, it is used in the treatment of acute lymphocytic leukemia (ALL), chronic myeloid leukemia (CML), Crohn's disease, and ulcerative colitis.		2.1510aryl thiol;
purines;
thiocarbonyl compound		anticoronaviral agent;
antimetabolite;
antineoplastic agent
2,3-dihydrowithaferin A
2,3-dihydrowithaferin A : A withanolide that is the 2,3-dihydro derivative of withaferin A. It has been isolated from the aerial parts of Physalis longifolia.		2.793027-hydroxy steroid;
4-hydroxy steroid;
delta-lactone;
epoxy steroid;
ergostanoid;
primary alcohol;
secondary alcohol;
withanolide		plant metabolite
2,3-dihydro-3beta-O-sulfate withaferin A
2,3-dihydro-3beta-O-sulfate withaferin A : A withanolide that is 2,3-dihydrowithaferin A substituted by a sulfoxy group at position 3. Isolated from Physalis longifolia, it exhibits antineoplastic activity.		2.492027-hydroxy steroid;
4-hydroxy steroid;
delta-lactone;
epoxy steroid;
ergostanoid;
primary alcohol;
steroid sulfate;
withanolide		antineoplastic agent;
metabolite;
plant metabolite
nimorazole
[no description available]		2.4920
ConditionIndicatedRelationship StrengthStudiesTrials
Carcinoma, Epidermoid
[description not available]		02.0610
Carcinoma, Squamous Cell
A carcinoma derived from stratified SQUAMOUS EPITHELIAL CELLS. It may also occur in sites where glandular or columnar epithelium is normally present. (From Stedman, 25th ed)		02.0610
Benign Neoplasms
[description not available]		02.1110
Neoplasms
New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.		02.1110