Page last updated: 2024-10-24

protein-lysine 6-oxidase activity

Definition

Target type: molecularfunction

Catalysis of the reaction: peptidyl-L-lysyl-peptide + H2O + O2 = peptidyl-allysyl-peptide + NH3 + hydrogen peroxide. [EC:1.4.3.13]

Protein-lysine 6-oxidase activity catalyzes the oxidation of the ε-amino group of lysine residues in proteins to the corresponding α-aminoadipic-δ-semialdehyde, with the concomitant reduction of molecular oxygen to hydrogen peroxide. This enzymatic activity is typically associated with lysine-specific demethylases (LSDs), which play crucial roles in epigenetic regulation by controlling the methylation status of lysine residues in histones and other proteins. The reaction mechanism involves the formation of a covalent intermediate between the enzyme and the lysine substrate, followed by the removal of the methyl group from the lysine residue and its oxidation to α-aminoadipic-δ-semialdehyde. The enzyme's active site contains a non-heme iron cofactor, which is essential for catalysis. This iron atom is coordinated by a conserved histidine residue and a carboxylate group, and it undergoes redox cycling during the reaction. The activity of protein-lysine 6-oxidase is tightly regulated by various factors, including substrate availability, cofactor concentration, and post-translational modifications. Disruption of protein-lysine 6-oxidase activity can lead to dysregulation of gene expression and cellular function, contributing to various diseases.'
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Proteins (5)

ProteinDefinitionTaxonomy
Protein-lysine 6-oxidase[no definition available]Bos taurus (cattle)
Lysyl oxidase homolog 2A lysyl oxidase homolog 2 that is encoded in the genome of human. [PRO:DNx, UniProtKB:Q9Y4K0]Homo sapiens (human)
Lysyl oxidase homolog 4A lysyl oxidase homolog 4 that is encoded in the genome of human. [PRO:DNx, UniProtKB:Q96JB6]Homo sapiens (human)
Lysyl oxidase homolog 3A lysyl oxidase homolog 3 that is encoded in the genome of human. [PRO:DNx, UniProtKB:P58215]Homo sapiens (human)
Protein-lysine 6-oxidaseA protein-lysine 6-oxidase that is encoded in the genome of human. [PRO:DNx, UniProtKB:P28300]Homo sapiens (human)

Compounds (15)

CompoundDefinitionClassesRoles
phenethylamine2-phenylethylamine : A phenylethylamine having the phenyl substituent at the 2-position.

phenethylamine: RN given refers to parent cpd; structure in Merck Index, 9th ed, #7016
alkaloid;
aralkylamine;
phenylethylamine
Escherichia coli metabolite;
human metabolite;
mouse metabolite
pyrithionepyrithione : A pyridinethione that is pyridine-2(1H)-thione in which the hydrogen attached to the nitrogen is replaced by a hydroxy group. It is a Zn(2+) ionophore; the zinc salt is used as an antifungal and antibacterial agent.

pyrithione: split from cephalosporin molecule; some metal complexes of this have fumarate reductase inhibitory activity and may be useful against trypanosomes; RN given refers to parent cpd; structure
monohydroxypyridine;
pyridinethione
ionophore
aminopropionitrileAminopropionitrile: Reagent used as an intermediate in the manufacture of beta-alanine and pantothenic acid.aminopropionitrileantineoplastic agent;
antirheumatic drug;
collagen cross-linking inhibitor;
plant metabolite
disulfiramorganic disulfide;
organosulfur acaricide
angiogenesis inhibitor;
antineoplastic agent;
apoptosis inducer;
EC 1.2.1.3 [aldehyde dehydrogenase (NAD(+))] inhibitor;
EC 3.1.1.1 (carboxylesterase) inhibitor;
EC 3.1.1.8 (cholinesterase) inhibitor;
EC 5.99.1.2 (DNA topoisomerase) inhibitor;
ferroptosis inducer;
fungicide;
NF-kappaB inhibitor
thiramthiram : An organic disulfide that results from the formal oxidative dimerisation of N,N-dimethyldithiocarbamic acid. It is widely used as a fungicidal seed treatment.

Thiram: A dithiocarbamate chemical, used commercially in the rubber processing industry and as a fungicide. In vivo studies indicate that it inactivates the enzyme GLUTATHIONE REDUCTASE. It has mutagenic activity and may induce chromosomal aberrations.
organic disulfideantibacterial drug;
antifungal agrochemical;
antiseptic drug
benzylamineaminotoluene : Any member of the class of toluenes carrying one or more amino groups.aralkylamine;
primary amine
allergen;
EC 3.5.5.1 (nitrilase) inhibitor;
plant metabolite
4-nitrobenzylamine4-nitrobenzylamine: RN given refers to parent cpd
1-deoxynojirimycin1-deoxy-nojirimycin: structure in first source

duvoglustat : An optically active form of 2-(hydroxymethyl)piperidine-3,4,5-triol having 2R,3R,4R,5S-configuration.
2-(hydroxymethyl)piperidine-3,4,5-triol;
piperidine alkaloid
anti-HIV agent;
anti-obesity agent;
bacterial metabolite;
EC 3.2.1.20 (alpha-glucosidase) inhibitor;
hepatoprotective agent;
hypoglycemic agent;
plant metabolite
miglustatmiglustat : A hydroxypiperidine that is deoxynojirimycin in which the amino hydrogen is replaced by a butyl group.

miglustat: a glucosylceramide synthase inhibitor
piperidines;
tertiary amino compound
anti-HIV agent;
EC 2.4.1.80 (ceramide glucosyltransferase) inhibitor
4-fluorobenzylamine
4-pyridylmethylamine4-pyridylmethylamine: structure given in first source
mor-14N-methyldeoxynojirimycin: glucosidase inhibitorhydroxypiperidine;
piperidine alkaloid;
tertiary amino compound
anti-HIV agent;
cardioprotective agent;
EC 3.2.1.20 (alpha-glucosidase) inhibitor;
plant metabolite
homonojirimycinhomonojirimycin: inhibits alpha-glucosidase; RN given for (2R-(2alpha,3alpha,4beta,5alpha,6beta))-isomer; structure in first source
n-nonyl-1-deoxynojirimycinN-nonyldeoxynojirimycin : A hydroxypiperidine that is deoxynojirimycin (duvoglustat) in which the amino hydrogen is replaced by a nonyl group.hydroxypiperidine;
tertiary amino compound
antiviral agent;
EC 3.2.1.20 (alpha-glucosidase) inhibitor;
EC 3.2.1.45 (glucosylceramidase) inhibitor
somatostatinheterodetic cyclic peptide;
peptide hormone