Histone-lysine N-methyltransferase EHMT2

A histone-lysine N-methyltransferase, H3 lysine-9 specific 3 that is encoded in the genome of human. [PRO:DNx, UniProtKB:Q96KQ7]

Synonyms

EC 2.1.1.-;
Euchromatic histone-lysine N-methyltransferase 2;
HLA-B-associated transcript 8;
Histone H3-K9 methyltransferase 3;
H3-K9-HMTase 3;
Lysine N-methyltransferase 1C;
Protein G9a

Research

Bioassay Publications (31)

Timeframe	Studies on this Protein(%)	All Drugs %
pre-1990	0 (0.00)	18.7374
1990's	0 (0.00)	18.2507
2000's	2 (6.45)	29.6817
2010's	26 (83.87)	24.3611
2020's	3 (9.68)	2.80

Compounds (32)

Drugs with Inhibition Measurements

Drug	Taxonomy	Measurement	Average (mM)	Bioassay(s)	Publication(s)
disulfiram	Homo sapiens (human)	IC50	0.6000	1	1
ebselen	Homo sapiens (human)	IC50	0.4000	1	1
vorinostat	Homo sapiens (human)	IC50	10.0000	1	1
thiram	Homo sapiens (human)	IC50	0.5500	1	1
cystamine dihydrochloride	Homo sapiens (human)	IC50	15.0000	1	1
cysteamine	Homo sapiens (human)	IC50	100.0000	1	1
gliotoxin	Homo sapiens (human)	IC50	3.4650	2	2
9,10-anthraquinone	Homo sapiens (human)	IC50	100.0000	1	1
1,4-naphthoquinone	Homo sapiens (human)	IC50	2.0000	1	1
azacitidine	Homo sapiens (human)	IC50	10.0000	1	1
ninhydrin	Homo sapiens (human)	IC50	54.0000	1	1
diphenyldiselenide	Homo sapiens (human)	IC50	0.5500	1	1
benzeneseleninic acid	Homo sapiens (human)	IC50	4.4000	1	1
sodium selenate	Homo sapiens (human)	IC50	100.0000	1	1
2,2'-dipyridyl disulfide	Homo sapiens (human)	IC50	0.6500	1	1
glutathione disulfide	Homo sapiens (human)	IC50	100.0000	1	1
sinefungin	Homo sapiens (human)	IC50	179.7333	3	3
bisdethiobis(methylthio)gliotoxin	Homo sapiens (human)	IC50	100.0000	1	1
s-adenosylhomocysteine	Homo sapiens (human)	IC50	2.6400	3	3
s-adenosylhomocysteine	Homo sapiens (human)	Ki	0.5700	1	1
decitabine	Homo sapiens (human)	IC50	10.0000	1	1
ditiocarb sodium	Homo sapiens (human)	IC50	9.2000	1	1
verticillins	Homo sapiens (human)	IC50	0.6300	1	1
azodicarbonamide	Homo sapiens (human)	IC50	0.5000	1	1
chetomin	Homo sapiens (human)	IC50	0.1700	1	1
sgi-1027	Homo sapiens (human)	IC50	34.5000	2	2
bix 01294	Homo sapiens (human)	IC50	19.9686	23	23
unc 0638	Homo sapiens (human)	IC50	0.0767	26	26
unc 0638	Homo sapiens (human)	Ki	0.0085	3	3
unc 0321	Homo sapiens (human)	IC50	6.5022	4	4
unc 0631	Homo sapiens (human)	IC50	0.0332	8	8
gsk343	Homo sapiens (human)	IC50	100.0000	1	1
brd4770	Homo sapiens (human)	IC50	6.1000	1	1
6,7-dimethoxy-2-(pyrrolidin-1-yl)-n-(5-(pyrrolidin-1-yl)pentyl)quinazolin-4-amine	Homo sapiens (human)	IC50	100.0000	1	1

Drugs with Activation Measurements

Drug	Taxonomy	Measurement	Average (mM)	Bioassay(s)	Publication(s)
bix 01294	Homo sapiens (human)	Kd	0.1300	1	1
unc 0638	Homo sapiens (human)	Kd	1.4200	1	1
brd4770	Homo sapiens (human)	EC50	5.0000	1	1

Drugs with Other Measurements

Drug	Taxonomy	Measurement	Average (mM)	Bioassay(s)	Publication(s)
unc 0638	Homo sapiens (human)	K	53.6000	1	1

Enables

This protein enables 13 target(s):

Target	Category	Definition
RNA polymerase II transcription regulatory region sequence-specific DNA binding	molecular function	Binding to a specific sequence of DNA that is part of a regulatory region that controls the transcription of a gene or cistron by RNA polymerase II. [GOC:txnOH]
transcription corepressor binding	molecular function	Binding to a transcription corepressor, a protein involved in negative regulation of transcription via protein-protein interactions with transcription factors and other proteins that negatively regulate transcription. Transcription corepressors do not bind DNA directly, but rather mediate protein-protein interactions between repressing transcription factors and the basal transcription machinery. [GOC:krc]
p53 binding	molecular function	Binding to one of the p53 family of proteins. [GOC:hjd]
protein binding	molecular function	Binding to a protein. [GOC:go_curators]
zinc ion binding	molecular function	Binding to a zinc ion (Zn). [GOC:ai]
protein-lysine N-methyltransferase activity	molecular function	Catalysis of the transfer of a methyl group from S-adenosyl-L-methionine to the epsilon-amino group of a lysine residue in a protein substrate. [PMID:12054878]
enzyme binding	molecular function	Binding to an enzyme, a protein with catalytic activity. [GOC:jl]
histone H3K9 methyltransferase activity	molecular function	Catalysis of the reaction: S-adenosyl-L-methionine + histone H3 L-lysine (position 9) = S-adenosyl-L-homocysteine + histone H3 N6-methyl-L-lysine (position 9). This reaction is the addition of up to three methyl groups to the lysine residue at position 9 of the histone H3 protein. [GOC:ai]
histone H3K27 methyltransferase activity	molecular function	Catalysis of the reaction: S-adenosyl-L-methionine + histone H3 L-lysine (position 27) = S-adenosyl-L-homocysteine + histone H3 N6-methyl-L-lysine (position 27). This reaction is the addition of a methyl group to the lysine residue at position 27 of the histone H3 protein. [GOC:ai]
C2H2 zinc finger domain binding	molecular function	Binding to a C2H2-type zinc finger domain of a protein. The C2H2 zinc finger is the classical zinc finger domain, in which two conserved cysteines and histidines co-ordinate a zinc ion. [GOC:BHF, GOC:mah, Pfam:PF00096]
histone H3K56 methyltransferase activity	molecular function	Catalysis of the reaction: S-adenosyl-L-methionine + histone H3 L-lysine (position 56) = S-adenosyl-L-homocysteine + histone H3 N6-methyl-L-lysine (position 56). This reaction is the addition of a methyl group to the lysine residue at position 56 of the histone H3 protein. [PMID:23451023, PMID:28743002]
histone H3K9me2 methyltransferase activity	molecular function	Catalysis of the reaction: N6,N6-dimethyl-L-lysyl9-[histone H3] + S-adenosyl-L-methionine = H+ + N6,N6,N6-trimethyl-L-lysyl9-[histone H3] + S-adenosyl-L-homocysteine. This reaction is the addition of a single methyl group to the dimethylated lysine residue at position 9 of histone H3, producing histone H3K9me3. [RHEA:60280]
promoter-specific chromatin binding	molecular function	Binding to a section of chromatin that is associated with gene promoter sequences of DNA. [PMID:19948729]

Located In

This protein is located in 3 target(s):

Target	Category	Definition
nucleus	cellular component	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. [GOC:go_curators]
nucleoplasm	cellular component	That part of the nuclear content other than the chromosomes or the nucleolus. [GOC:ma, ISBN:0124325653]
nuclear speck	cellular component	A discrete extra-nucleolar subnuclear domain, 20-50 in number, in which splicing factors are seen to be localized by immunofluorescence microscopy. [http://www.cellnucleus.com/]

Active In

This protein is active in 1 target(s):

Target	Category	Definition
nucleus	cellular component	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. [GOC:go_curators]

Part Of

This protein is part of 1 target(s):

Target	Category	Definition
chromatin	cellular component	The ordered and organized complex of DNA, protein, and sometimes RNA, that forms the chromosome. [GOC:elh, PMID:20404130]

Involved In

This protein is involved in 20 target(s):

Target	Category	Definition
regulation of DNA replication	biological process	Any process that modulates the frequency, rate or extent of DNA replication. [GOC:go_curators]
DNA methylation-dependent heterochromatin formation	biological process	Repression of transcription by methylation of DNA, leading to the formation of heterochromatin. [GOC:mah]
synaptonemal complex assembly	biological process	The cell cycle process in which the synaptonemal complex is formed. This is a structure that holds paired chromosomes together during prophase I of meiosis and that promotes genetic recombination. [ISBN:0198506732]
spermatid development	biological process	The process whose specific outcome is the progression of a spermatid over time, from its formation to the mature structure. [GOC:dph, GOC:go_curators]
long-term memory	biological process	The memory process that deals with the storage, retrieval and modification of information a long time (typically weeks, months or years) after receiving that information. This type of memory is typically dependent on gene transcription regulated by second messenger activation. [http://hebb.mit.edu/courses/9.03/lecture4.html, ISBN:0582227089]
fertilization	biological process	The union of gametes of opposite sexes during the process of sexual reproduction to form a zygote. It involves the fusion of the gametic nuclei (karyogamy) and cytoplasm (plasmogamy). [GOC:tb, ISBN:0198506732]
peptidyl-lysine dimethylation	biological process	The methylation of peptidyl-lysine to form peptidyl-N6,N6-dimethyl-L-lysine. [RESID:AA0075]
regulation of protein modification process	biological process	Any process that modulates the frequency, rate or extent of the covalent alteration of one or more amino acid residues within a protein. [GOC:mah, GOC:tb]
organ growth	biological process	The increase in size or mass of an organ. Organs are commonly observed as visibly distinct structures, but may also exist as loosely associated clusters of cells that function together as to perform a specific function. [GOC:bf, ISBN:0471245208, ISBN:0721662544]
phenotypic switching	biological process	A reversible switch of a cell from one cell type or form to another, at a frequency above the expected frequency for somatic mutations. Phenotypic switching involves changes in cell morphology and altered gene expression patterns. For example, Candida albicans switches from white cells to opaque cells for sexual mating. Phenotypic switching also occurs in multicellular organisms; smooth muscle cells (SMCs) exhibit phenotypic transitions to allow rapid adaption to fluctuating environmental cues. [GOC:bf, GOC:di, PMID:12443899, PMID:22406749, PMID:8456504, Wikipedia:Phenotypic_switching]
negative regulation of gene expression via chromosomal CpG island methylation	biological process	An epigenetic gene regulation mechanism that negatively regulates gene expression by methylation of cytosine residues in chromosomal CpG islands. CpG islands are genomic regions that contain a high frequency of the CG dinucleotide associated with the transcription start site of genes. [PMID:11898023, Wikipedia:Cpg_island]
response to ethanol	biological process	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an ethanol stimulus. [GOC:go_curators]
behavioral response to cocaine	biological process	Any process that results in a change in the behavior of an organism as a result of a cocaine stimulus. [GOC:jid]
oocyte development	biological process	The process whose specific outcome is the progression of an oocyte over time, from initial commitment of the cell to its specific fate, to the fully functional differentiated cell. [GOC:go_curators]
neuron fate specification	biological process	The process in which a cell becomes capable of differentiating autonomously into a neuron in an environment that is neutral with respect to the developmental pathway. Upon specification, the cell fate can be reversed. [GOC:dph]
response to fungicide	biological process	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a fungicide stimulus. Fungicides are chemicals used to kill fungi. [GOC:dph]
cellular response to cocaine	biological process	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cocaine stimulus. Cocaine is a crystalline alkaloid obtained from the leaves of the coca plant. [GOC:mah]
cellular response to xenobiotic stimulus	biological process	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus from a xenobiotic, a compound foreign to the organism exposed to it. It may be synthesized by another organism (like ampicilin) or it can be a synthetic chemical. [GOC:krc, GOC:mah]
negative regulation of autophagosome assembly	biological process	Any process that stops, prevents or reduces the frequency, rate or extent of autophagosome assembly. [GO_REF:0000058, GOC:als, GOC:autophagy, GOC:TermGenie, PMID:21975012]
negative regulation of transcription by RNA polymerase II	biological process	Any process that stops, prevents, or reduces the frequency, rate or extent of transcription mediated by RNA polymerase II. [GOC:go_curators, GOC:txnOH]