thymidylate 5'-phosphatase activity
Definition
Target type: molecularfunction
Catalysis of the reaction: thymidylate + H2O = thymidine + phosphate. [EC:3.1.3.35, MetaCyc:THYMIDYLATE-5-PHOSPHATASE-RXN]
Thymidylate 5'-phosphatase activity refers to the enzymatic hydrolysis of the phosphate group from the 5'-position of thymidylate, also known as deoxythymidine monophosphate (dTMP). This reaction is essential for the salvage pathway of nucleotide metabolism, specifically for the recycling of thymine.
The enzyme responsible for this activity, thymidylate 5'-phosphatase, plays a crucial role in maintaining a balanced pool of deoxyribonucleotides, which are the building blocks of DNA. By removing the phosphate group from dTMP, the enzyme converts it into deoxythymidine (dThd). This deoxynucleoside can then be phosphorylated by deoxythymidine kinase to form dTMP again, completing the salvage cycle.
The activity of thymidylate 5'-phosphatase is particularly important for cells that are actively dividing, as they have a high demand for dTMP for DNA synthesis. Defects in this enzyme can lead to impaired DNA replication and cell growth.
The molecular mechanism involves the enzyme binding to dTMP and catalyzing the hydrolysis of the phosphate ester bond using a water molecule. The process releases inorganic phosphate and dThd, which is then available for further processing. The precise catalytic mechanism may vary depending on the specific enzyme, but the overall function remains the same: the removal of the phosphate group from the 5'-position of dTMP.'
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Proteins (1)
| Protein | Definition | Taxonomy |
|---|---|---|
| 5'-nucleotidase | A 5-nucleotidase that is encoded in the genome of human. [PRO:WCB, UniProtKB:P21589] | Homo sapiens (human) |
Compounds (16)
| Compound | Definition | Classes | Roles |
|---|---|---|---|
| sulfamic acid | sulfamic acid : The simplest of the sulfamic acids consisting of a single sulfur atom covalently bound by single bonds to hydroxy and amino groups and by double bonds to two oxygen atoms. sulfamic acid: standard in alkalimetry; RN given refers to parent cpd; structure | sulfamic acids | |
| adenosine monophosphate | Adenosine Monophosphate: Adenine nucleotide containing one phosphate group esterified to the sugar moiety in the 2'-, 3'-, or 5'-position. | adenosine 5'-phosphate; purine ribonucleoside 5'-monophosphate | adenosine A1 receptor agonist; cofactor; EC 3.1.3.1 (alkaline phosphatase) inhibitor; EC 3.1.3.11 (fructose-bisphosphatase) inhibitor; fundamental metabolite; micronutrient; nutraceutical |
| 1-naphthol-8-amino-3,6-disulfonic acid | 1-naphthol-8-amino-3,6-disulfonic acid: dye and found in industrial waste | ||
| 4-sulfanilic acid | 4-aminobenzenesulfonic acid : An aminobenzenesulfonic acid that is aniline sulfonated at the para-position. 4-sulfanilic acid: minor descriptor (75-82); online search SULFANILIC ACIDS; Index Medicus search BENZENESULFONATES | aminobenzenesulfonic acid | allergen; environmental contaminant; xenobiotic; xenobiotic metabolite |
| betulinic acid | hydroxy monocarboxylic acid; pentacyclic triterpenoid | anti-HIV agent; anti-inflammatory agent; antimalarial; antineoplastic agent; EC 5.99.1.3 [DNA topoisomerase (ATP-hydrolysing)] inhibitor; plant metabolite | |
| 1,2,3,4,6-pentakis-O-galloyl-beta-D-glucose | 1,2,3,4,6-pentakis-O-galloyl-beta-D-glucose : A galloyl-beta-D-glucose compound having five galloyl groups in the 1-, 2-, 3-, 4- and 6-positions. pentagalloylglucose: pentahydroxy gallic acid ester of glucose; a phytogenic antineoplastic agent and antibacterial agent | gallate ester; galloyl beta-D-glucose | anti-inflammatory agent; antineoplastic agent; geroprotector; hepatoprotective agent; plant metabolite; radiation protective agent; radical scavenger |
| betulin | betulin : A pentacyclic triterpenoid that is lupane having a double bond at position 20(29) as well as 3beta-hydroxy and 28-hydroxymethyl substituents. betulin: isolated from various white birch bark (BETULA) | diol; pentacyclic triterpenoid | analgesic; anti-inflammatory agent; antineoplastic agent; antiviral agent; metabolite |
| adenosine 5'-methylenediphosphate | nucleoside diphosphate analogue | ||
| Lup-20(29)-en-28-al, 3beta-hydroxy- | triterpenoid | ||
| betulonic acid | betulonic acid: isolated from Rush javanica; strucure in first source | triterpenoid | anticoronaviral agent |
| fludarabine | purine nucleoside | ||
| ellagic acid | catechols; cyclic ketone; lactone; organic heterotetracyclic compound; polyphenol | antioxidant; EC 1.14.18.1 (tyrosinase) inhibitor; EC 2.3.1.5 (arylamine N-acetyltransferase) inhibitor; EC 2.4.1.1 (glycogen phosphorylase) inhibitor; EC 2.5.1.18 (glutathione transferase) inhibitor; EC 2.7.1.127 (inositol-trisphosphate 3-kinase) inhibitor; EC 2.7.1.151 (inositol-polyphosphate multikinase) inhibitor; EC 2.7.4.6 (nucleoside-diphosphate kinase) inhibitor; EC 2.7.7.7 (DNA-directed DNA polymerase) inhibitor; EC 5.99.1.2 (DNA topoisomerase) inhibitor; EC 5.99.1.3 [DNA topoisomerase (ATP-hydrolysing)] inhibitor; food additive; fungal metabolite; geroprotector; plant metabolite; skin lightening agent | |
| 3-nitrobenzaldehyde isonicotinoylhydrazone | 3-nitrobenzaldehyde isonicotinoylhydrazone: structure in first source | ||
| 23-hydroxybetulinic acid | 23-hydroxybetulinic acid: structure in first source | triterpenoid | metabolite |
| salinazid | aromatic carboxylic acid; pyridinemonocarboxylic acid | ||
| phthivazide |