5'-nucleotidase

A 5-nucleotidase that is encoded in the genome of human. [PRO:WCB, UniProtKB:P21589]

Synonyms

5'-NT;
EC 3.1.3.5;
Ecto-5'-nucleotidase

Research

Bioassay Publications (14)

Timeframe	Studies on this Protein(%)	All Drugs %
pre-1990	0 (0.00)	18.7374
1990's	0 (0.00)	18.2507
2000's	1 (7.14)	29.6817
2010's	5 (35.71)	24.3611
2020's	8 (57.14)	2.80

Compounds (16)

Drugs with Inhibition Measurements

Drug	Taxonomy	Measurement	Average (mM)	Bioassay(s)	Publication(s)
sulfamic acid	Homo sapiens (human)	IC50	56.1800	3	5
1-naphthol-8-amino-3,6-disulfonic acid	Homo sapiens (human)	IC50	21.8000	1	1
4-sulfanilic acid	Homo sapiens (human)	IC50	8.2000	1	1
betulinic acid	Homo sapiens (human)	IC50	7.2800	1	1
1,2,3,4,6-pentakis-O-galloyl-beta-D-glucose	Homo sapiens (human)	IC50	10.5400	1	1
betulin	Homo sapiens (human)	IC50	22.6600	2	2
adenosine 5'-methylenediphosphate	Homo sapiens (human)	IC50	5.7536	11	11
adenosine 5'-methylenediphosphate	Homo sapiens (human)	Ki	0.1656	7	7
Lup-20(29)-en-28-al, 3beta-hydroxy-	Homo sapiens (human)	IC50	100.0000	1	1
betulonic acid	Homo sapiens (human)	IC50	43.2600	1	1
fludarabine	Homo sapiens (human)	Ki	700.0000	2	2
ellagic acid	Homo sapiens (human)	IC50	1.8500	1	1
3-nitrobenzaldehyde isonicotinoylhydrazone	Homo sapiens (human)	IC50	1.0550	1	2
23-hydroxybetulinic acid	Homo sapiens (human)	IC50	100.0000	1	1
salinazid	Homo sapiens (human)	IC50	0.8950	1	2
phthivazide	Homo sapiens (human)	IC50	0.4100	1	2

Drugs with Activation Measurements

Drug	Taxonomy	Measurement	Average (mM)	Bioassay(s)	Publication(s)
adenosine 5'-methylenediphosphate	Homo sapiens (human)	EC50	16.2680	1	1

Drugs with Other Measurements

Drug	Taxonomy	Measurement	Average (mM)	Bioassay(s)	Publication(s)
adenosine monophosphate	Homo sapiens (human)	Km	14.0000	1	1

Enables

This protein enables 10 target(s):

Target	Category	Definition
nucleotide binding	molecular function	Binding to a nucleotide, any compound consisting of a nucleoside that is esterified with (ortho)phosphate or an oligophosphate at any hydroxyl group on the ribose or deoxyribose. [GOC:mah, ISBN:0198547684]
5'-deoxynucleotidase activity	molecular function	Catalysis of the reaction:a 2'-deoxyribonucleoside 5'-monophosphate + H20=a 2'-deoxyribonucleoside + phosphate. [EC:3.1.3.89]
protein binding	molecular function	Binding to a protein. [GOC:go_curators]
5'-nucleotidase activity	molecular function	Catalysis of the reaction: a 5'-ribonucleotide + H2O = a ribonucleoside + phosphate. [EC:3.1.3.5]
zinc ion binding	molecular function	Binding to a zinc ion (Zn). [GOC:ai]
identical protein binding	molecular function	Binding to an identical protein or proteins. [GOC:jl]
thymidylate 5'-phosphatase activity	molecular function	Catalysis of the reaction: thymidylate + H2O = thymidine + phosphate. [EC:3.1.3.35, MetaCyc:THYMIDYLATE-5-PHOSPHATASE-RXN]
IMP 5'-nucleotidase activity	molecular function	Catalysis of the reaction: 5'-IMP + H2O = inosine + phosphate. [GOC:ai]
GMP 5'-nucleotidase activity	molecular function	Catalysis of the reaction: 5'-GMP + H2O = guanosine + phosphate. [GOC:ai]
XMP 5'-nucleosidase activity	molecular function	Catalysis of the reaction: 5'XMP + H20 = phosphate + xanthosine. [PMID:34824243, RHEA:28530]

Located In

This protein is located in 7 target(s):

Target	Category	Definition
nucleoplasm	cellular component	That part of the nuclear content other than the chromosomes or the nucleolus. [GOC:ma, ISBN:0124325653]
cytosol	cellular component	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. [GOC:hjd, GOC:jl]
plasma membrane	cellular component	The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. [ISBN:0716731363]
external side of plasma membrane	cellular component	The leaflet of the plasma membrane that faces away from the cytoplasm and any proteins embedded or anchored in it or attached to its surface. [GOC:dos, GOC:tb]
cell surface	cellular component	The external part of the cell wall and/or plasma membrane. [GOC:jl, GOC:mtg_sensu, GOC:sm]
membrane	cellular component	A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. [GOC:dos, GOC:mah, ISBN:0815316194]
extracellular exosome	cellular component	A vesicle that is released into the extracellular region by fusion of the limiting endosomal membrane of a multivesicular body with the plasma membrane. Extracellular exosomes, also simply called exosomes, have a diameter of about 40-100 nm. [GOC:BHF, GOC:mah, GOC:vesicles, PMID:15908444, PMID:17641064, PMID:19442504, PMID:19498381, PMID:22418571, PMID:24009894]

Active In

This protein is active in 1 target(s):

Target	Category	Definition
plasma membrane	cellular component	The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. [ISBN:0716731363]

Involved In

This protein is involved in 11 target(s):

Target	Category	Definition
DNA metabolic process	biological process	Any cellular metabolic process involving deoxyribonucleic acid. This is one of the two main types of nucleic acid, consisting of a long, unbranched macromolecule formed from one, or more commonly, two, strands of linked deoxyribonucleotides. [ISBN:0198506732]
leukocyte cell-cell adhesion	biological process	The attachment of a leukocyte to another cell via adhesion molecules. [GOC:go_curators]
response to inorganic substance	biological process	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an inorganic substance stimulus. [GOC:sm]
response to ATP	biological process	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an ATP (adenosine 5'-triphosphate) stimulus. [GOC:sl]
ADP catabolic process	biological process	The chemical reactions and pathways resulting in the breakdown of ADP, adenosine 5'-diphosphate. [GOC:go_curators]
ATP metabolic process	biological process	The chemical reactions and pathways involving ATP, adenosine triphosphate, a universally important coenzyme and enzyme regulator. [GOC:go_curators]
adenosine biosynthetic process	biological process	The chemical reactions and pathways resulting in the formation of adenosine, adenine riboside, a ribonucleoside found widely distributed in cells of every type as the free nucleoside and in combination in nucleic acids and various nucleoside coenzymes. [GOC:go_curators]
negative regulation of inflammatory response	biological process	Any process that stops, prevents, or reduces the frequency, rate or extent of the inflammatory response. [GOC:ai]
calcium ion homeostasis	biological process	Any process involved in the maintenance of an internal steady state of calcium ions within an organism or cell. [GOC:ceb, GOC:jid, GOC:mah]
inhibition of non-skeletal tissue mineralization	biological process	A homeostatic process involved in the maintenance of non-mineral tissue, by preventing mineralization of non-skeletal tissue. [PMID:21490328, PMID:30030150]
AMP catabolic process	biological process	The chemical reactions and pathways resulting in the breakdown of AMP, adenosine monophosphate. [ISBN:0198506732]