Target type: molecularfunction
Catalysis of the transfer of a glycosyl group from one compound (donor) to another (acceptor). [GOC:jl, ISBN:0198506732]
Glycosyltransferase activity is a fundamental molecular function in the biological world, crucial for the synthesis of a diverse array of complex carbohydrates. Glycosyltransferases (GTs) are enzymes that catalyze the transfer of a sugar moiety (glycosyl residue) from a donor molecule, often a sugar nucleotide like UDP-glucose or GDP-mannose, to an acceptor molecule, which can be a lipid, protein, or another carbohydrate. This transfer process involves the formation of a glycosidic bond, linking the sugar to the acceptor. The diversity of glycosyltransferases and their specificities for both donor and acceptor molecules contribute to the remarkable structural diversity of glycans, ranging from short oligosaccharides to highly branched polysaccharides. Glycosyltransferases play essential roles in numerous biological processes, including:
- **Cellular recognition and signaling:** Glycosylation patterns on cell surfaces are crucial for cell-cell interactions, immune recognition, and signal transduction pathways.
- **Protein folding and stability:** Glycosylation of proteins can influence their folding, stability, and function.
- **Extracellular matrix assembly:** Glycosyltransferases are involved in the synthesis of complex carbohydrates that form the extracellular matrix, providing structural support and regulating cell adhesion.
- **Cellular metabolism and homeostasis:** Glycosylation is involved in the synthesis of important metabolic intermediates, such as glycogen and starch.
- **Development and differentiation:** Glycosylation plays a role in cell differentiation, developmental processes, and tissue morphogenesis.
The molecular mechanism of glycosyltransferase activity involves several key steps:
1. **Substrate binding:** The GT binds both the donor and acceptor molecules in its active site.
2. **Sugar activation:** The donor sugar nucleotide is activated by the enzyme, often through a conformational change.
3. **Glycosyl transfer:** The activated sugar moiety is transferred from the donor to the acceptor molecule, forming a new glycosidic bond.
4. **Product release:** The enzyme releases the newly synthesized glycoconjugate and is ready for another catalytic cycle.
The specificities of glycosyltransferases are determined by their amino acid sequences, which dictate their active sites and substrate preferences. This remarkable specificity allows for the precise control of glycosylation patterns and the creation of highly complex glycans. Mutations in glycosyltransferases can lead to a variety of genetic disorders, underscoring their importance in human health. Therefore, understanding glycosyltransferase activity is crucial for comprehending fundamental biological processes and developing new therapeutic strategies.'
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Protein | Definition | Taxonomy |
---|---|---|
Lipopolysaccharide heptosyltransferase 1 | A lipopolysaccharide heptosyltransferase 1 that is encoded in the genome of Escherichia coli K-12. [PRO:DNx, UniProtKB:P24173] | Escherichia coli K-12 |
Thymidine phosphorylase | A thymidine phosphorylase that is encoded in the genome of Escherichia coli K-12. [OMA:P07650, PRO:DNx] | Escherichia coli K-12 |
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase | A UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase that is encoded in the genome of human. [PRO:DNx, UniProtKB:Q9H3H5] | Homo sapiens (human) |
Compound | Definition | Classes | Roles |
---|---|---|---|
thymidine | pyrimidine 2'-deoxyribonucleoside | Escherichia coli metabolite; human metabolite; metabolite; mouse metabolite | |
galactose | galactopyranose : The pyranose form of galactose. | D-galactose; galactopyranose | Escherichia coli metabolite; mouse metabolite |
methylglucoside, (alpha-d)-isomer | methyl alpha-D-glucopyranoside : An alpha-D-glucopyranoside having a methyl substituent at the anomeric position. | alpha-D-glucoside; methyl D-glucoside | |
2',3'-dideoxythymidine | |||
6-amino-5-bromouracil | |||
5-nitro-2'-deoxyuridine | |||
5-phenyl-1,3,4-oxadiazole-2-thiol | 5-phenyl-1,3,4-oxadiazole-2-thiol: structure in first source | ||
capuramycin | capuramycin: from Streptomyces griseus 446-S3; structure given in first source | ||
ma-1 | tipiracil : A member of the class of pyrimidones that is uracil substituted by chloro and (2-iminopyrrolidin-1-yl)methyl groups at positions 5 and 6 respectively. Used (as the hydrochloride salt) in combination with trifluridine, a nucleoside metabolic inhibitor, for treatment of advanced/relapsed unresectable colorectal cancer. tipiracil: inhibits thymidine phosphorylase | carboxamidine; organochlorine compound; pyrimidone; pyrrolidines | antineoplastic agent; EC 2.4.2.4 (thymidine phosphorylase) inhibitor |
5-chloro-6-(1-(2-iminopyrrolidinyl) methyl)uracil hydrochloride | tipiracil hydrochloride : A hydrochloride obtained by combining tipiracil with one equivalent of hydrochloric acid. Used in combination with trifluridine, a nucleoside metabolic inhibitor, for treatment of advanced/relapsed unresectable colorectal cancer. | hydrochloride; iminium salt | antineoplastic agent; EC 2.4.2.4 (thymidine phosphorylase) inhibitor |
sesone | 7-deazaxanthine: structure in first source | ||
5'-o-tritylinosine | 5'-O-tritylinosine: structure in first source |