Page last updated: 2024-08-07 15:36:58
Thymidine kinase, cytosolic
A thymidine kinase, cytosolic that is encoded in the genome of human. [PRO:DNx, UniProtKB:P04183]
Synonyms
EC 2.7.1.21
Research
Bioassay Publications (16)
| Timeframe | Studies on this Protein(%) | All Drugs % |
|---|---|---|
| pre-1990 | 3 (18.75) | 18.7374 |
| 1990's | 3 (18.75) | 18.2507 |
| 2000's | 8 (50.00) | 29.6817 |
| 2010's | 1 (6.25) | 24.3611 |
| 2020's | 1 (6.25) | 2.80 |
Compounds (28)
Drugs with Inhibition Measurements
| Drug | Taxonomy | Measurement | Average (mM) | Bioassay(s) | Publication(s) |
|---|---|---|---|---|---|
| thymidine | Homo sapiens (human) | IC50 | 125.4605 | 4 | 4 |
| thymidine | Homo sapiens (human) | Ki | 1.1000 | 2 | 2 |
| idoxuridine | Homo sapiens (human) | Ki | 0.0900 | 1 | 1 |
| bromodeoxyuridine | Homo sapiens (human) | Ki | 15.0300 | 2 | 2 |
| cytarabine | Homo sapiens (human) | IC50 | 1,000.0000 | 2 | 2 |
| stavudine | Homo sapiens (human) | Ki | 597.5000 | 2 | 2 |
| zidovudine | Homo sapiens (human) | IC50 | 3.0000 | 1 | 1 |
| zidovudine | Homo sapiens (human) | Ki | 4.9500 | 2 | 2 |
| fialuridine | Homo sapiens (human) | Ki | 0.5450 | 2 | 2 |
| thymine arabinoside | Homo sapiens (human) | IC50 | 1,000.0000 | 2 | 2 |
| 2',3'-didehydro-2',3'-dideoxyuridine | Homo sapiens (human) | Ki | 445.0000 | 1 | 1 |
| clevudine | Homo sapiens (human) | Ki | 0.7900 | 2 | 2 |
| 5-propyl-2'-deoxyuridine | Homo sapiens (human) | Ki | 20.0000 | 1 | 1 |
| 5'-o-dimethyltritylthymidine | Homo sapiens (human) | IC50 | 14.0000 | 1 | 1 |
| 1-(2-deoxy-2-fluoroarabinofuranosyl)-5-ethyluracil | Homo sapiens (human) | Ki | 0.2650 | 2 | 2 |
| (north)-methanocarbathymidine | Homo sapiens (human) | Ki | 16.1000 | 1 | 1 |
| brivudine | Homo sapiens (human) | Ki | 0.1000 | 1 | 1 |
| carbocyclic 5-iodo-2'-deoxyuridine | Homo sapiens (human) | Ki | 0.2100 | 1 | 1 |
| deoxy-4-thiothymidine | Homo sapiens (human) | Ki | 12.0000 | 1 | 1 |
| sorivudine | Homo sapiens (human) | IC50 | 500.0000 | 1 | 1 |
| deoxyguanosine | Homo sapiens (human) | IC50 | 239.5000 | 2 | 2 |
| 9-(4-hydroxybutyl)guanine | Homo sapiens (human) | Ki | 2.0600 | 1 | 1 |
| 9-arabinofuranosylguanine | Homo sapiens (human) | IC50 | 500.0000 | 1 | 1 |
Drugs with Other Measurements
| Drug | Taxonomy | Measurement | Average (mM) | Bioassay(s) | Publication(s) |
|---|---|---|---|---|---|
| thymidine | Homo sapiens (human) | ID50 | 2.0000 | 1 | 1 |
| thymidine | Homo sapiens (human) | Km | 7.0611 | 9 | 9 |
| thymidine monophosphate | Homo sapiens (human) | Km | 5.0000 | 1 | 1 |
| deoxyuridine | Homo sapiens (human) | ID50 | 400.0000 | 1 | 1 |
| 2'-deoxyadenosine | Homo sapiens (human) | ID50 | 1,000.0000 | 1 | 1 |
| stavudine | Homo sapiens (human) | Km | 133.0000 | 1 | 1 |
| vidarabine | Homo sapiens (human) | Km | 1.5000 | 1 | 1 |
| zidovudine | Homo sapiens (human) | Km | 3.1333 | 3 | 3 |
| fialuridine | Homo sapiens (human) | Km | 53.0000 | 1 | 1 |
| 3'-azido-3'-deoxythymidine 5'phosphate | Homo sapiens (human) | Km | 12.0000 | 1 | 1 |
| clevudine | Homo sapiens (human) | Km | 60.0000 | 1 | 1 |
| 1-(2-deoxy-2-fluoroarabinofuranosyl)-5-ethyluracil | Homo sapiens (human) | Km | 3,154.7000 | 1 | 1 |
| 2',3'-didehydro-3'-deoxy-4'-ethynylthymidine | Homo sapiens (human) | Km | 53.0000 | 1 | 1 |
| acyclovir | Homo sapiens (human) | Km | 1.8500 | 2 | 2 |
| deoxyguanosine | Homo sapiens (human) | ID50 | 300.0000 | 1 | 1 |
Enables
This protein enables 5 target(s):
| Target | Category | Definition |
|---|---|---|
| thymidine kinase activity | molecular function | Catalysis of the reaction: ATP + thymidine = ADP + thymidine 5'-phosphate. [EC:2.7.1.21] |
| protein binding | molecular function | Binding to a protein. [GOC:go_curators] |
| ATP binding | molecular function | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. [ISBN:0198506732] |
| zinc ion binding | molecular function | Binding to a zinc ion (Zn). [GOC:ai] |
| identical protein binding | molecular function | Binding to an identical protein or proteins. [GOC:jl] |
Located In
This protein is located in 2 target(s):
| Target | Category | Definition |
|---|---|---|
| nucleus | cellular component | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. [GOC:go_curators] |
| cytosol | cellular component | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. [GOC:hjd, GOC:jl] |
Involved In
This protein is involved in 6 target(s):
| Target | Category | Definition |
|---|---|---|
| nucleobase-containing compound metabolic process | biological process | Any cellular metabolic process involving nucleobases, nucleosides, nucleotides and nucleic acids. [GOC:ai] |
| deoxyribonucleoside monophosphate biosynthetic process | biological process | The chemical reactions and pathways resulting in the formation of a deoxyribonucleoside monophosphate, a compound consisting of a nucleobase linked to a deoxyribose sugar esterified with phosphate on the sugar. [GOC:go_curators, ISBN:0198506732] |
| thymidine metabolic process | biological process | The chemical reactions and pathways involving thymidine, deoxyribosylthymine thymine 2-deoxyriboside, a deoxynucleoside very widely distributed but occurring almost entirely as phosphoric esters in deoxynucleotides and deoxyribonucleic acid, DNA. [GOC:go_curators] |
| thymidine biosynthetic process | biological process | The chemical reactions and pathways resulting in the formation of thymidine, deoxyribosylthymine thymine 2-deoxyriboside, a deoxynucleoside very widely distributed but occurring almost entirely as phosphoric esters in deoxynucleotides and deoxyribonucleic acid, DNA. [GOC:go_curators] |
| protein homotetramerization | biological process | The formation of a protein homotetramer, a macromolecular structure consisting of four noncovalently associated identical subunits. [GOC:go_curators] |
| DNA synthesis involved in mitotic DNA replication | biological process | Any DNA biosynthetic process that is involved in mitotic DNA replication. [GO_REF:0000060, GOC:TermGenie, PMID:16849602] |