Page last updated: 2024-10-24

N-acylphosphatidylethanolamine-specific phospholipase D activity

Definition

Target type: molecularfunction

Catalysis of the reaction: H2O + N-acyl-1,2-diacyl-sn-glycero-3-phosphoethanolamine (NAPE) = a 1,2-diacyl-sn-glycero-3-phosphate + an N-acylethanolamine (NAE) + H+. [PMID:14634025, PMID:15878693, RHEA:33159]

N-acylphosphatidylethanolamine-specific phospholipase D (NAPE-PLD) activity is a crucial enzymatic process involved in the hydrolysis of N-acylphosphatidylethanolamine (NAPE), a membrane phospholipid. NAPE-PLD specifically cleaves the phosphodiester bond between the phosphate group and the diacylglycerol moiety of NAPE, releasing N-acylethanolamine (NAE) and phosphatidic acid (PA). NAE, also known as anandamide, is a well-characterized endocannabinoid neurotransmitter that plays a significant role in regulating various physiological functions, including pain perception, appetite, and memory. PA, on the other hand, acts as a signaling molecule involved in diverse cellular processes, including membrane trafficking, cell growth, and cytoskeletal organization. NAPE-PLD activity is tightly regulated and influenced by various factors, such as calcium concentration, protein phosphorylation, and interactions with other proteins. This enzymatic activity is essential for the production of NAE, a critical mediator of endocannabinoid signaling, and for the generation of PA, a versatile signaling molecule involved in numerous cellular pathways. The precise regulation and activity of NAPE-PLD are crucial for maintaining cellular homeostasis and for mediating physiological responses to various stimuli.'
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Proteins (4)

ProteinDefinitionTaxonomy
N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase DAn N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D that is encoded in the genome of human. [PRO:DNx, UniProtKB:Q6IQ20]Homo sapiens (human)
N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase DAn N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D that is encoded in the genome of human. [PRO:DNx, UniProtKB:Q6IQ20]Homo sapiens (human)
Phospholipase D1A phospholipase D1 that is encoded in the genome of human. [PRO:DNx, UniProtKB:Q13393]Homo sapiens (human)
Phospholipase D2A phospholipase D2 that is encoded in the genome of human. [PRO:DNx, UniProtKB:O14939]Homo sapiens (human)

Compounds (16)

CompoundDefinitionClassesRoles
hexachlorophenehexachlorophene : An organochlorine compound that is diphenylmethane in which each of the phenyl groups is substituted by chlorines at positions 2, 3, and 5, and by a hydroxy group at position 6. An antiseptic that is effective against Gram-positive organisms, it is used in soaps and creams for the treatment of various skin disorders. It is also used in agriculture as an acaricide and fungicide, but is not approved for such use within the European Union.

Hexachlorophene: A chlorinated bisphenol antiseptic with a bacteriostatic action against Gram-positive organisms, but much less effective against Gram-negative organisms. It is mainly used in soaps and creams and is an ingredient of various preparations used for skin disorders. (From Martindale, The Extra Pharmacopoeia, 30th ed, p797)
bridged diphenyl fungicide;
polyphenol;
trichlorobenzene
acaricide;
antibacterial agent;
antifungal agrochemical;
antiseptic drug
1-NA-PP1pyrazolopyrimidinetyrosine kinase inhibitor
raloxifeneraloxifene : A member of the class of 1-benzothiophenes that is 1-benzothiophene in which the hydrogens at positions 2, 3, and 6 have been replaced by p-hydroxyphenyl, p-[2-(piperidin-1-yl)ethoxy]benzoyl, and hydroxy groups, respectively.1-benzothiophenes;
aromatic ketone;
N-oxyethylpiperidine;
phenols
bone density conservation agent;
estrogen antagonist;
estrogen receptor modulator
lithocholic acidlithocholate : A bile acid anion that is the conjugate base of lithocholic acid.

lithocholic acid : A monohydroxy-5beta-cholanic acid with a alpha-hydroxy substituent at position 3. It is a bile acid obtained from chenodeoxycholic acid by bacterial action.

Lithocholic Acid: A bile acid formed from chenodeoxycholate by bacterial action, usually conjugated with glycine or taurine. It acts as a detergent to solubilize fats for absorption and is itself absorbed. It is used as cholagogue and choleretic.
bile acid;
C24-steroid;
monohydroxy-5beta-cholanic acid
geroprotector;
human metabolite;
mouse metabolite
4-[1-[4-[2-(dimethylamino)ethoxy]phenyl]-2-phenylbut-1-enyl]phenolstilbenoid
halopemidehalopemide: structure
1-(4-pyridyl)piperazine1-(4-pyridyl)piperazine: structure in first source
1-(2-pyridinyl)piperazine1-(2-pyridinyl)piperazine: metabolite of buspirone & gepirone
afimoxifeneafimoxifene : A tertiary amino compound that is tamoxifen in which the phenyl group which is in a Z- relationship to the ethyl substituent is hydroxylated at the para- position. It is the active metabolite of tamoxifen.phenols;
tertiary amino compound
antineoplastic agent;
estrogen receptor antagonist;
metabolite
tamoxifenstilbenoid;
tertiary amino compound
angiogenesis inhibitor;
antineoplastic agent;
bone density conservation agent;
EC 1.2.3.1 (aldehyde oxidase) inhibitor;
EC 2.7.11.13 (protein kinase C) inhibitor;
estrogen antagonist;
estrogen receptor antagonist;
estrogen receptor modulator
nitrocefinnitrocefin: chromogenic cephalosporin used for detection of beta-lactamase activity; Cefinase is name for nitrocefin on paper disc; RN given refers to (6R-(3(E),6 alpha,7 beta))-isomer; structure for mono-Na salt in second source
5-fluoro-2-indolyldeschlorohalopemidebenzimidazoles
N-[2-[4-(2-oxo-3H-benzimidazol-1-yl)-1-piperidinyl]ethyl]-2-naphthalenecarboxamidenaphthalenecarboxamide
vu0155069
ml298
ml299ML299: has antineoplastic activity; structure in first source