Gene Targets > glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity
Page last updated: 2024-10-24

glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity

Definition

Target type: molecularfunction

Catalysis of the reaction: D-glyceraldehyde 3-phosphate + NAD+ + H2O = 3-phospho-D-glycerate + NADH + H+. [PMID:9497334]

Glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity catalyzes the reversible oxidation of glyceraldehyde 3-phosphate to 3-phosphoglycerate using NAD+ as an electron acceptor. This reaction is a key step in glycolysis, a metabolic pathway that breaks down glucose to produce ATP. In this non-phosphorylating form, the enzyme does not utilize inorganic phosphate to generate 1,3-bisphosphoglycerate. Instead, it uses NAD+ to oxidize glyceraldehyde 3-phosphate, generating NADH and 3-phosphoglycerate. This reaction is distinct from the canonical glyceraldehyde-3-phosphate dehydrogenase activity, which is phosphorylating and results in the production of 1,3-bisphosphoglycerate. The non-phosphorylating activity is often found in organisms that lack or have reduced levels of the phosphorylating activity. It is also important in certain metabolic pathways, such as the pentose phosphate pathway, where it plays a role in the conversion of glucose 6-phosphate to ribose 5-phosphate.'
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Proteins (3)

ProteinDefinitionTaxonomy
Aldehyde dehydrogenase X, mitochondrialAn aldehyde dehydrogenase X, mitochondrial that is encoded in the genome of human. [PRO:DNx, UniProtKB:P30837]Homo sapiens (human)
Aldehyde dehydrogenase, mitochondrialAn aldehyde dehydrogenase, mitochondrial that is encoded in the genome of human. [PRO:DNx, UniProtKB:P05091]Homo sapiens (human)
Retinal dehydrogenase 1An aldehyde dehydrogenase 1A1 that is encoded in the genome of human. [PRO:DNx, UniProtKB:P00352]Homo sapiens (human)

Compounds (18)

CompoundDefinitionClassesRoles
disulfiramorganic disulfide;
organosulfur acaricide		angiogenesis inhibitor;
antineoplastic agent;
apoptosis inducer;
EC 1.2.1.3 [aldehyde dehydrogenase (NAD(+))] inhibitor;
EC 3.1.1.1 (carboxylesterase) inhibitor;
EC 3.1.1.8 (cholinesterase) inhibitor;
EC 5.99.1.2 (DNA topoisomerase) inhibitor;
ferroptosis inducer;
fungicide;
NF-kappaB inhibitor
perphenazineperphenazine : A phenothiazine derivative in which the phenothiazine tricycle carries a chloro substituent at the 2-position and a 3-[4-(2-hydroxyethyl)piperazin-1-yl]propyl group at N-10.

Perphenazine: An antipsychotic phenothiazine derivative with actions and uses similar to those of CHLORPROMAZINE.		N-(2-hydroxyethyl)piperazine;
N-alkylpiperazine;
organochlorine compound;
phenothiazines		antiemetic;
dopaminergic antagonist;
phenothiazine antipsychotic drug
thiramthiram : An organic disulfide that results from the formal oxidative dimerisation of N,N-dimethyldithiocarbamic acid. It is widely used as a fungicidal seed treatment.

Thiram: A dithiocarbamate chemical, used commercially in the rubber processing industry and as a fungicide. In vivo studies indicate that it inactivates the enzyme GLUTATHIONE REDUCTASE. It has mutagenic activity and may induce chromosomal aberrations.		organic disulfideantibacterial drug;
antifungal agrochemical;
antiseptic drug
cystamine dihydrochloride
uvitex swnUvitex SWN: optical brightner from Ciba; structure7-aminocoumarinsfluorochrome
bis(1-piperidylthiocarbonyl)disulfidebis(1-piperidylthiocarbonyl)disulfide: indicator for analysis of copper; structure
win 18446WIN 18446 : A carboxamide that is 1,8-diaminooctane in which a hydrogen attached to each of the amino groups has been replaced by a dichloroacetyl group. Inhibitor of aldehyde dehydrogenase 1a2 (ALDH1a2). Inhibits the biosynthesis of retinoic acid from retinol in neonatal and adult murine testis. It down-regulates sex related genes in zebrafish.organochlorine compound;
secondary carboxamide		EC 1.2.1.3 [aldehyde dehydrogenase (NAD(+))] inhibitor
4-(diethylamino)benzaldehyde4-(diethylamino)benzaldehyde : A member of the class of benzaldehydes carrying a diethylamino substituent at position 4.aromatic amine;
benzaldehydes;
tertiary amino compound		EC 1.2.1.3 [aldehyde dehydrogenase (NAD(+))] inhibitor
magnololbiphenyls
daidzindaidzein 7-O-beta-D-glucoside : A glycosyloxyisoflavone that is daidzein attached to a beta-D-glucopyranosyl residue at position 7 via a glycosidic linkage. It is used in the treatment of alcohol dependency (antidipsotropic).

daidzin: a potent, selective, and reversible inhibitor of human mitochondrial aldehyde dehydrogenase		7-hydroxyisoflavones 7-O-beta-D-glucoside;
hydroxyisoflavone;
monosaccharide derivative		plant metabolite
ononin4'-methoxyisoflavones;
7-hydroxyisoflavones 7-O-beta-D-glucoside;
monosaccharide derivative		plant metabolite
7-butyl-8-[[4-(3-chlorophenyl)-1-piperazinyl]methyl]-1,3-dimethylpurine-2,6-dionepiperazines
n-(1,3-benzodioxol-5-ylmethyl)-2,6-dichlorobenzamideN-(1,3-benzodioxol-5-ylmethyl)-2,6-dichlorobenzamide: Cardioprotective Agent; a small-molecule activator of aldehyde dehydrogenase-2 that reduces ischemic damage to the heartcarbonyl compound;
organohalogen compound
daidzein7-hydroxyisoflavonesantineoplastic agent;
EC 2.7.7.7 (DNA-directed DNA polymerase) inhibitor;
EC 3.2.1.20 (alpha-glucosidase) inhibitor;
phytoestrogen;
plant metabolite
prunetinprunetin : A hydroxyisoflavone that is genistein in which the hydroxy group at position 7 is replaced by a methoxy group.

prunetin: reduces herpes virus-1 plaque formation		7-methoxyisoflavones;
hydroxyisoflavone		anti-inflammatory agent;
EC 1.2.1.3 [aldehyde dehydrogenase (NAD(+))] inhibitor;
EC 1.3.1.22 [3-oxo-5alpha-steroid 4-dehydrogenase (NADP(+))] inhibitor;
metabolite
4'-hydroxychalcone4'-hydroxychalcone : A member of the class of chalcones that is trans-chalcone substituted by a hydroxy group at position 4'.

4'-hydroxychalcone: inhibits TNFalpha-induced NF-κB activation; structure in first source		chalcones;
phenols		anti-inflammatory agent;
antineoplastic agent
2-acetylfuranonaphthoquinone2-acetylfuranonaphthoquinone: has antineoplastic activity; structure in first source
nct-501NCT-501: inhibits aldehyde dehydrogenase 1A1; structure in first source