4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase activity

Definition

Target type: molecularfunction

Catalysis of the reaction: GDP-beta-L-fucose + beta-D-galactosyl-(1,4)-N-acetyl-D-glucosaminyl-R = GDP + 1,4-beta-D-galactosyl-(1,4)-[alpha-L-fucosyl-(1,3)]-N-acetyl-D-glucosaminyl-R. [EC:2.4.1.152, RHEA:14257]

4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase activity is a type of glycosyltransferase that catalyzes the transfer of a fucose residue from a GDP-fucose donor to the 3-alpha position of N-acetylglucosamine (GlcNAc) that is linked to galactose in a complex N-glycan. This specific transfer reaction is critical for the biosynthesis of various complex N-glycans, which are crucial for proper protein folding, stability, and cellular function.

The enzyme's activity is highly specific for both the donor substrate (GDP-fucose) and the acceptor substrate (the N-glycan containing GlcNAc linked to galactose). The reaction occurs in the Golgi apparatus, where the N-glycans are processed and modified.

The 3-alpha-L-fucosylation catalyzed by this enzyme is important for several reasons:

* **Protein folding and stability:** The addition of fucose residues to N-glycans can influence protein folding and stability. This is particularly important for proteins that are destined for secretion or for the cell surface.
* **Cell-cell interactions:** Fucosylated N-glycans play a role in cell-cell interactions, such as cell adhesion and signaling.
* **Immune response:** Fucosylation can affect the recognition of proteins by the immune system. For example, the presence of fucose residues on certain glycoproteins can inhibit their interaction with antibodies.

Overall, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase activity is an essential enzyme involved in the biosynthesis of complex N-glycans, which are crucial for a variety of biological processes.'
"

Proteins (4)

Compounds (12)