Page last updated: 2024-10-24

RNA cap binding

Definition

Target type: molecularfunction

Binding to a 7-methylguanosine (m7G) group or derivative located at the 5' end of an RNA molecule. [GOC:krc]

RNA cap binding is a fundamental molecular function in eukaryotic gene expression. It involves the specific recognition and binding of the 5’ cap structure, a 7-methylguanosine (m7G) residue linked to the first nucleotide of the mRNA transcript, by proteins known as cap-binding proteins. This interaction plays a crucial role in various cellular processes, including:

1. **mRNA stability:** The 5' cap protects mRNA from degradation by exonucleases, extending its lifespan and allowing for efficient translation.
2. **mRNA translation initiation:** Cap-binding proteins, such as eukaryotic initiation factor 4E (eIF4E), recruit the mRNA to the ribosome, facilitating the initiation of protein synthesis.
3. **Nuclear export:** The cap structure is essential for mRNA export from the nucleus to the cytoplasm, where translation occurs.
4. **Splicing and polyadenylation:** The cap can also influence splicing and polyadenylation, processes that ensure the proper maturation of mRNA.

The interaction between cap-binding proteins and the 5' cap is highly specific and relies on a combination of electrostatic, hydrophobic, and hydrogen bonding interactions. This precise recognition ensures that only mature and functional mRNAs are processed and translated, contributing to the fidelity of gene expression.'
"

Proteins (2)

ProteinDefinitionTaxonomy
Eukaryotic initiation factor 4A-IA eukaryotic initiation factor 4A-I that is encoded in the genome of human. [PRO:DNx]Homo sapiens (human)
Eukaryotic translation initiation factor 4EA eukaryotic translation initiation factor 4E that is encoded in the genome of human. [PRO:DNx, UniProtKB:P06730]Homo sapiens (human)

Compounds (9)

CompoundDefinitionClassesRoles
cycloheximidecycloheximide : A dicarboximide that is 4-(2-hydroxyethyl)piperidine-2,6-dione in which one of the hydrogens attached to the carbon bearing the hydroxy group is replaced by a 3,5-dimethyl-2-oxocyclohexyl group. It is an antibiotic produced by the bacterium Streptomyces griseus.

Cycloheximide: Antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. It acts by inhibiting elongation during protein synthesis.
antibiotic fungicide;
cyclic ketone;
dicarboximide;
piperidine antibiotic;
piperidones;
secondary alcohol
anticoronaviral agent;
bacterial metabolite;
ferroptosis inhibitor;
neuroprotective agent;
protein synthesis inhibitor
rocaglamiderocaglamide : An organic heterotricyclic compound that is 2,3,3a,8b-tetrahydro-1H-benzo[b]cyclopenta[d]furan substituted by hydroxy groups at positions 1 and 8b, methoxy groups at positions 6 and 8, a 4-methoxyphenyl group at position 3a, a phenyl group at position 3 and a N,N-dimethylcarbamoyl group at position 1. Isolated from Aglaia odorata and Aglaia duperreana, it exhibits antineoplastic activity.

rocaglamide: RN refers to (1alpha,2alpha,3beta,3abeta,8bbeta)-isomer; isolated from stems of Aglaia elliptifolia; structure given in first source
monocarboxylic acid amide;
monomethoxybenzene;
organic heterotricyclic compound
antileishmanial agent;
antineoplastic agent;
metabolite
aglafolineaglafolin : A heterotricyclic compound based on a 2,3,3a,8b-tetrahydro-1H-benzo[b]cyclopenta[d]furan framework substituted by hydroxy groups at positions C-1 and C-8b, a methoxycarbonyl group at C-2, a phenyl group at C-3, a 4-methoxyphenyl group at C-3a and methoxy groups at C-6 and C-8. A platelet aggregation inhibitor found in Aglaia elliptifolia and Aglaia odorata.

aglafoline: a platelet activating factor antagonist; structure given in first source; isolated from Aglaia elliptifolia
methyl ester;
organic heterotricyclic compound
metabolite;
platelet aggregation inhibitor
sirolimussirolimus : A macrolide lactam isolated from Streptomyces hygroscopicus consisting of a 29-membered ring containing 4 trans double bonds, three of which are conjugated. It is an antibiotic, immunosupressive and antineoplastic agent.

Sirolimus: A macrolide compound obtained from Streptomyces hygroscopicus that acts by selectively blocking the transcriptional activation of cytokines thereby inhibiting cytokine production. It is bioactive only when bound to IMMUNOPHILINS. Sirolimus is a potent immunosuppressant and possesses both antifungal and antineoplastic properties.
antibiotic antifungal drug;
cyclic acetal;
cyclic ketone;
ether;
macrolide lactam;
organic heterotricyclic compound;
secondary alcohol
antibacterial drug;
anticoronaviral agent;
antineoplastic agent;
bacterial metabolite;
geroprotector;
immunosuppressive agent;
mTOR inhibitor
6-mercapto-7-methylguanosine6-mercapto-7-methylguanosine: structure given in first source
hippuristanolhippuristanol: from the gorgonian Isis hippuris
cgp 57380CGP 57380: inhibits the mitogen-activated protein kinase-interacting kinase Mnk1pyrazolopyrimidine
silvestrolsilvestrol : An organic heterotricyclic compound that consists of a 2,3,3a,8b-tetrahydro-H-benzo[b]cyclopenta[d]furan framework substituted by hydroxy groups at positions C-1 and C-8b, a methoxycarbonyl group at C-2, a phenyl group at C-3, a 4-methoxyphenyl group at C-3a, a methoxy group at C-8 and a 1,4-dioxan-2-yloxy group at position C-6 which in turn is substituted by a methoxy group at position 3 and a 1,2-dihydroxyethyl group at position 6. Isolated from Aglaia silvestris, it exhibits antineoplastic activity.

silvestrol: isolated from the fruit and twig of Aglaia silvestris
dioxanes;
ether;
methyl ester;
organic heterotricyclic compound
antineoplastic agent;
metabolite
7-methyl-GTPguanosine 5'-phosphate