Target type: biologicalprocess
The disaggregation of a protein-DNA complex into its constituent components. [GOC:mah]
Protein-DNA complex disassembly is a fundamental process in cellular biology, essential for regulating gene expression and various cellular processes. It involves the separation of a protein from a DNA molecule, breaking the interaction between them. This process can occur through several mechanisms, each tailored to the specific protein-DNA complex and the cellular context:
**1. Active Disassembly by Dedicated Enzymes:**
* **ATP-dependent Chromatin Remodeling Complexes:** These multi-protein complexes use the energy from ATP hydrolysis to reposition nucleosomes, the basic building blocks of chromatin, along the DNA strand. By disrupting the specific arrangement of nucleosomes, these complexes can facilitate access to DNA by other proteins, including transcription factors.
* **Helicase Enzymes:** These enzymes unwind DNA by breaking the hydrogen bonds between base pairs, leading to the separation of the DNA strands. Helicases are often involved in DNA replication and repair but can also contribute to protein-DNA complex disassembly by disrupting the protein-DNA interaction.
* **Topoisomerases:** These enzymes alter the topological state of DNA, relieving supercoiling and allowing DNA to unwind. They can contribute to protein-DNA complex disassembly by facilitating the movement of proteins along DNA.
**2. Passive Disassembly through Competition:**
* **Competitive Binding:** Other proteins can compete with the bound protein for DNA binding sites. This competition can be driven by differences in binding affinity, concentration, or specific DNA sequences targeted.
* **Diffusion:** Protein-DNA complexes are not static. The bound protein can diffuse along the DNA molecule, and if the binding site is not strong enough, the protein may eventually dissociate.
**3. Disassembly Triggered by Post-Translational Modifications:**
* **Phosphorylation:** Phosphorylation of specific amino acid residues within a protein can alter its conformation and weaken its binding affinity to DNA.
* **Ubiquitylation:** Addition of ubiquitin tags to proteins can target them for degradation by proteasomes, leading to the removal of the protein from the DNA complex.
* **Acetylation:** Acetylation of histones, the proteins that package DNA into chromatin, can modify the structure of chromatin, making it more accessible to other proteins and potentially disrupting existing protein-DNA interactions.
**The choice of disassembly mechanism is influenced by factors such as:**
* **The type of protein involved:** Different proteins have different binding affinities and mechanisms of DNA interaction.
* **The cellular context:** The presence of other proteins, the availability of ATP, and the overall cellular environment can all affect disassembly.
**The disruption of protein-DNA complex assembly is crucial for maintaining cellular function and regulating processes like:**
* **Gene transcription:** Regulating the access of transcription factors to DNA is essential for controlling gene expression.
* **DNA replication:** The unwinding of DNA during replication relies on the disassembly of existing protein-DNA complexes.
* **DNA repair:** DNA repair enzymes need to access damaged DNA, which may require the disassembly of protein-DNA complexes.
* **Chromatin remodeling:** The dynamic reorganization of chromatin is essential for various cellular processes, including gene expression and DNA replication.'
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Protein | Definition | Taxonomy |
---|---|---|
Myc proto-oncogene protein | A c-myc protein that is encoded in the genome of chicken. [OMA:P01109, PRO:DNx] | Gallus gallus (chicken) |
60S ribosomal protein L23 | A eukaryotic-type large ribosomal subunit protein uL14 that is encoded in the genome of human. [PRO:DNx, UniProtKB:P62829] | Homo sapiens (human) |
Myc proto-oncogene protein | A c-myc protein that is encoded in the genome of human. [PRO:CNA, UniProtKB:P01106] | Homo sapiens (human) |
Compound | Definition | Classes | Roles |
---|---|---|---|
aurintricarboxylic acid | aurintricarboxylic acid : A member of the class of quinomethanes that is 3-methylidene-6-oxocyclohexa-1,4-diene-1-carboxylic acid in which the methylidene hydrogens are replaced by 4-carboxy-3-hydroxyphenyl groups. The trisodium salt is the biological stain 'chrome violet CG' while the triammonium salt is 'aluminon'. Aurintricarboxylic Acid: A dye which inhibits protein biosynthesis at the initial stages. The ammonium salt (aluminon) is a reagent for the colorimetric estimation of aluminum in water, foods, and tissues. | monohydroxybenzoic acid; quinomethanes; tricarboxylic acid | fluorochrome; histological dye; insulin-like growth factor receptor 1 antagonist |
mefenamic acid | mefenamic acid : An aminobenzoic acid that is anthranilic acid in which one of the hydrogens attached to the nitrogen is replaced by a 2,3-dimethylphenyl group. Although classed as a non-steroidal anti-inflammatory drug, its anti-inflammatory properties are considered to be minor. It is used to relieve mild to moderate pain, including headaches, dental pain, osteoarthritis and rheumatoid arthritis. Mefenamic Acid: A non-steroidal anti-inflammatory agent with analgesic, anti-inflammatory, and antipyretic properties. It is an inhibitor of cyclooxygenase. | aminobenzoic acid; secondary amino compound | analgesic; antipyretic; antirheumatic drug; EC 1.14.99.1 (prostaglandin-endoperoxide synthase) inhibitor; environmental contaminant; non-steroidal anti-inflammatory drug; xenobiotic |
methyl red | methyl red : An azo dye consisting of benzoic acid substituted at position 2 by a 4-[(dimethylamino)phenyl]diazenyl group. methyl red: RN given refers to parent cpd; structure | ||
3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine | 1,2,4-triazines | ||
avasimibe | monoterpenoid | ||
resveratrol | trans-resveratrol : A resveratrol in which the double bond has E configuration. | resveratrol | antioxidant; phytoalexin; plant metabolite; quorum sensing inhibitor; radical scavenger |
10074-g5 | 10074-G5: structure in first source | ||
tws 119 | pyrroles | ||
gentamicin sulfate | |||
sgi-1027 | SGI-1027: inhibits DNA methyltransferase 1; structure in first source | ||
kj-pyr-9 | KJ-Pyr-9: antineoplastic; structure in first source | ||
PF-06446846 | PF-06446846 : A triazolopyridine that is 3H-[1,2,3]triazolo[4,5-b]pyridine substituted by a 4-{(3-chloropyridin-2-yl)[(3R)-piperidin-3-yl]carbamoyl}phenyl group at position 3. It is a potent inhibitor of PCSK9. PF-06446846: inhibits translation of PCSK9 ;structure in first source | benzamides; monochloropyridine; piperidines; tertiary carboxamide; triazolopyridine | antilipemic drug; EC 3.4.21.61 (kexin) inhibitor |