DNA recombinase assembly

Definition

Target type: biologicalprocess

The aggregation, arrangement and bonding together of strand exchange proteins (recombinases) into higher order oligomers on single-stranded DNA. [PMID:10357855]

DNA recombinases are enzymes that catalyze the exchange of genetic material between DNA molecules. This process is essential for many biological functions, including DNA repair, gene regulation, and the generation of genetic diversity. The assembly of a functional recombinase typically involves several steps:

1. **Binding to DNA:** The recombinase enzyme first binds to specific DNA sequences called recombination sites. These sites are often short, palindromic sequences that are recognized by the recombinase.
2. **Synapsis:** Once the recombinase has bound to the DNA, it brings together two DNA molecules that are to be recombined. This process is called synapsis.
3. **Strand exchange:** The recombinase then catalyzes the exchange of strands between the two DNA molecules. This exchange occurs at the recombination sites and results in the formation of a Holliday junction, which is a four-stranded DNA structure.
4. **Branch migration:** The Holliday junction can then migrate along the DNA molecule, exchanging more DNA strands. This process is called branch migration.
5. **Resolution:** Finally, the Holliday junction is resolved, resulting in the formation of two new DNA molecules that contain recombinant DNA.

The exact mechanism of DNA recombinase assembly can vary depending on the specific recombinase enzyme involved. However, the general principles outlined above are common to all recombinase enzymes.'
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Proteins (2)

Compounds (21)