Glyceraldehyde-3-phosphate dehydrogenase

A glyceraldehyde-3-phosphate dehydrogenase that is encoded in the genome of human. [PRO:DNx, UniProtKB:P04406]

Synonyms

GAPDH;
EC 1.2.1.12;
Peptidyl-cysteine S-nitrosylase GAPDH;
2.6.99.-

Research

Bioassay Publications (9)

Timeframe	Studies on this Protein(%)	All Drugs %
pre-1990	0 (0.00)	18.7374
1990's	4 (44.44)	18.2507
2000's	5 (55.56)	29.6817
2010's	0 (0.00)	24.3611
2020's	0 (0.00)	2.80

Compounds (18)

Drugs with Inhibition Measurements

Drug	Taxonomy	Measurement	Average (mM)	Bioassay(s)	Publication(s)
coumarin	Homo sapiens (human)	IC50	691.8310	1	1
oxidopamine	Homo sapiens (human)	IC50	8.0000	1	1
pentamidine	Homo sapiens (human)	IC50	50.0000	1	1
osthol	Homo sapiens (human)	IC50	208.9300	1	1
nicotinamide mononucleotide	Homo sapiens (human)	IC50	500.0000	2	2
2-methyladenine	Homo sapiens (human)	IC50	8,000.0000	3	3
isopentenyladenosine	Homo sapiens (human)	IC50	5,000.0000	1	1
benzonidazole	Homo sapiens (human)	IC50	50.0000	1	1
adenosine	Homo sapiens (human)	IC50	33,597.8571	7	7
adenosine	Homo sapiens (human)	Ki	2,500.0000	1	1
xanthyletine	Homo sapiens (human)	IC50	194.9840	1	1
metrifudil	Homo sapiens (human)	IC50	700.0000	1	1
seselin	Homo sapiens (human)	IC50	123.0270	1	1
n(6)-benzyladenosine	Homo sapiens (human)	IC50	4,200.0000	1	1
n(6)-benzyladenosine	Homo sapiens (human)	Ki	2,800.0000	1	1
2-methyladenosine	Homo sapiens (human)	IC50	8.0000	3	3
n(6)-cyclopentyladenosine	Homo sapiens (human)	IC50	5,000.0000	1	1
scopoletin	Homo sapiens (human)	IC50	257.0400	1	1
7-hydroxycoumarin	Homo sapiens (human)	IC50	616.5950	1	1

Drugs with Activation Measurements

Drug	Taxonomy	Measurement	Average (mM)	Bioassay(s)	Publication(s)
l 783281	Homo sapiens (human)	Kd	7.2000	1	1

Enables

This protein enables 9 target(s):

Target	Category	Definition
glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity	molecular function	Catalysis of the reaction: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH + H+. [EC:1.2.1.12]
protein binding	molecular function	Binding to a protein. [GOC:go_curators]
microtubule binding	molecular function	Binding to a microtubule, a filament composed of tubulin monomers. [GOC:krc]
aspartic-type endopeptidase inhibitor activity	molecular function	Binds to and stops, prevents or reduces the activity of aspartic-type endopeptidases. [GOC:ai]
peptidyl-cysteine S-nitrosylase activity	molecular function	Catalysis of the transfer of a nitric oxide (NO) group to a sulphur atom within a cysteine residue of a protein. [GOC:sp, PMID:20972425, PMID:20972426]
identical protein binding	molecular function	Binding to an identical protein or proteins. [GOC:jl]
NADP binding	molecular function	Binding to nicotinamide-adenine dinucleotide phosphate, a coenzyme involved in many redox and biosynthetic reactions; binding may be to either the oxidized form, NADP+, or the reduced form, NADPH. [GOC:ai]
NAD binding	molecular function	Binding to nicotinamide adenine dinucleotide, a coenzyme involved in many redox and biosynthetic reactions; binding may be to either the oxidized form, NAD+, or the reduced form, NADH. [GOC:ai]
disordered domain specific binding	molecular function	Binding to a disordered domain of a protein. [GOC:gg, PMID:11746698]

Located In

This protein is located in 12 target(s):

Target	Category	Definition
nucleus	cellular component	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. [GOC:go_curators]
cytoplasm	cellular component	The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. [ISBN:0198547684]
lipid droplet	cellular component	An intracellular non-membrane-bounded organelle comprising a matrix of coalesced lipids surrounded by a phospholipid monolayer. May include associated proteins. [GOC:mah, GOC:tb]
cytosol	cellular component	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. [GOC:hjd, GOC:jl]
plasma membrane	cellular component	The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. [ISBN:0716731363]
microtubule cytoskeleton	cellular component	The part of the cytoskeleton (the internal framework of a cell) composed of microtubules and associated proteins. [GOC:jl, ISBN:0395825172]
membrane	cellular component	A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. [GOC:dos, GOC:mah, ISBN:0815316194]
nuclear membrane	cellular component	Either of the lipid bilayers that surround the nucleus and form the nuclear envelope; excludes the intermembrane space. [GOC:mah, GOC:pz]
vesicle	cellular component	Any small, fluid-filled, spherical organelle enclosed by membrane. [GOC:mah, GOC:pz, GOC:vesicles]
intracellular membrane-bounded organelle	cellular component	Organized structure of distinctive morphology and function, bounded by a single or double lipid bilayer membrane and occurring within the cell. Includes the nucleus, mitochondria, plastids, vacuoles, and vesicles. Excludes the plasma membrane. [GOC:go_curators]
perinuclear region of cytoplasm	cellular component	Cytoplasm situated near, or occurring around, the nucleus. [GOC:jid]
extracellular exosome	cellular component	A vesicle that is released into the extracellular region by fusion of the limiting endosomal membrane of a multivesicular body with the plasma membrane. Extracellular exosomes, also simply called exosomes, have a diameter of about 40-100 nm. [GOC:BHF, GOC:mah, GOC:vesicles, PMID:15908444, PMID:17641064, PMID:19442504, PMID:19498381, PMID:22418571, PMID:24009894]

Active In

This protein is active in 1 target(s):

Target	Category	Definition
cytosol	cellular component	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. [GOC:hjd, GOC:jl]

Part Of

This protein is part of 2 target(s):

Target	Category	Definition
GAIT complex	cellular component	A protein complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. The complex binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs and suppresses their translation by blocking the recruitment of the 43S ribosomal complex to m7G cap-bound eIF4G. In humans it includes RPL13A, EPRS, SYNCRIP and GAPDH; mouse complexes lack SYNCRIP. [GOC:br, PMID:15479637, PMID:23071094]
ribonucleoprotein complex	cellular component	A macromolecular complex that contains both RNA and protein molecules. [GOC:krc, GOC:vesicles]

Involved In

This protein is involved in 17 target(s):

Target	Category	Definition
microtubule cytoskeleton organization	biological process	A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising microtubules and their associated proteins. [GOC:mah]
positive regulation of cytokine production	biological process	Any process that activates or increases the frequency, rate or extent of production of a cytokine. [GOC:add, ISBN:0781735149]
glucose metabolic process	biological process	The chemical reactions and pathways involving glucose, the aldohexose gluco-hexose. D-glucose is dextrorotatory and is sometimes known as dextrose; it is an important source of energy for living organisms and is found free as well as combined in homo- and hetero-oligosaccharides and polysaccharides. [ISBN:0198506732]
glycolytic process	biological process	The chemical reactions and pathways resulting in the breakdown of a carbohydrate into pyruvate, with the concomitant production of a small amount of ATP and the reduction of NAD(P) to NAD(P)H. Glycolysis begins with the metabolism of a carbohydrate to generate products that can enter the pathway and ends with the production of pyruvate. Pyruvate may be converted to acetyl-coenzyme A, ethanol, lactate, or other small molecules. [GOC:bf, GOC:dph, ISBN:0201090910, ISBN:0716720094, ISBN:0879010479, Wikipedia:Glycolysis]
negative regulation of endopeptidase activity	biological process	Any process that decreases the frequency, rate or extent of endopeptidase activity, the endohydrolysis of peptide bonds within proteins. [GOC:dph, GOC:tb]
regulation of macroautophagy	biological process	Any process that modulates the frequency, rate or extent of macroautophagy. [GOC:krc]
negative regulation of translation	biological process	Any process that stops, prevents, or reduces the frequency, rate or extent of the chemical reactions and pathways resulting in the formation of proteins by the translation of mRNA or circRNA. [GOC:isa_complete]
killing of cells of another organism	biological process	Any process in an organism that results in the killing of cells of another organism, including in some cases the death of the other organism. Killing here refers to the induction of death in one cell by another cell, not cell-autonomous death due to internal or other environmental conditions. [GOC:add]
positive regulation of type I interferon production	biological process	Any process that activates or increases the frequency, rate, or extent of type I interferon production. Type I interferons include the interferon-alpha, beta, delta, episilon, zeta, kappa, tau, and omega gene families. [GOC:add, GOC:mah]
peptidyl-cysteine S-trans-nitrosylation	biological process	Transfer of a nitric oxide (NO) group from one cysteine residue to another. [PMID:19854201, PMID:20972425, PMID:20972426]
positive regulation of canonical NF-kappaB signal transduction	biological process	Any process that activates or increases the frequency, rate or extent of a canonical NF-kappaB signaling cascade. [GOC:jl]
protein stabilization	biological process	Any process involved in maintaining the structure and integrity of a protein and preventing it from degradation or aggregation. [GOC:ai]
defense response to fungus	biological process	Reactions triggered in response to the presence of a fungus that act to protect the cell or organism. [GOC:ai]
neuron apoptotic process	biological process	Any apoptotic process in a neuron, the basic cellular unit of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the nervous system. [CL:0000540, GOC:mtg_apoptosis]
killing by host of symbiont cells	biological process	Any process mediated by an organism that results in the death of cells in the symbiont organism. The symbiont is defined as the smaller of the organisms involved in a symbiotic interaction. [GOC:add]
antimicrobial humoral immune response mediated by antimicrobial peptide	biological process	An immune response against microbes mediated by anti-microbial peptides in body fluid. [PMID:15761415, PMID:24287494]
cellular response to type II interferon	biological process	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an interferon-gamma stimulus. Interferon gamma is the only member of the type II interferon found so far. [GOC:mah]