acetylglucosaminyltransferase activity
Definition
Target type: molecularfunction
Catalysis of the transfer of an N-acetylglucosaminyl residue from UDP-N-acetyl-glucosamine to a sugar. [ISBN:0198506732]
Acetylglucosaminyltransferase activity refers to the enzymatic transfer of N-acetylglucosamine (GlcNAc) from a donor molecule, typically UDP-GlcNAc, to an acceptor molecule. This enzymatic reaction is crucial for various biological processes, including protein glycosylation, synthesis of glycosaminoglycans, and the assembly of glycoconjugates.
The molecular mechanism of acetylglucosaminyltransferase activity involves the following steps:
1. **Substrate Binding:** The enzyme binds to both the donor molecule (UDP-GlcNAc) and the acceptor molecule. The acceptor molecule can be a protein, lipid, or another carbohydrate.
2. **Nucleotide Diphosphate Release:** The enzyme catalyzes the release of UDP from the UDP-GlcNAc, leaving the GlcNAc molecule attached to the enzyme.
3. **GlcNAc Transfer:** The enzyme transfers the GlcNAc molecule to the acceptor molecule, forming a glycosidic bond.
4. **Product Release:** The enzyme releases the glycosylated product and is ready to repeat the cycle.
The specificity of acetylglucosaminyltransferases is determined by the specific amino acid residues within the enzyme's active site, which interact with both the donor and acceptor molecules. This specificity ensures that the GlcNAc is transferred to the correct acceptor molecule at the appropriate location.
The resulting glycosylated product can have various biological functions, including:
* **Protein folding and stability:** Glycosylation can influence protein folding and stability, affecting its function and half-life.
* **Cell signaling:** Glycosylated proteins can act as receptors, ligands, or adhesion molecules, mediating cell-cell interactions and signaling pathways.
* **Immune recognition:** Glycosylation patterns on cell surfaces can serve as markers for immune recognition, influencing immune responses and immune evasion.
* **Protease resistance:** Glycosylation can protect proteins from degradation by proteases, extending their lifespan and function.
Variations in acetylglucosaminyltransferase activity can lead to various diseases, including cancer, genetic disorders, and immune deficiencies. Therefore, understanding the molecular function of acetylglucosaminyltransferase activity is essential for developing therapeutic interventions for these conditions.'
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Proteins (3)
| Protein | Definition | Taxonomy |
|---|---|---|
| Beta-hexosaminidase subunit beta | A beta-hexosaminidase subunit beta that is encoded in the genome of human. [PRO:DNx, UniProtKB:P07686] | Homo sapiens (human) |
| Beta-hexosaminidase subunit alpha | A beta-hexosaminidase subunit alpha that is encoded in the genome of human. [PRO:DNx, UniProtKB:P06865] | Homo sapiens (human) |
| UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit | A UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit that is encoded in the genome of human. [PRO:DNx, UniProtKB:O15294] | Homo sapiens (human) |
Compounds (12)
| Compound | Definition | Classes | Roles |
|---|---|---|---|
| pyrimethamine | Maloprim: contains above 2 cpds | aminopyrimidine; monochlorobenzenes | antimalarial; antiprotozoal drug; EC 1.5.1.3 (dihydrofolate reductase) inhibitor |
| uridine diphosphate | Uridine Diphosphate: A uracil nucleotide containing a pyrophosphate group esterified to C5 of the sugar moiety. | pyrimidine ribonucleoside 5'-diphosphate; uridine 5'-phosphate | Escherichia coli metabolite; mouse metabolite |
| benzoxazolone | 2-benzoxazolinone : A member of the class of benzoxazoles that is 2,3-dihydro-1,3-benzoxazole carrying an oxo group at position 2. benzoxazolone: RN given refers to parent cpd; structure | benzoxazole | allelochemical; phytoalexin |
| 4-ethynylbiphenyl | 4-ethynylbiphenyl: structure given in first source | ||
| naphthalimides | Naphthalimides: Compounds with three fused rings that appear like a naphthalene fused to piperidone or like a benz(de)isoquinoline-1,3-dione (not to be confused with BENZYLISOQUINOLINES which have a methyl separating the naphthyl from the benzyl rings). Members are CYTOTOXINS. | ||
| 2-ethynylnaphthalene | 2-ethynylnaphthalene: RN given refers to unlabeled parent cpd | ||
| 2-acetamido-1,5-imino-1,2,5-trideoxy-d-glucitol | 2-acetamido-1,5-imino-1,2,5-trideoxy-D-glucitol: structure given in first source | ||
| 2-(2-oxolanylmethyl)benzo[de]isoquinoline-1,3-dione | isoquinolines | ||
| n-acetylglucosamine thiazoline | N-acetylglucosamine thiazoline: an analog of the oxazolinium bicyclic intermediate leading from N-acetylglucosamine to 1,6-anhydro-N-acetylmuramic acid | ||
| urolithin d | urolithin D: has antiproliferative activity; structure in first source | hydroxycoumarin | |
| tetrahydroamentoflavone | tetrahydroamentoflavone: isolated from Semecarpus anacardium; structure in first source | ||
| thiamet g |