glycylpeptide N-tetradecanoyltransferase activity

Definition

Target type: molecularfunction

Catalysis of the reaction: tetradecanoyl-CoA + glycyl-peptide = CoA + N-tetradecanoylglycyl-peptide. [EC:2.3.1.97]

Glycylpeptide N-tetradecanoyltransferase activity is a crucial enzyme activity involved in the biosynthesis of mycobacterial cell wall components. It catalyzes the transfer of a myristate group from myristoyl-CoA to the N-terminal glycine residue of a specific peptide substrate. This reaction plays a significant role in the formation of the mycolyl-arabinogalactan layer, a key component of the mycobacterial cell wall responsible for its structural integrity and resistance to antibiotics. The enzyme's activity involves several steps: 1. Binding of both myristoyl-CoA and the peptide substrate to the enzyme's active site. 2. Formation of a covalent intermediate between the enzyme and myristoyl-CoA. 3. Transfer of the myristate group from the enzyme-myristoyl-CoA intermediate to the N-terminal glycine residue of the peptide substrate. 4. Release of the modified peptide and CoA from the enzyme. This enzymatic activity is essential for the survival and pathogenesis of Mycobacterium tuberculosis, the causative agent of tuberculosis. Inhibiting this activity could potentially lead to the development of new anti-tuberculosis drugs.'
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Proteins (4)

Compounds (3)