[protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine/L-threonine O-N-acetyl-alpha-D-glucosaminase activity

Definition

Target type: molecularfunction

Catalysis of the reaction: 3-O-(N-acetyl-beta-D-glucosaminyl)-L-seryl/L-threonyl-[protein] + H2O = L-seryl//L-threonyl-[protein] + N-acetyl-D-glucosamine. [EC:3.2.1.169]

This enzyme catalyzes the hydrolysis of the N-acetyl-D-glucosamine moiety from the O-linked N-acetyl-D-glucosamine (GlcNAc) modification on serine or threonine residues of proteins. This modification, known as O-GlcNAcylation, is a dynamic and reversible post-translational modification that plays crucial roles in various cellular processes, including transcription, signal transduction, and protein trafficking. The enzyme specifically removes the terminal GlcNAc residue from the O-GlcNAc-modified protein, thereby de-glycosylating the substrate and potentially altering its function. It is involved in the regulation of the cellular O-GlcNAc cycle, which is a balance between O-GlcNAc transferase (OGT) activity, responsible for adding GlcNAc to proteins, and O-GlcNAcase (OGA) activity, responsible for removing it. OGA plays a crucial role in maintaining cellular homeostasis by regulating protein function and signaling pathways through its O-GlcNAc de-glycosylation activity.'
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Proteins (1)

Compounds (4)