Target type: molecularfunction
Catalysis of the reaction: DNA containing 7-methyladenine + H2O = DNA with abasic site + 7-methyladenine. This reaction is the hydrolysis of DNA by cleavage of the N-C1' glycosidic bond between the damaged DNA 7-methyladenine and the deoxyribose sugar to remove the 7-methyladenine, leaving an abasic site. [GOC:jl, PMID:16468998]
DNA-7-methyladenine glycosylase activity refers to the enzymatic removal of 7-methyl adenine (7mA) from DNA. 7mA is a minor base modification found in prokaryotes and eukaryotes, where it can be involved in various biological processes, including DNA repair, gene regulation, and viral defense. This activity is crucial for maintaining genome integrity and preventing mutations.
The process starts with the recognition of 7mA by the glycosylase enzyme. The enzyme specifically binds to the modified base, flipping it out of the DNA helix. Once bound, the glycosylase cleaves the N-glycosidic bond between 7mA and the deoxyribose sugar backbone, creating an apurinic/apyrimidinic (AP) site.
The AP site is then processed by other DNA repair enzymes, such as AP endonucleases, which cleave the phosphodiester backbone adjacent to the AP site. This creates a gap in the DNA strand, which can be filled in by DNA polymerase using the undamaged strand as a template.
The removal of 7mA by DNA-7-methyladenine glycosylase activity ensures that the DNA sequence is accurately maintained and prevents the accumulation of potentially harmful mutations. This activity is vital for cellular function and survival.'
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| Protein | Definition | Taxonomy |
|---|---|---|
| DNA-3-methyladenine glycosylase | A DNA-3-methyladenine glycosylase that is encoded in the genome of human. [PRO:DNx, UniProtKB:P29372] | Homo sapiens (human) |
| Compound | Definition | Classes | Roles |
|---|---|---|---|
| aurintricarboxylic acid | aurintricarboxylic acid : A member of the class of quinomethanes that is 3-methylidene-6-oxocyclohexa-1,4-diene-1-carboxylic acid in which the methylidene hydrogens are replaced by 4-carboxy-3-hydroxyphenyl groups. The trisodium salt is the biological stain 'chrome violet CG' while the triammonium salt is 'aluminon'. Aurintricarboxylic Acid: A dye which inhibits protein biosynthesis at the initial stages. The ammonium salt (aluminon) is a reagent for the colorimetric estimation of aluminum in water, foods, and tissues. | monohydroxybenzoic acid; quinomethanes; tricarboxylic acid | fluorochrome; histological dye; insulin-like growth factor receptor 1 antagonist |
| gossypol | Gossypol: A dimeric sesquiterpene found in cottonseed (GOSSYPIUM). The (-) isomer is active as a male contraceptive (CONTRACEPTIVE AGENTS, MALE) whereas toxic symptoms are associated with the (+) isomer. | ||
| mitoxantrone hydrochloride | hydrochloride | antineoplastic agent | |
| morin | morin : A pentahydroxyflavone that is 7-hydroxyflavonol bearing three additional hydroxy substituents at positions 2' 4' and 5. morin: a light yellowish pigment found in the wood of old fustic (Chlorophora tinctoria) | 7-hydroxyflavonol; pentahydroxyflavone | angiogenesis modulating agent; anti-inflammatory agent; antibacterial agent; antihypertensive agent; antineoplastic agent; antioxidant; EC 5.99.1.2 (DNA topoisomerase) inhibitor; hepatoprotective agent; metabolite; neuroprotective agent |