tyrosyl-RNA phosphodiesterase activity
Definition
Target type: molecularfunction
Catalysis of the hydrolysis of a 5' tyrosyl-RNA phosphodiester bond between a protein and RNA. In picornaviruses, this covalent bond connects VPg, a viral-encoded protein essential for RNA replication, to the 5' end of all nascent picornavirus genomes; it is cleaved from viral RNA prior to its engaging in protein synthesis. [GOC:bf, GOC:sp, PMID:21408223, PMID:22908287]
Tyrosyl-RNA phosphodiesterase activity involves the hydrolysis of a phosphodiester bond within a tyrosyl-RNA molecule. This activity is crucial for the process of tRNA splicing, a critical step in the maturation of transfer RNA molecules. In tRNA splicing, the introns are removed from the precursor tRNA molecule, and the exons are ligated together. This process is facilitated by a complex of enzymes, including a tyrosyl-RNA phosphodiesterase.
The enzyme cleaves the phosphodiester bond between the 5' end of the intron and the 3' end of the exon, generating a tyrosyl-RNA intermediate. This intermediate is then subjected to further processing, leading to the removal of the intron and the ligation of the exons.
Tyrosyl-RNA phosphodiesterase activity is essential for the proper production of functional tRNA molecules. Without this activity, tRNA splicing would be incomplete, and the resulting tRNA molecules would be unable to participate in protein synthesis. This could lead to a wide range of cellular dysfunctions and potentially severe consequences for the organism.'
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Proteins (1)
| Protein | Definition | Taxonomy |
|---|---|---|
| Tyrosyl-DNA phosphodiesterase 2 | A tyrosyl-DNA phosphodiesterase 2 that is encoded in the genome of human. [PRO:DNx, UniProtKB:O95551] | Homo sapiens (human) |
Compounds (10)
| Compound | Definition | Classes | Roles |
|---|---|---|---|
| beta-lapachone | beta-lapachone : A benzochromenone that is 3,4-dihydro-2H-benzo[h]chromene-5,6-dione substituted by geminal methyl groups at position 2. Isolated from Tabebuia avellanedae, it exhibits antineoplastic and anti-inflammatory activities. beta-lapachone: antineoplastic inhibitor of reverse transcriptase, DNA topoisomerase, and DNA polymerase | benzochromenone; orthoquinones | anti-inflammatory agent; antineoplastic agent; plant metabolite |
| camptothecin | NSC 100880: carboxylate (opened lactone) form of camptothecin; RN refers to (S)-isomer; structure given in first source | delta-lactone; pyranoindolizinoquinoline; quinoline alkaloid; tertiary alcohol | antineoplastic agent; EC 5.99.1.2 (DNA topoisomerase) inhibitor; genotoxin; plant metabolite |
| n,n'-dibenzoylcystine | N,N'-dibenzoylcystine: a low molecular weight gelator; structure in first source | ||
| nsc 95397 | 1,4-naphthoquinones | ||
| 1,3(2h,4h)-isoquinolinedione | 1,3(2H,4H)-isoquinolinedione: structure in first source | ||
| nsc 706744 | |||
| 1,3,6-trimethylpyrimido[5,4-e][1,2,4]triazine-5,7-dione | pyrimidotriazine | ||
| N-(7-phenyl-[1,2,4]triazolo[1,5-a]pyrimidin-2-yl)benzamide | pyrimidines | ||
| 1,6-dimethyl-3-propylpyrimido[5,4-e][1,2,4]triazine-5,7-dione | pyrimidotriazine | ||
| 2,3,4,10-tetrahydro-7,10-dimethyl-2,4-dioxobenzo(g)pteridine | flavin |