Tyrosyl-DNA phosphodiesterase 2

A tyrosyl-DNA phosphodiesterase 2 that is encoded in the genome of human. [PRO:DNx, UniProtKB:O95551]

Synonyms

Tyr-DNA phosphodiesterase 2;
hTDP2;
EC 3.1.4.-;
5'-tyrosyl-DNA phosphodiesterase;
5'-Tyr-DNA phosphodiesterase;
ETS1-associated protein 2;
ETS1-associated protein II;
EAPII;
TRAF and TNF receptor-associated protein;
Ty

Research

Bioassay Publications (6)

Timeframe	Studies on this Protein(%)	All Drugs %
pre-1990	0 (0.00)	18.7374
1990's	0 (0.00)	18.2507
2000's	0 (0.00)	29.6817
2010's	6 (100.00)	24.3611
2020's	0 (0.00)	2.80

Compounds (10)

Drugs with Inhibition Measurements

Drug	Taxonomy	Measurement	Average (mM)	Bioassay(s)	Publication(s)
beta-lapachone	Homo sapiens (human)	IC50	0.9700	1	1
camptothecin	Homo sapiens (human)	IC50	111.0000	1	1
n,n'-dibenzoylcystine	Homo sapiens (human)	IC50	4.1000	1	1
nsc 95397	Homo sapiens (human)	IC50	71.0000	1	1
1,3(2h,4h)-isoquinolinedione	Homo sapiens (human)	IC50	25.0000	1	1
nsc 706744	Homo sapiens (human)	IC50	1.0000	1	1
N-(7-phenyl-[1,2,4]triazolo[1,5-a]pyrimidin-2-yl)benzamide	Homo sapiens (human)	IC50	100.0000	1	1
1,6-dimethyl-3-propylpyrimido[5,4-e][1,2,4]triazine-5,7-dione	Homo sapiens (human)	IC50	1.5100	1	1
2,3,4,10-tetrahydro-7,10-dimethyl-2,4-dioxobenzo(g)pteridine	Homo sapiens (human)	IC50	3.5000	1	1

Drugs with Activation Measurements

Drug	Taxonomy	Measurement	Average (mM)	Bioassay(s)	Publication(s)
1,3,6-trimethylpyrimido[5,4-e][1,2,4]triazine-5,7-dione	Homo sapiens (human)	EC50	1.1600	1	1
1,6-dimethyl-3-propylpyrimido[5,4-e][1,2,4]triazine-5,7-dione	Homo sapiens (human)	EC50	0.5100	1	1
2,3,4,10-tetrahydro-7,10-dimethyl-2,4-dioxobenzo(g)pteridine	Homo sapiens (human)	EC50	3.5000	1	1

Enables

This protein enables 8 target(s):

Target	Category	Definition
magnesium ion binding	molecular function	Binding to a magnesium (Mg) ion. [GOC:ai]
single-stranded DNA binding	molecular function	Binding to single-stranded DNA. [GOC:elh, GOC:vw, PMID:22976174]
transcription corepressor activity	molecular function	A transcription coregulator activity that represses or decreases the transcription of specific gene sets via binding to a DNA-bound DNA-binding transcription factor, either on its own or as part of a complex. Corepressors often act by altering chromatin structure and modifications. For example, one class of transcription corepressors modifies chromatin structure through covalent modification of histones. A second class remodels the conformation of chromatin in an ATP-dependent fashion. A third class modulates interactions of DNA-bound DNA-binding transcription factors with other transcription coregulators. [GOC:txnOH-2018, PMID:10213677, PMID:16858867]
nuclease activity	molecular function	Catalysis of the hydrolysis of ester linkages within nucleic acids. [ISBN:0198547684]
protein binding	molecular function	Binding to a protein. [GOC:go_curators]
manganese ion binding	molecular function	Binding to a manganese ion (Mn). [GOC:ai]
tyrosyl-RNA phosphodiesterase activity	molecular function	Catalysis of the hydrolysis of a 5' tyrosyl-RNA phosphodiester bond between a protein and RNA. In picornaviruses, this covalent bond connects VPg, a viral-encoded protein essential for RNA replication, to the 5' end of all nascent picornavirus genomes; it is cleaved from viral RNA prior to its engaging in protein synthesis. [GOC:bf, GOC:sp, PMID:21408223, PMID:22908287]
5'-tyrosyl-DNA phosphodiesterase activity	molecular function	Catalysis of the hydrolysis of 5'-phosphotyrosyl groups formed as covalent intermediates (in DNA backbone breakage) between DNA topoisomerase II and DNA. [PMID:16751265]

Located In

This protein is located in 7 target(s):

Target	Category	Definition
nucleus	cellular component	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. [GOC:go_curators]
nucleoplasm	cellular component	That part of the nuclear content other than the chromosomes or the nucleolus. [GOC:ma, ISBN:0124325653]
nucleolus	cellular component	A small, dense body one or more of which are present in the nucleus of eukaryotic cells. It is rich in RNA and protein, is not bounded by a limiting membrane, and is not seen during mitosis. Its prime function is the transcription of the nucleolar DNA into 45S ribosomal-precursor RNA, the processing of this RNA into 5.8S, 18S, and 28S components of ribosomal RNA, and the association of these components with 5S RNA and proteins synthesized outside the nucleolus. This association results in the formation of ribonucleoprotein precursors; these pass into the cytoplasm and mature into the 40S and 60S subunits of the ribosome. [ISBN:0198506732]
cytoplasm	cellular component	The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. [ISBN:0198547684]
aggresome	cellular component	An inclusion body formed by dynein-dependent retrograde transport of an aggregated protein on microtubules. [PMID:11121744]
nuclear body	cellular component	Extra-nucleolar nuclear domains usually visualized by confocal microscopy and fluorescent antibodies to specific proteins. [GOC:ma, PMID:10330182]
PML body	cellular component	A class of nuclear body; they react against SP100 auto-antibodies (PML, promyelocytic leukemia); cells typically contain 10-30 PML bodies per nucleus; alterations in the localization of PML bodies occurs after viral infection. [GOC:ma, PMID:10944585]

Active In

This protein is active in 2 target(s):

Target	Category	Definition
PML body	cellular component	A class of nuclear body; they react against SP100 auto-antibodies (PML, promyelocytic leukemia); cells typically contain 10-30 PML bodies per nucleus; alterations in the localization of PML bodies occurs after viral infection. [GOC:ma, PMID:10944585]
cytoplasm	cellular component	The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. [ISBN:0198547684]

Involved In

This protein is involved in 4 target(s):

Target	Category	Definition
double-strand break repair	biological process	The repair of double-strand breaks in DNA via homologous and nonhomologous mechanisms to reform a continuous DNA helix. [GOC:elh]
cell surface receptor signaling pathway	biological process	The series of molecular signals initiated by an extracellular ligand binding to a receptor located on the cell surface. The pathway ends with regulation of a downstream cellular process, e.g. transcription. [GOC:signaling]
negative regulation of DNA-templated transcription	biological process	Any process that stops, prevents, or reduces the frequency, rate or extent of cellular DNA-templated transcription. [GOC:go_curators, GOC:txnOH]
neuron development	biological process	The process whose specific outcome is the progression of a neuron over time, from initial commitment of the cell to a specific fate, to the fully functional differentiated cell. [GOC:dph]