DNA-3-methyladenine glycosylase activity
Definition
Target type: molecularfunction
Catalysis of the reaction: DNA containing 3-methyladenine + H2O = DNA with abasic site + 3-methyladenine. This reaction is the hydrolysis of DNA by cleavage of the N-C1' glycosidic bond between the damaged DNA 3-methyladenine and the deoxyribose sugar to remove the 3-methyladenine, leaving an abasic site. [EC:3.2.2.20, GOC:elh, PMID:10872450, PMID:9224623]
DNA-3-methyladenine glycosylase activity is a crucial molecular function involved in the repair of damaged DNA. It specifically targets and removes 3-methyladenine (3-meA) from DNA, a common DNA lesion caused by alkylating agents. This enzyme plays a critical role in maintaining genomic integrity by restoring the correct nucleotide sequence. The process begins with the enzyme recognizing and binding to 3-meA within the DNA strand. This recognition event triggers a series of conformational changes within the enzyme, leading to the cleavage of the glycosidic bond linking the 3-meA base to the deoxyribose sugar backbone. The resulting abasic site (a site lacking a base) is then processed by other DNA repair pathways, such as base excision repair (BER), to restore the original DNA sequence. This activity is essential for protecting the genome from the mutagenic effects of alkylating agents, which can cause cancer and other diseases. The removal of 3-meA by this enzyme ensures the fidelity of DNA replication and gene expression, preventing mutations and maintaining the stability of the genetic code.'
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Proteins (1)
| Protein | Definition | Taxonomy |
|---|---|---|
| DNA-3-methyladenine glycosylase | A DNA-3-methyladenine glycosylase that is encoded in the genome of human. [PRO:DNx, UniProtKB:P29372] | Homo sapiens (human) |
Compounds (4)
| Compound | Definition | Classes | Roles |
|---|---|---|---|
| aurintricarboxylic acid | aurintricarboxylic acid : A member of the class of quinomethanes that is 3-methylidene-6-oxocyclohexa-1,4-diene-1-carboxylic acid in which the methylidene hydrogens are replaced by 4-carboxy-3-hydroxyphenyl groups. The trisodium salt is the biological stain 'chrome violet CG' while the triammonium salt is 'aluminon'. Aurintricarboxylic Acid: A dye which inhibits protein biosynthesis at the initial stages. The ammonium salt (aluminon) is a reagent for the colorimetric estimation of aluminum in water, foods, and tissues. | monohydroxybenzoic acid; quinomethanes; tricarboxylic acid | fluorochrome; histological dye; insulin-like growth factor receptor 1 antagonist |
| gossypol | Gossypol: A dimeric sesquiterpene found in cottonseed (GOSSYPIUM). The (-) isomer is active as a male contraceptive (CONTRACEPTIVE AGENTS, MALE) whereas toxic symptoms are associated with the (+) isomer. | ||
| mitoxantrone hydrochloride | hydrochloride | antineoplastic agent | |
| morin | morin : A pentahydroxyflavone that is 7-hydroxyflavonol bearing three additional hydroxy substituents at positions 2' 4' and 5. morin: a light yellowish pigment found in the wood of old fustic (Chlorophora tinctoria) | 7-hydroxyflavonol; pentahydroxyflavone | angiogenesis modulating agent; anti-inflammatory agent; antibacterial agent; antihypertensive agent; antineoplastic agent; antioxidant; EC 5.99.1.2 (DNA topoisomerase) inhibitor; hepatoprotective agent; metabolite; neuroprotective agent |