MAML1-RBP-Jkappa- ICN1 complex

Definition

Target type: cellularcomponent

A protein complex that consists of the intracellular domain of Notch1 (ICN1), the DNA-binding transcription factor RBP-Jkappa, and the transcriptional coactivator Mastermind-like-1 (MAML1); the complex is involved in transcriptional activation in response to Notch-mediated signaling. [CORUM:2949, PMID:16510869]

The MAML1-RBP-Jkappa-ICN1 complex is a multi-protein complex that plays a critical role in Notch signaling. It is located in the nucleus and functions as a transcription factor, regulating the expression of genes involved in cell fate decisions, differentiation, and development. Here's a detailed description of the cellular component of this complex:

- **MAML1 (Mastermind-like 1):** This protein acts as a scaffold, bringing together the other components of the complex. MAML1 has a transactivation domain that interacts with other transcription factors to enhance gene expression.

- **RBP-Jkappa (Recombination signal binding protein-J kappa):** This protein is a DNA-binding factor that recognizes and binds to specific DNA sequences called CSL sites (CBF1/Su(H)/Lag-1). It acts as a transcriptional repressor in the absence of Notch signaling. Upon Notch activation, it undergoes a conformational change, allowing the recruitment of MAML1 and ICN1.

- **ICN1 (Intracellular domain of Notch1):** This protein is generated upon the cleavage of the Notch receptor by a proteolytic cascade. ICN1 translocates to the nucleus and interacts with the RBP-Jkappa-MAML1 complex. It contains a transactivation domain that activates gene expression.

- **The complex assembles on specific DNA sequences:** The MAML1-RBP-Jkappa-ICN1 complex assembles on DNA at CSL sites, which are located in the regulatory regions of target genes. This binding event is essential for the complex to exert its transcriptional activity.

- **The complex interacts with other transcription factors:** The complex can interact with other transcription factors, both activators and repressors, to regulate the expression of specific genes. This interaction network contributes to the fine-tuning of gene expression and the diverse biological functions of Notch signaling.

- **The complex undergoes dynamic changes:** The composition and activity of the MAML1-RBP-Jkappa-ICN1 complex can vary depending on the cellular context and signaling pathways involved. For example, different Notch receptors can interact with the complex, leading to the activation of distinct sets of target genes.

In summary, the MAML1-RBP-Jkappa-ICN1 complex is a dynamic and multifaceted nuclear complex that plays a central role in Notch signaling by modulating gene expression in a precise and context-dependent manner. It is essential for proper development, differentiation, and cell fate determination.'
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Proteins (1)

Compounds (1)