Page last updated: 2024-10-24

negative regulation of lyase activity

Definition

Target type: biologicalprocess

Any process that stops or reduces the rate of lyase activity, the catalysis of the cleavage of C-C, C-O, C-N and other bonds by other means than by hydrolysis or oxidation, or conversely adding a group to a double bond. [GOC:ai]

Negative regulation of lyase activity involves the control of enzymes known as lyases. Lyases are a class of enzymes that catalyze the breaking of chemical bonds, particularly C-C, C-O, C-N, and C-S bonds, through a process called lysis. This lysis is achieved without the addition of water, unlike hydrolases. Lyases are involved in a wide array of cellular processes, including metabolism, DNA repair, and biosynthesis.

The regulation of lyase activity is crucial for maintaining cellular homeostasis and ensuring proper functioning of biological pathways. Negative regulation of lyase activity implies the inhibition or suppression of lyase enzyme function. This inhibition can be achieved through various mechanisms:

1. **Allosteric Regulation:** In this mechanism, a regulatory molecule binds to a site on the lyase enzyme that is distinct from the active site. This binding alters the enzyme's conformation, thereby decreasing its activity.
2. **Competitive Inhibition:** An inhibitor molecule structurally similar to the enzyme's substrate binds to the active site, preventing the substrate from binding and hindering the catalytic process.
3. **Non-competitive Inhibition:** The inhibitor molecule binds to a site on the enzyme that is not the active site. This binding alters the enzyme's conformation and reduces its catalytic activity.
4. **Feedback Inhibition:** The product of a metabolic pathway acts as an inhibitor of an enzyme involved in an earlier step of the pathway. This mechanism prevents the overproduction of the product and regulates the flow of the metabolic pathway.
5. **Post-translational Modification:** Modifications to the enzyme, such as phosphorylation or ubiquitination, can influence its activity and stability. These modifications can be triggered by cellular signals and modulate the lyase enzyme's ability to catalyze reactions.

In addition to these general mechanisms, specific regulatory pathways exist for individual lyases. For example, the activity of pyruvate dehydrogenase, a key lyase involved in glucose metabolism, is negatively regulated by phosphorylation.

The negative regulation of lyase activity is essential for proper cellular function. By controlling the activity of these enzymes, cells can fine-tune their metabolic processes, maintain homeostasis, and respond to environmental changes.'
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Proteins (1)

ProteinDefinitionTaxonomy
14 kDa phosphohistidine phosphataseA 14 kDa phosphohistidine phosphatase that is encoded in the genome of human. [PRO:DNx, UniProtKB:Q9NRX4]Homo sapiens (human)

Compounds (6)

CompoundDefinitionClassesRoles
acetarsolacetamides;
anilide
ethacrynic acidetacrynic acid : An aromatic ether that is phenoxyacetic acid in which the phenyl ring is substituted by chlorines at positions 2 and 3, and by a 2-methylidenebutanoyl group at position 4. It is a loop diuretic used to treat high blood pressure resulting from diseases such as congestive heart failure, liver failure, and kidney failure. It is also a glutathione S-transferase (EC 2.5.1.18) inhibitor.

Ethacrynic Acid: A compound that inhibits symport of sodium, potassium, and chloride primarily in the ascending limb of Henle, but also in the proximal and distal tubules. This pharmacological action results in excretion of these ions, increased urinary output, and reduction in extracellular fluid. This compound has been classified as a loop or high ceiling diuretic.
aromatic ether;
aromatic ketone;
dichlorobenzene;
monocarboxylic acid
EC 2.5.1.18 (glutathione transferase) inhibitor;
ion transport inhibitor;
loop diuretic
ethylmaleimideEthylmaleimide: A sulfhydryl reagent that is widely used in experimental biochemical studies.maleimidesanticoronaviral agent;
EC 1.3.1.8 [acyl-CoA dehydrogenase (NADP(+))] inhibitor;
EC 2.1.1.122 [(S)-tetrahydroprotoberberine N-methyltransferase] inhibitor;
EC 2.7.1.1 (hexokinase) inhibitor
roxarsoneroxarsone : An organoarsonic acid where the organyl group is 4-hydroxy-3-nitrophenyl.

Roxarsone: An arsenic derivative which has anticoccidial action and promotes growth in animals.
2-nitrophenols;
organoarsonic acid
agrochemical;
animal growth promotant;
antibacterial drug;
coccidiostat
stictic acidstictic acid: antioxidant from lichen, Usnea articulata; structure in first sourcearomatic ether
norstictic acidnorstictic acid: from Xanthoparmelia chlorochroa; structure in first source