Protein arginine N-methyltransferase 5

A protein arginine N-methyltransferase 5 that is encoded in the genome of human. [PRO:DNx, UniProtKB:O14744]

Synonyms

PRMT5;
EC 2.1.1.320;
72 kDa ICln-binding protein;
Histone-arginine N-methyltransferase PRMT5;
Jak-binding protein 1;
Shk1 kinase-binding protein 1 homolog;
SKB1 homolog;
SKB1Hs

Research

Bioassay Publications (15)

Timeframe	Studies on this Protein(%)	All Drugs %
pre-1990	0 (0.00)	18.7374
1990's	0 (0.00)	18.2507
2000's	0 (0.00)	29.6817
2010's	11 (73.33)	24.3611
2020's	4 (26.67)	2.80

Compounds (12)

Drugs with Inhibition Measurements

Drug	Taxonomy	Measurement	Average (mM)	Bioassay(s)	Publication(s)
pentamidine	Homo sapiens (human)	IC50	582.0000	1	1
hexamidine	Homo sapiens (human)	IC50	357.0000	1	1
sinefungin	Homo sapiens (human)	IC50	8.6000	1	1
furamidine	Homo sapiens (human)	IC50	211.0000	2	2
s-adenosylhomocysteine	Homo sapiens (human)	IC50	12.8400	4	4
stilbamidine	Homo sapiens (human)	IC50	378.0500	2	2
epz004777	Homo sapiens (human)	IC50	20.0000	1	1
gsk343	Homo sapiens (human)	IC50	100.0000	1	1
6,7-dimethoxy-2-(pyrrolidin-1-yl)-n-(5-(pyrrolidin-1-yl)pentyl)quinazolin-4-amine	Homo sapiens (human)	IC50	100.0000	1	1
gsk3235025	Homo sapiens (human)	IC50	8.3678	12	12

Drugs with Activation Measurements

Drug	Taxonomy	Measurement	Average (mM)	Bioassay(s)	Publication(s)
s-adenosylmethionine	Homo sapiens (human)	Kd	0.5500	1	1
5'-methylthioadenosine	Homo sapiens (human)	Kd	0.6610	1	1
gsk3235025	Homo sapiens (human)	Kd	11.0000	1	1

Enables

This protein enables 15 target(s):

Target	Category	Definition
p53 binding	molecular function	Binding to one of the p53 family of proteins. [GOC:hjd]
transcription corepressor activity	molecular function	A transcription coregulator activity that represses or decreases the transcription of specific gene sets via binding to a DNA-bound DNA-binding transcription factor, either on its own or as part of a complex. Corepressors often act by altering chromatin structure and modifications. For example, one class of transcription corepressors modifies chromatin structure through covalent modification of histones. A second class remodels the conformation of chromatin in an ATP-dependent fashion. A third class modulates interactions of DNA-bound DNA-binding transcription factors with other transcription coregulators. [GOC:txnOH-2018, PMID:10213677, PMID:16858867]
protein binding	molecular function	Binding to a protein. [GOC:go_curators]
methyltransferase activity	molecular function	Catalysis of the transfer of a methyl group to an acceptor molecule. [ISBN:0198506732]
methyl-CpG binding	molecular function	Binding to a methylated cytosine/guanine dinucleotide. [GOC:jl, PMID:11746232]
protein-arginine N-methyltransferase activity	molecular function	Catalysis of the reaction: S-adenosyl-L-methionine + (protein)-arginine = S-adenosyl-L-homocysteine + (protein)-N-methyl-arginine. [GOC:mah, PMID:12351636, PMID:31284549]
protein-arginine omega-N symmetric methyltransferase activity	molecular function	Catalysis of the addition of a second methyl group to methylated peptidyl-arginine. Methylation is on the terminal nitrogen (omega nitrogen) residue that is not already methylated, resulting in symmetrical peptidyl-N(omega),N'(omega)-dimethyled arginine residues. [EC:2.1.1.320, PMID:14705965, RESID:AA0067, RESID:AA0069]
histone methyltransferase activity	molecular function	Catalysis of the reaction: S-adenosyl-L-methionine + histone = S-adenosyl-L-homocysteine + methyl-histone. Histone methylation generally occurs on either an arginine or a lysine residue. [GOC:curators]
identical protein binding	molecular function	Binding to an identical protein or proteins. [GOC:jl]
ribonucleoprotein complex binding	molecular function	Binding to a complex of RNA and protein. [GOC:bf, GOC:go_curators, GOC:vk]
histone H4R3 methyltransferase activity	molecular function	Catalysis of the reaction: S-adenosyl-L-methionine + (histone H4)-arginine (position 3) = S-adenosyl-L-homocysteine + (histone H4)-N-methyl-arginine (position 3). This reaction is the addition of a methyl group to the arginine residue at position 3 of histone H4, producing histone H4R3me. [GOC:mah, PMID:17898714]
protein heterodimerization activity	molecular function	Binding to a nonidentical protein to form a heterodimer. [GOC:ai]
E-box binding	molecular function	Binding to an E-box, a DNA motif with the consensus sequence CANNTG that is found in the promoters of a wide array of genes expressed in neurons, muscle and other tissues. [GOC:BHF, GOC:vk, PMID:11812799]
histone H3 methyltransferase activity	molecular function	Catalysis of the reaction: S-adenosyl-L-methionine + a histone H3 = S-adenosyl-L-homocysteine + a methylated histone H3. Histone methylation generally occurs on either an arginine or a lysine residue. [PMID:28450737]
histone arginine N-methyltransferase activity	molecular function	Catalysis of the reaction: S-adenosyl-L-methionine + (histone)-arginine = S-adenosyl-L-homocysteine + (histone)-N-methyl-arginine. [PMID:8002954]

Located In

This protein is located in 5 target(s):

Target	Category	Definition
nucleus	cellular component	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. [GOC:go_curators]
nucleoplasm	cellular component	That part of the nuclear content other than the chromosomes or the nucleolus. [GOC:ma, ISBN:0124325653]
Golgi apparatus	cellular component	A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways. [ISBN:0198506732]
cytosol	cellular component	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. [GOC:hjd, GOC:jl]
cytoplasm	cellular component	The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. [ISBN:0198547684]

Active In

This protein is active in 2 target(s):

Target	Category	Definition
cytosol	cellular component	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. [GOC:hjd, GOC:jl]
nucleus	cellular component	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. [GOC:go_curators]

Part Of

This protein is part of 3 target(s):

Target	Category	Definition
methylosome	cellular component	A large (20 S) protein complex that possesses protein arginine methyltransferase activity and modifies specific arginines to dimethylarginines in the arginine- and glycine-rich domains of several spliceosomal Sm proteins, thereby targeting these proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein (snRNP) core particles. Proteins found in the methylosome include the methyltransferase JBP1 (PRMT5), pICln (CLNS1A), MEP50 (WDR77), and unmethylated forms of SM proteins that have RG domains. [PMID:11713266, PMID:11756452]
chromatin	cellular component	The ordered and organized complex of DNA, protein, and sometimes RNA, that forms the chromosome. [GOC:elh, PMID:20404130]
histone methyltransferase complex	cellular component	A multimeric complex that is able to catalyze the addition of methyl groups to histone proteins. [GOC:bf]

Involved In

This protein is involved in 18 target(s):

Target	Category	Definition
spliceosomal snRNP assembly	biological process	The aggregation, arrangement and bonding together of one or more snRNA and multiple protein components to form a ribonucleoprotein complex that is involved in formation of the spliceosome. [GOC:krc, GOC:mah, ISBN:0879695897]
chromatin remodeling	biological process	A dynamic process of chromatin reorganization resulting in changes to chromatin structure. These changes allow DNA metabolic processes such as transcriptional regulation, DNA recombination, DNA repair, and DNA replication. [GOC:jid, GOC:vw, PMID:12042764, PMID:12697820]
DNA-templated transcription termination	biological process	The completion of transcription: the RNA polymerase pauses, the RNA-DNA hybrid dissociates, followed by the release of the RNA polymerase from its DNA template. [GOC:txnOH, PMID:15020047, PMID:18280161, PMID:30978344]
regulation of mitotic nuclear division	biological process	Any process that modulates the frequency, rate or extent of mitosis. [GOC:go_curators]
peptidyl-arginine methylation	biological process	The addition of a methyl group to an arginine residue in a protein. [GOC:mah]
circadian regulation of gene expression	biological process	Any process that modulates the frequency, rate or extent of gene expression such that an expression pattern recurs with a regularity of approximately 24 hours. [GOC:mah]
endothelial cell activation	biological process	The change in morphology and behavior of an endothelial cell resulting from exposure to a cytokine, chemokine, cellular ligand, or soluble factor. [GOC:mgi_curators, ISBN:0781735149, PMID:12851652, PMID:14581484]
negative regulation of gene expression via chromosomal CpG island methylation	biological process	An epigenetic gene regulation mechanism that negatively regulates gene expression by methylation of cytosine residues in chromosomal CpG islands. CpG islands are genomic regions that contain a high frequency of the CG dinucleotide associated with the transcription start site of genes. [PMID:11898023, Wikipedia:Cpg_island]
negative regulation of cell differentiation	biological process	Any process that stops, prevents, or reduces the frequency, rate or extent of cell differentiation. [GOC:go_curators]
negative regulation of DNA-templated transcription	biological process	Any process that stops, prevents, or reduces the frequency, rate or extent of cellular DNA-templated transcription. [GOC:go_curators, GOC:txnOH]
positive regulation of mRNA splicing, via spliceosome	biological process	Any process that activates or increases the rate or extent of mRNA splicing via a spliceosomal mechanism. [GOC:jid]
positive regulation of oligodendrocyte differentiation	biological process	Any process that activates or increases the frequency, rate or extent of oligodendrocyte differentiation. [GOC:vp, PMID:15139015]
regulation of ERK1 and ERK2 cascade	biological process	Any process that modulates the frequency, rate or extent of signal transduction mediated by the ERK1 and ERK2 cascade. [GOC:add, ISBN:0121245462, ISBN:0896039986]
Golgi ribbon formation	biological process	The formation of a continuous ribbon of interconnected Golgi stacks of flat cisternae. [GOC:ascb_2009, GOC:dph, GOC:tb]
liver regeneration	biological process	The regrowth of lost or destroyed liver. [GOC:gap, PMID:19447520]
regulation of signal transduction by p53 class mediator	biological process	Any process that modulates the frequency, rate or extent of signal transduction by p53 class mediator. [GOC:TermGenie]
positive regulation of adenylate cyclase-inhibiting dopamine receptor signaling pathway	biological process	Any process that activates or increases the frequency, rate or extent of adenylate cyclase-inhibiting dopamine receptor signaling pathway. [GO_REF:0000058, GOC:kmv, GOC:TermGenie, PMID:26554819]
regulation of DNA-templated transcription	biological process	Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription. [GOC:go_curators, GOC:txnOH]