biotin has been researched along with sepharose in 85 studies
| Studies (biotin) | Trials (biotin) | Recent Studies (post-2010) (biotin) | Studies (sepharose) | Trials (sepharose) | Recent Studies (post-2010) (sepharose) |
|---|---|---|---|---|---|
| 14,847 | 95 | 3,882 | 7,485 | 10 | 1,669 |
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 18 (21.18) | 18.7374 |
| 1990's | 25 (29.41) | 18.2507 |
| 2000's | 25 (29.41) | 29.6817 |
| 2010's | 15 (17.65) | 24.3611 |
| 2020's | 2 (2.35) | 2.80 |
| Authors | Studies |
|---|---|
| Inoue, H; Lowenstein, JM | 1 |
| Swack, JA; Utter, MF; Zander, GL | 1 |
| Landman, AD; Landman, NN | 1 |
| Berger, M; Wood, HG | 1 |
| Hagiwara, H; Hirose, S; Ito, T; Kozuka, M; Lodhi, KM; Nagasawa, T | 1 |
| Geahlen, RL; Lloyd, D; Loudon, GM; Paige, LA | 1 |
| Overdijk, B; Van Steijn, GJ | 1 |
| Bayer, EA; Wilchek, M | 1 |
| Gontijo, NF; Pena, SD; Ribeiro, JC | 1 |
| Kohanski, RA; Lane, MD | 3 |
| Battistin, L; Bracco, F; Gallo, P; Tavolato, B | 1 |
| Torres, AR; Ward, DC; Welcher, AA | 1 |
| Folsom, V; Haces, A; Harding, JD; Hunkeler, MJ | 1 |
| Hultman, T; Moks, T; Olsson, A; Ståhl, S; Uhlén, M | 1 |
| Nicolson, GL; Updyke, TV | 1 |
| Heney, GC; Orr, GA; Zeheb, R | 1 |
| Chi, T; Cone, RE | 1 |
| Bayer, E; Wilchek, M | 1 |
| Kostulas, V; Link, H; Olsson, T | 1 |
| Harmon, FR | 1 |
| Lenhoff, HM; Ngo, TT | 1 |
| Heney, G; Orr, GA | 1 |
| Vaughan, L; Weber, P; Winterhalter, KH; Zimmermann, DR | 1 |
| Caplan, MJ; Dunbar, LA; Gottardi, CJ | 1 |
| Fahrenholz, F; Thiele, C | 1 |
| Florin, EL; Gaub, HE; Moy, VT | 1 |
| Glad, G; Kwiatkowski, M; Lagerkvist, A; Lagerström Fermér, M; Landegren, U; Mendel-Hartvig, M; Parik, J; Samiotaki, M; Stewart, J | 1 |
| Haase, FC; Kumar, GK; Magner, WJ; Phillips, NF; Samols, D; Shenoy, BC | 1 |
| Beer, DJ; Jack, GW; Randles, SC; Yates, AM | 1 |
| Casida, JE; Li, QX | 1 |
| Lucius, R; Ludwig, R; Mentlein, R | 1 |
| Abrams, WR; Czerwinski, M; Siegel, DL; Spitalnik, SL; Stwora-Wojczyk, MM; Wojczyk, BS; Wunner, WH | 1 |
| Balsera, M; Izrailev, S; Oono, Y; Schulten, K; Stepaniants, S | 1 |
| Airenne, KJ; Bayer, EA; Kulomaa, MS; Marjomäki, VS; Oker-Blom, C; Wilchek, M | 1 |
| Adams, S; McGrath, P; Weaver, JC | 1 |
| Fan, J; Huennekens, FM | 1 |
| Loos, U; Minich, WB; Weymayer, JD | 1 |
| Bennett, CA; Easterbrook-Smith, SB; Pankhurst, GJ | 1 |
| Fujiwara, H; Fujiwara, T; Hayakawa, Y; Kanoh, M; Minatoguchi, S; Misao, J; Ohno, A; Ohno, M; Takemura, G; Uno, Y | 1 |
| Haehnel, W; Hörth, P; Mühlenhoff, U; Schilz, E; von Leoprechting, A | 1 |
| Hillmann, T; Jiao, Y; Lehmann, H; Moy, VT; Sano, T | 1 |
| Bolognia, SL; Hennes, JL; Mascotti, DP; Patel, P; Rodgers, JT | 1 |
| Bosch, T; Duhr, C; Fink, E; Lennertz, A; Samtleben, W | 1 |
| Bonzagni, NJ; Murakami, H; Suga, H | 1 |
| Grunnet, M; Jespersen, T; Jorgensen, NK; Klaerke, DA; Ljungstrøm, T; Olesen, SP; Rasmussen, HB | 1 |
| Baumeister, S; Hibbs, AR; Lingelbach, K; Nyalwidhe, J; Papakrivos, J; Tawill, S; Volker, U | 1 |
| Beechem, JM; Eslamizar, L; Filanoski, BJ; Haugland, RP; Hirsch, JD; Malekzadeh, N | 1 |
| Moy, VT; Zhang, X | 1 |
| Bigler, N; Demotz, S; Denis, B; Moulon, C; Perrenoud, G; Pinaud, S; Roggero, M; Thierry, AC | 1 |
| Hoja, U; Kim, HS; Sauer, G; Schweizer, E; Stolz, J | 1 |
| Conrads, TP; Hood, BL; Kim, BJ; Song, BJ; Suh, SK; Veenstra, TD | 1 |
| Wong, SL; Wu, SC | 1 |
| Beish, L; Davidson, WS; Ghering, AB; Hui, DY; Pearson, K; Tubb, MR | 1 |
| Aragon, RA; Conrads, TP; Hardwick, JP; Hood, BL; Kim, BJ; Song, BJ; Veenstra, TD | 1 |
| Bonte, E; Braun, H; Campbell, J; de Boer, E; Grosveld, F; Kolodziej, KE; Philipsen, S; Rodriguez, P; Strouboulis, J | 1 |
| Hilty, C; Lowery, TJ; Pines, A; Schröder, L; Wemmer, DE | 1 |
| Liu, BC; Lu, ZH; Lv, LL; Tang, ZM; Zhang, CX; Zhang, L | 1 |
| Haystead, TA; MacKintosh, C; Moorhead, GB | 1 |
| Bhuniya, S; Kim, BH; Seo, YJ | 1 |
| Choi, NS; Dang, Y; Li, S; Liu, JO; Low, WK; Ma, G; Park, K; Romo, D; Rzasa, RM; Schneider-Poetsch, T; Shea, HA; Shi, Z | 1 |
| Hayashi, Y; Kiso, Y; Yuri, Y | 1 |
| Chodosh, LA | 1 |
| Creager, SE; Rao, AK | 1 |
| Haishima, Y; Nakagawa, K; Nakamura, K; Ogawa, H; Saito, K; Sakagami, H; Yamagami, M | 1 |
| Goldar, A; Hyrien, O; Krude, T; Marheineke, K | 1 |
| Demczuk, A; Norio, P | 1 |
| Früh, V; IJzerman, AP; Siegal, G | 1 |
| Collins, AR; Shaposhnikov, S; Thomsen, PD | 1 |
| Wang, JY; Xiao, L | 1 |
| Bau, HH; Thompson, JA | 1 |
| Ahsan, S; Aizawa, Y; Maxwell, KL; Morshead, CM; Shoichet, MS; Wylie, RG | 1 |
| Blom, E; Carlsson, F; Eriksson, O; Korsgren, O; Långström, B; Sundin, A; Velikyan, I | 1 |
| Byun, JY; Kim, BG; Kim, DM; Lee, KH; Lee, KY | 1 |
| Gu, C; Lu, X; Pan, X; Quan, B; Wang, L; Wu, X; Xu, X | 1 |
| Cardamone, D; Master, SR; Rulander, NJ; Senior, M; Snyder, PJ | 1 |
| Kashyap, P; Lam, C; Pavel, MA; Salehi-Najafabadi, Z; Singh, G; Yu, Y | 1 |
| Crupi, MJ; Mulligan, LM; Richardson, DS | 1 |
| Peng, CA; Salehi, N | 1 |
| Huynh, V; Wylie, RG | 1 |
| Huynh, V; Lambert, CR; Nijsure, D; Wylie, RG | 1 |
| Hwang, SH; Kim, CH; Lee, MK; Lee, SJ; Park, YM; Song, Y | 1 |
| Gingras, AC; Larsen, B; Raught, B; Samavarchi Tehrani, P; St-Germain, JR; Wong, C | 1 |
| Aoyama, T; Hamachi, I; Kubota, R; Matsuzaki, T; Minami, S; Nakamura, K; Shigemitsu, H; Urayama, K | 1 |
3 review(s) available for biotin and sepharose
| Article | Year |
|---|---|
Isolation and identification of eukaryotic initiation factor 4A as a molecular target for the marine natural product Pateamine A.
Topics: Animals; Bacterial Proteins; Biotin; Chromatography, Affinity; Cyclohexylamines; Drug Design; Epoxy Compounds; Eukaryotic Initiation Factor-4A; Humans; Macrolides; Models, Biological; Protein Binding; Sepharose; Structure-Activity Relationship; Thiazoles | 2007 |
[An approach to the targeted attachment of peptides and proteins to solid supports].
Topics: Adrenocorticotropic Hormone; Amino Acid Sequence; Avidin; Biotin; Biotinylation; Chemistry, Organic; Chromatography, Affinity; Organic Chemistry Phenomena; Peptides; Receptors, Peptide; Sepharose | 2007 |
How to catch a membrane protein in action: a review of functional membrane protein immobilization strategies and their applications.
Topics: Biotin; Carbodiimides; Detergents; Humans; Immobilized Proteins; Lipid Bilayers; Membrane Proteins; Sepharose; Wheat Germ Agglutinins | 2011 |
82 other study(ies) available for biotin and sepharose
| Article | Year |
|---|---|
Acetyl coenzyme A carboxylase from rat liver. EC 6.4.1.2 acetyl-CoA: carbon dioxide ligase (ADP).
Topics: Acetyl-CoA Carboxylase; Animals; Biotin; Chromatography, DEAE-Cellulose; Chromatography, Gel; Coenzyme A; Enzyme Activation; Ligases; Liver; Methods; Molecular Weight; Palmitic Acids; Rats; Sepharose; Species Specificity | 1975 |
Use of avidin-sepharose to isolate and idenify biotin polypeptides from crude extracts.
Topics: Animals; Avidin; Biotin; Chickens; Chromatography, Affinity; Mitochondria, Liver; Ovalbumin; Peptides; Protein Binding; Pyruvate Carboxylase; Rats; Sepharose | 1978 |
The binding of biotin to sepharose-avidin column. Demonstration of the affinity chromatography technique.
Topics: Avidin; Biotin; Chromatography, Affinity; Polysaccharides; Sepharose | 1976 |
Purification of the subunits of transcarboxylase by affinity chromatography on avidin-sepharose.
Topics: Avidin; Binding Sites; Biotin; Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Macromolecular Substances; Malonates; Propionibacterium; Protein Binding; Pyruvates; Sepharose; Transferases; Ultracentrifugation | 1975 |
Affinity chromatographic purification of bovine lung endothelin receptor using biotinylated endothelin and avidin-agarose.
Topics: Amino Acid Sequence; Animals; Avidin; Biotin; Cattle; Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Endothelins; Lung; Molecular Sequence Data; Receptors, Cell Surface; Receptors, Endothelin; Sepharose | 1992 |
A general method for preparation of peptides biotinylated at the carboxy terminus.
Topics: Amino Acid Sequence; Avidin; Biotin; Chromatography, Affinity; Chromatography, High Pressure Liquid; Lysine; Molecular Sequence Data; Oligopeptides; Sepharose | 1992 |
Binding of lysosomal enzymes to the mannose 6-phosphate receptor: a novel binding assay that makes use of biotinylated receptor molecules, coupled to avidin-agarose.
Topics: Animals; Avidin; beta-Galactosidase; beta-N-Acetylhexosaminidases; Biotin; Cattle; Chromatography, Affinity; Hydrogen-Ion Concentration; Kidney; Liver; Lysosomes; Receptor, IGF Type 2; Receptors, Cell Surface; Sepharose | 1990 |
Avidin column as a highly efficient and stable alternative for immobilization of ligands for affinity chromatography.
Topics: Antibodies; Avidin; Biotin; Chromatography, Affinity; Lectins; Ligands; Sepharose | 1990 |
Direct hybridization of biotin-labeled probes in dried agarose gels.
Topics: Biotin; DNA Probes; Humans; Nucleic Acid Hybridization; Sepharose | 1990 |
Monovalent avidin affinity columns.
Topics: Avidin; Biotin; Chromatography, Affinity; Indicators and Reagents; Protein Binding; Sepharose | 1990 |
Detection of IgG oligoclonal bands in unconcentrated CSF by means of agarose isoelectric focusing, double immunofixation peroxidase staining and avidin-biotin amplification.
Topics: Avidin; Biotin; Humans; Immunoenzyme Techniques; Immunoglobulins; Isoelectric Focusing; Monoclonal Gammopathy of Undetermined Significance; Multiple Sclerosis; Nervous System Diseases; Oligoclonal Bands; Ovalbumin; Sepharose; Staining and Labeling | 1985 |
Selective enrichment of specific DNA, cDNA and RNA sequences using biotinylated probes, avidin and copper-chelate agarose.
Topics: Avidin; Base Sequence; Biotin; Cell Line; Chelating Agents; Copper; DNA; DNA, Bacterial; Escherichia coli; Humans; Imino Acids; Indicators and Reagents; Neisseria gonorrhoeae; Neisseria meningitidis; Poly A; RNA; RNA, Messenger; Sepharose | 1986 |
Detection of DNA targets with biotinylated and fluoresceinated RNA probes. Effects of the extent of derivitization on detection sensitivity.
Topics: Adenosine Monophosphate; Adenosine Triphosphate; Biotin; Chromatography, Thin Layer; DNA; Fluoresceins; Humans; Immunoblotting; RNA; RNA Probes; Sepharose | 1989 |
Solid phase DNA sequencing using the biotin-avidin system.
Topics: Autoanalysis; Avidin; Base Sequence; Biotin; Cloning, Molecular; Deoxyribonucleotides; DNA; Enzymes, Immobilized; Genetic Vectors; Plasmids; Sepharose; Sodium Hydroxide | 1988 |
Immunoaffinity isolation of membrane antigens with biotinylated monoclonal antibodies and streptavidin-agarose.
Topics: Animals; Antibodies, Monoclonal; Antigen-Antibody Complex; Antigens, Surface; Bacterial Proteins; Biotin; Carbon Radioisotopes; Cell Membrane; Chromatography, Affinity; Indicators and Reagents; Mice; Radioisotope Dilution Technique; Rats; Sepharose | 1986 |
Purification of avidin and its derivatives on 2-iminobiotin-6-aminohexyl-Sepharose 4B.
Topics: Avidin; Biotin; Chromatography, Affinity; Indicators and Reagents; Ovalbumin; Sepharose | 1986 |
Receptor affinity chromatography.
Topics: Adipose Tissue; Animals; Avidin; Biotin; Chromatography, Affinity; In Vitro Techniques; Mice; Ovalbumin; Receptor, Insulin; Sepharose | 1985 |
Homogeneous functional insulin receptor from 3T3-L1 adipocytes. Purification using N alpha B1-(biotinyl-epsilon-aminocaproyl)insulin and avidin-sepharose.
Topics: Adipose Tissue; Animals; Avidin; Biotin; Cell Line; Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Hydrogen-Ion Concentration; Insulin; Mice; Protein Kinases; Receptor, Insulin; Sepharose | 1985 |
Cell sorting of lymphocyte subpopulations by biotinylated antibody and avidin-Sepharose beads.
Topics: Animals; Antilymphocyte Serum; Avidin; Biotin; Cell Separation; Female; Fluorescent Antibody Technique; Lymphocytes; Mice; Mice, Inbred C57BL; Mice, Inbred CBA; Sepharose; Spleen | 1985 |
Insolubilized biotin for the purification of avidin.
Topics: Avidin; Biotin; Chromatography, Affinity; Methods; Ovalbumin; Protein Binding; Sepharose | 1974 |
Improved detection of oligoclonal IgG in cerebrospinal fluid by isoelectric focusing in agarose, double-antibody peroxidase labeling, and avidin-biotin amplification.
Topics: Avidin; Biotin; Central Nervous System Diseases; Collodion; Fluorescent Antibody Technique; Humans; Immunoenzyme Techniques; Immunoglobulin G; Indicators and Reagents; Infections; Isoelectric Focusing; Multiple Sclerosis; Rosaniline Dyes; Sepharose | 1984 |
Purification of antibodies against biotin of lipoic acid-Sepharose.
Topics: Antibodies; Antibody Affinity; Biotin; Carboxyl and Carbamoyl Transferases; Chromatography, Affinity; Drug Stability; Methylmalonic Acid; Sepharose; Thioctic Acid; Transferases | 1980 |
New approach to heterogeneous enzyme immunoassays using tagged enzyme-ligand conjugates.
Topics: Antibodies; Antigen-Antibody Complex; Avidin; beta-Galactosidase; Biotin; Escherichia coli; Galactosidases; Immunoenzyme Techniques; Indicators and Reagents; Ligands; Sepharose | 1981 |
The purification of avidin and its derivatives on 2-iminobiotin-6-aminohexyl-Sepharose 4B.
Topics: Avidin; Biotin; Carbon Radioisotopes; Chromatography, Affinity; Ovalbumin; Rhodamines; Sepharose; Tritium | 1981 |
Tenascin-C binds heparin by its fibronectin type III domain five.
Topics: Amino Acid Sequence; Animals; Biotin; Cattle; Cell Adhesion Molecules, Neuronal; DNA, Recombinant; Extracellular Matrix Proteins; Fibronectins; Glycosaminoglycans; Heparin; Models, Molecular; Molecular Sequence Data; Protein Binding; Recombinant Fusion Proteins; Sepharose; Sharks; Swine; Tenascin | 1995 |
Biotinylation and assessment of membrane polarity: caveats and methodological concerns.
Topics: Animals; Bacterial Proteins; Biotin; Buffers; Cell Line; Cell Membrane; Cell Polarity; Dogs; Edetic Acid; Filtration; Hemagglutinins; Hydrogen-Ion Concentration; Kidney; LLC-PK1 Cells; Methods; Precipitin Tests; Sepharose; Streptavidin; Swine | 1995 |
Photocleavable biotinylated ligands for affinity chromatography.
Topics: Amino Acid Sequence; Animals; Bacterial Proteins; Biotin; Cholecystokinin; Chromatography, Affinity; Ligands; Light; Molecular Sequence Data; Photochemistry; Rabbits; Receptors, Cholecystokinin; Sepharose; Streptavidin | 1994 |
Adhesion forces between individual ligand-receptor pairs.
Topics: Adhesiveness; Avidin; Biotin; Ligands; Microscopy; Microspheres; Receptors, Cell Surface; Sepharose | 1994 |
A manifold support for molecular genetic reactions.
Topics: Avidin; Bacterial Proteins; Biotin; DNA; Kinetics; Molecular Biology; Polymerase Chain Reaction; Polystyrenes; Sepharose; Streptavidin | 1993 |
The nonbiotinylated form of the 1.3 s subunit of transcarboxylase binds to avidin (monomeric)-agarose: purification and separation from the biotinylated 1.3 S subunit.
Topics: Amino Acid Sequence; Amino Acids; Avidin; Biotin; Carboxyl and Carbamoyl Transferases; Chromatography, Affinity; Chromatography, High Pressure Liquid; Cloning, Molecular; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Molecular Sequence Data; Propionibacterium; Sepharose; Transferases | 1993 |
A comparison of the leakage of a monoclonal antibody from various immunoaffinity chromatography matrices.
Topics: Animals; Antibodies, Monoclonal; Biotin; Chromatography, Affinity; Cyanogen Bromide; Enzyme-Linked Immunosorbent Assay; Evaluation Studies as Topic; Follicle Stimulating Hormone; Follicle Stimulating Hormone, beta Subunit; Humans; Imidazoles; Immunosorbent Techniques; Mice; Rats; Sepharose; Thyrotropin | 1995 |
Affinity probes for the GABA-gated chloride channel: 5e-tert-butyl-2e-[4-(substituted-ethynyl)phenyl]-1,3-dithianes with photoactivatable, fluorescent, biotin, agarose and protein substituents.
Topics: Affinity Labels; Animals; Binding Sites; Biotin; Brain; Cattle; Chloride Channels; Fluorescent Dyes; gamma-Aminobutyric Acid; Heterocyclic Compounds; Immunochemistry; In Vitro Techniques; Ion Channel Gating; Magnetic Resonance Spectroscopy; Molecular Structure; Photochemistry; Proteins; Rabbits; Sepharose | 1995 |
A radioactive assay for the degradation of neuropeptide Y.
Topics: Amino Acid Sequence; Animals; Bacterial Proteins; Biotin; Chloramines; Endopeptidases; Fibrinolysin; Iodine Radioisotopes; Molecular Sequence Data; Neuropeptide Y; Radioligand Assay; Sepharose; Streptavidin; Tosyl Compounds; Trypsin | 1995 |
Purification of a secreted form of recombinant rabies virus glycoprotein: comparison of two affinity tags.
Topics: Affinity Labels; Amino Acid Sequence; Animals; Antigens, Viral; Bacterial Proteins; Base Sequence; Biotin; Blotting, Western; CHO Cells; Chromatography, Affinity; Cricetinae; Electrophoresis, Polyacrylamide Gel; Epitopes; Gene Expression Regulation; Glycoproteins; Histidine; Molecular Sequence Data; Recombinant Fusion Proteins; Sepharose; Sequence Analysis; Streptavidin; Transfection; Viral Envelope Proteins | 1996 |
Molecular dynamics study of unbinding of the avidin-biotin complex.
Topics: Avidin; Binding Sites; Biotin; Computer Simulation; Hydrogen Bonding; Mathematics; Microscopy, Atomic Force; Microspheres; Models, Molecular; Molecular Structure; Protein Binding; Protein Conformation; Protein Folding; Sepharose | 1997 |
Production of biologically active recombinant avidin in baculovirus-infected insect cells.
Topics: Animals; Avidin; Baculoviridae; Biotin; Chickens; Chromatography, Affinity; Enzyme-Linked Immunosorbent Assay; Genetic Vectors; Glycosylation; Protein Conformation; Protein Denaturation; Recombinant Proteins; Sepharose; Spodoptera | 1997 |
Gel microdrop technology for rapid isolation of rare and high producer cells.
Topics: Antibodies, Monoclonal; Biotin; Cell Separation; Clone Cells; Flow Cytometry; Fluorescent Antibody Technique, Indirect; Gels; Humans; Indicators and Reagents; Recombinant Proteins; Sepharose | 1997 |
Biotin derivatives of folate compounds: synthesis and utilization for visualization and affinity purification of folate transport proteins.
Topics: Animals; Bacterial Proteins; Biological Transport; Biotin; Carrier Proteins; Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Fluorescein; Fluoresceins; Folate Receptors, GPI-Anchored; Folic Acid; Leukemia L1210; Methotrexate; Microscopy; Molecular Structure; Receptors, Cell Surface; Sepharose; Tumor Cells, Cultured | 1997 |
Expression of a biotinylated human thyrotropin receptor in HeLa cells using recombinant vaccinia virus and its application for the detection of Graves' autoantibodies.
Topics: Autoantibodies; Bacterial Proteins; Biotin; Cyclic AMP; Genetic Vectors; Graves Disease; HeLa Cells; Humans; Receptors, Thyrotropin; Recombinant Fusion Proteins; Recombination, Genetic; Second Messenger Systems; Sepharose; Thyrotropin; Vaccinia virus | 1998 |
Characterization of the heparin-binding properties of human clusterin.
Topics: Biotin; Calcium; Clusterin; Enzyme-Linked Immunosorbent Assay; Glycoproteins; Heparin; Humans; Hydrogen-Ion Concentration; Molecular Chaperones; Protein Binding; Sepharose | 1998 |
Role of apoptosis in the disappearance of infiltrated and proliferated interstitial cells after myocardial infarction.
Topics: Animals; Apoptosis; Biotin; Deoxyuracil Nucleotides; DNA; DNA Fragmentation; Electrophoresis, Agar Gel; Endothelium, Vascular; Leukocytes; Male; Microscopy, Electron; Muscle Fibers, Skeletal; Myocardial Infarction; Rabbits; Sepharose; Staining and Labeling; Wound Healing | 1998 |
Identification of biotinylation sites on proteins by selective retrieval of 2-iminobiotinylated peptides from proteolytic peptide mixtures: localization of the accessible lysine residues on the photosystem I subunits PsaD and PsaE.
Topics: Affinity Labels; Amino Acid Sequence; Avidin; Bacterial Proteins; Binding Sites; Biotin; Biotinylation; Chromatography, High Pressure Liquid; Cyanobacteria; Lysine; Mass Spectrometry; Molecular Sequence Data; Peptide Mapping; Photosynthetic Reaction Center Complex Proteins; Photosystem I Protein Complex; Plant Proteins; Proteins; Recombinant Proteins; Sepharose; Sequence Analysis; Serine Endopeptidases | 1998 |
Adhesion energy of receptor-mediated interaction measured by elastic deformation.
Topics: Adhesiveness; Avidin; Biophysical Phenomena; Biophysics; Biotin; Cell Adhesion; Elasticity; Microspheres; Models, Biological; Receptors, Cell Surface; Sepharose; Static Electricity; Surface Properties; Thermodynamics | 1999 |
Use of biotin-labeled nucleic acids for protein purification and agarose-based chemiluminescent electromobility shift assays.
Topics: Animals; Biological Assay; Biotin; Microspheres; Nucleic Acids; Proteins; Rats; Sepharose | 2000 |
Ex vivo biocompatibility of avidin-agarose: a new device for direct adsorption of biotinylated antibodies from human whole blood.
Topics: Adsorption; Antibodies; Antibodies, Neoplasm; Anticoagulants; Antithrombin III; Avidin; beta-Thromboglobulin; Biocompatible Materials; Biotin; Blood Cell Count; Bradykinin; Chromatography, Affinity; Citrates; Complement C3a; Complement C5a; Hemolysis; Hemoperfusion; Heparin; Humans; Ligands; Pancreatic Elastase; Peptide Hydrolases; Pressure; Radioimmunotherapy; Sepharose; Thrombosis | 2000 |
Aminoacyl-tRNA synthesis by a resin-immobilized ribozyme.
Topics: Biotin; Enzymes, Immobilized; Nucleic Acid Conformation; Phenylalanine; RNA, Catalytic; RNA, Transfer, Amino Acyl; Sepharose | 2002 |
KCNE4 is an inhibitory subunit to the KCNQ1 channel.
Topics: Animals; Biotin; Carrier Proteins; Cation Transport Proteins; CHO Cells; Cricetinae; DNA-Binding Proteins; Electrophoresis, Polyacrylamide Gel; Electrophysiology; ERG1 Potassium Channel; Ether-A-Go-Go Potassium Channels; Female; Immunoblotting; In Vitro Techniques; KCNQ Potassium Channels; KCNQ1 Potassium Channel; Membrane Potentials; Membrane Proteins; Mice; Microscopy, Confocal; Microscopy, Fluorescence; Oocytes; Patch-Clamp Techniques; Potassium Channels; Potassium Channels, Voltage-Gated; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Sepharose; Streptavidin; Tissue Distribution; Trans-Activators; Xenopus | 2002 |
A nonpermeant biotin derivative gains access to the parasitophorous vacuole in Plasmodium falciparum-infected erythrocytes permeabilized with streptolysin O.
Topics: Animals; Bacterial Proteins; Biotin; Biotinylation; Chromatography; Cytosol; Electrophoresis, Gel, Two-Dimensional; Erythrocytes; Fluorescent Antibody Technique, Indirect; Humans; Hydrogen-Ion Concentration; Immunoblotting; Plasmodium falciparum; Precipitin Tests; Protein Binding; Sepharose; Streptavidin; Streptolysins; Succinimides; Vacuoles | 2002 |
Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation.
Topics: Animals; Avidin; Biotin; Carrier Proteins; Chromatography, Affinity; Humans; Immunoglobulin G; Indicators and Reagents; Ligands; Protein Binding; Rabbits; Recombinant Proteins; Sepharose; Streptavidin; Tubulin | 2002 |
Cooperative adhesion of ligand-receptor bonds.
Topics: Adhesiveness; Biotin; Hydrogen-Ion Concentration; Ligands; Microscopy, Atomic Force; Protein Binding; Receptors, Cell Surface; Sepharose; Streptavidin | 2003 |
Biotinylated synthetic chemokines: their use for the development of nonradioactive whole-cell binding assays.
Topics: Biotin; Biotinylation; Cell Line; Cell Line, Tumor; Cell Movement; Cell Separation; Chemokine CCL22; Chemokines, CC; Dose-Response Relationship, Drug; Flow Cytometry; Humans; Ligands; Macrophages; Peptides; Protein Binding; Protein Folding; Receptors, CCR4; Receptors, Chemokine; Sepharose; Streptavidin; Time Factors; Transcription Factors; Transfection | 2003 |
Identification of the tRNA-binding protein Arc1p as a novel target of in vivo biotinylation in Saccharomyces cerevisiae.
Topics: Adenosine Triphosphate; Binding Sites; Biotin; Biotinylation; Blotting, Western; DNA Mutational Analysis; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Genetic Complementation Test; Genetic Vectors; Genotype; Glutamate-tRNA Ligase; Heterozygote; Lysine; Methionine-tRNA Ligase; Mutagenesis, Site-Directed; Mutation; Plasmids; Protein Binding; Protein Structure, Tertiary; RNA-Binding Proteins; RNA, Transfer; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Sepharose; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Streptavidin; Subcellular Fractions | 2004 |
Identification of oxidized mitochondrial proteins in alcohol-exposed human hepatoma cells and mouse liver.
Topics: Alcohol Drinking; Animals; Bacterial Proteins; Biotin; Carcinoma, Hepatocellular; Cysteine; Electrophoresis, Gel, Two-Dimensional; Ethanol; Humans; Liver; Mice; Mitochondria; Mitochondrial Proteins; Oxidation-Reduction; Sepharose; Sequence Analysis, Protein | 2004 |
Engineering soluble monomeric streptavidin with reversible biotin binding capability.
Topics: Bacillus subtilis; Binding Sites; Biotin; Blotting, Western; Chromatography, Gel; Cross-Linking Reagents; Electrophoresis, Polyacrylamide Gel; Endopeptidase K; Kinetics; Light; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Protein Binding; Protein Engineering; Protein Folding; Scattering, Radiation; Sepharose; Streptavidin; Surface Plasmon Resonance; Threonine; Valine | 2005 |
The biotin-capture lipid affinity assay: a rapid method for determining lipid binding parameters for apolipoproteins.
Topics: Algorithms; Apolipoprotein A-I; Apolipoprotein A-II; Apolipoproteins; Apolipoproteins A; Bacterial Proteins; Benzoates; Biotin; Chromatography, Affinity; Dansyl Compounds; Humans; Liposomes; Mutation; Phosphatidylcholines; Phosphatidylethanolamines; Phospholipids; Protein Binding; Quinolines; Recombinant Proteins; Reproducibility of Results; Rhodamines; Sepharose; Spectrometry, Fluorescence | 2006 |
Increased oxidation and degradation of cytosolic proteins in alcohol-exposed mouse liver and hepatoma cells.
Topics: Animals; Bacterial Proteins; Biomarkers, Tumor; Biotin; Carcinoma, Hepatocellular; Central Nervous System Depressants; Computational Biology; Cytochrome P-450 CYP2E1; Cytosol; Electrophoresis, Gel, Two-Dimensional; Ethanol; Liver; Liver Extracts; Liver Neoplasms; Male; Mice; Mice, Inbred C57BL; Oxidation-Reduction; Peroxidases; Peroxiredoxins; Sepharose; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tyrosine | 2006 |
Isolation of transcription factor complexes by in vivo biotinylation tagging and direct binding to streptavidin beads.
Topics: Amino Acid Sequence; Animals; Biotin; Blotting, Western; Carbon-Nitrogen Ligases; Cell Line; Chromatography, Agarose; Electrophoresis, Polyacrylamide Gel; Endopeptidases; Escherichia coli Proteins; GATA1 Transcription Factor; Mass Spectrometry; Mice; Molecular Sequence Data; Multiprotein Complexes; Proteomics; Repressor Proteins; Sepharose; Streptavidin; Transcription Factors | 2006 |
Molecular imaging using a targeted magnetic resonance hyperpolarized biosensor.
Topics: Avidin; Biosensing Techniques; Biotin; Contrast Media; Magnetic Resonance Imaging; Magnetic Resonance Spectroscopy; Microspheres; Sensitivity and Specificity; Sepharose; Time Factors; Xenon Isotopes | 2006 |
Construction of an antibody microarray based on agarose-coated slides.
Topics: Antibodies; Biotin; Carbocyanines; Chemokine CCL2; Immunoassay; Protein Array Analysis; Proteomics; Sepharose; Streptavidin | 2007 |
Synthesis and use of the protein phosphatase affinity matrices microcystin-sepharose and microcystin-biotin-sepharose.
Topics: Biotin; Chromatography, Affinity; Microcystins; Peptides, Cyclic; Phosphoprotein Phosphatases; Reproducibility of Results; Sepharose | 2007 |
Reversible sol-gel signaling system with epMB for the study of enzyme- and pH-triggered oligonucleotide release from a biotin hydrogel.
Topics: Acrylic Resins; Biotin; Chromatography, High Pressure Liquid; Fluorescence; Hydrogels; Hydrogen Bonding; Hydrogen-Ion Concentration; Leucine; Oligonucleotides; Pyrenes; Sepharose | 2007 |
Purification of DNA-binding proteins using biotin/streptavidin affinity systems.
Topics: Animals; Bacterial Proteins; Biotin; Biotinylation; Cellulose; Chromatography, Affinity; DNA; DNA-Binding Proteins; Humans; Protein Binding; Sepharose; Streptavidin | 2001 |
Preparation and characterization of superporous agarose-reticulated vitreous carbon electrodes as platforms for electrochemical bioassays.
Topics: Avidin; Binding Sites; Biological Assay; Biotin; Carbon; Electrochemistry; Electrodes; Microscopy, Confocal; Microscopy, Electron, Scanning; Porosity; Sepharose | 2008 |
Pseudoproteoglycan (pseudoPG) probes that simulate PG macromolecular structure for screening and isolation of PG-binding proteins.
Topics: Amino Acid Sequence; Animals; Biotin; Chromatography, Affinity; Chromatography, Gel; Kinetics; Light; Membranes, Artificial; Molecular Probes; Molecular Sequence Data; Protein Binding; Proteins; Proteoglycans; Rats; Scattering, Radiation; Sepharose; Surface Plasmon Resonance; Sus scrofa | 2009 |
Use of DNA combing to study DNA replication in Xenopus and human cell-free systems.
Topics: Animals; Biotin; Cell Line; Cell-Free System; Chromatin; Deoxyuracil Nucleotides; Digoxigenin; DNA Replication; Female; G1 Phase; HeLa Cells; Humans; In Situ Hybridization, Fluorescence; In Vitro Techniques; Male; Microscopy, Fluorescence; Ovum; Sepharose; Spermatozoa; Xenopus laevis | 2009 |
Determining the replication dynamics of specific gene loci by single-molecule analysis of replicated DNA.
Topics: Animals; Biotin; Cells, Cultured; Computational Biology; DNA; DNA Probes; DNA Replication; Electrophoresis, Gel, Pulsed-Field; Fluorescent Dyes; Genomics; Humans; In Situ Hybridization; In Situ Nick-End Labeling; Microscopy, Fluorescence; Nucleic Acid Conformation; Nucleic Acid Denaturation; Replication Origin; Sepharose; Silanes | 2009 |
Combining fluorescent in situ hybridization with the comet assay for targeted examination of DNA damage and repair.
Topics: Antibodies; Biotin; Cell Fractionation; Cell Line; Comet Assay; Digoxigenin; DNA Damage; DNA Probes; DNA Repair; Electrophoresis; Humans; In Situ Hybridization, Fluorescence; Nucleic Acid Denaturation; Sepharose; Solutions; Staining and Labeling | 2011 |
Posttranscriptional regulation of gene expression in epithelial cells by polyamines.
Topics: Animals; Antibodies; Biotin; Biotinylation; Blotting, Western; Cell Extracts; Cells, Cultured; DNA Probes; DNA, Complementary; Electrophoresis, Polyacrylamide Gel; Epithelial Cells; Gene Expression Regulation; Immunoprecipitation; Microspheres; Polyamines; Reverse Transcriptase Polymerase Chain Reaction; Ribonucleoproteins; RNA; Sepharose; Staphylococcal Protein A; Transcription, Genetic | 2011 |
Pulsating bead-based assay.
Topics: Biotin; Microfluidic Analytical Techniques; Particle Size; Porosity; Quantum Dots; Sepharose; Streptavidin; Surface Properties | 2011 |
Spatially controlled simultaneous patterning of multiple growth factors in three-dimensional hydrogels.
Topics: Bacterial Proteins; Biomimetic Materials; Biotin; Cell Culture Techniques; Ciliary Neurotrophic Factor; Hedgehog Proteins; Hydrogels; Intercellular Signaling Peptides and Proteins; Recombinant Fusion Proteins; Ribonucleases; Sepharose; Streptavidin; Tissue Scaffolds | 2011 |
Preclinical evaluation of a 68Ga-labeled biotin analogue for applications in islet transplantation.
Topics: Animals; Avidin; Binding, Competitive; Biotin; Gallium Radioisotopes; Humans; Injections, Intravenous; Islets of Langerhans; Islets of Langerhans Transplantation; Male; Mice; Mice, Inbred C57BL; Molecular Structure; Organometallic Compounds; Positron-Emission Tomography; Sepharose; Tissue Distribution | 2012 |
On-bead expression of recombinant proteins in an agarose gel matrix coated on a glass slide.
Topics: Biotin; Cell-Free System; DNA; Glass; Green Fluorescent Proteins; Luciferases; Microscopy, Confocal; Microspheres; Polymerase Chain Reaction; Recombinant Proteins; Sepharose | 2012 |
Alkanethiol-functionalized terahertz metamaterial as label-free, highly-sensitive and specific biosensor.
Topics: Alkanes; Bacterial Proteins; Biosensing Techniques; Biotin; Refractometry; Sepharose; Stearic Acids; Sulfhydryl Compounds; Surface Plasmon Resonance; Terahertz Radiation | 2013 |
Interference from anti-streptavidin antibody.
Topics: Antibodies, Heterophile; Biotin; Diagnostic Errors; Humans; Hyperthyroidism; Immunoassay; Immunosorbent Techniques; Male; Middle Aged; Sepharose; Streptavidin; Thyrotropin; Thyroxine | 2013 |
Analysis of the cell surface expression of cytokine receptors using the surface protein biotinylation method.
Topics: Animals; Bacterial Proteins; Biotin; Biotinylation; Cell Line; Cell Membrane; Gene Expression; Humans; Protein Binding; Receptors, Cytokine; Sepharose; Staining and Labeling; Succinimides | 2014 |
Cell surface biotinylation of receptor tyrosine kinases to investigate intracellular trafficking.
Topics: Biotin; Biotinylation; Blotting, Western; Cell Membrane; Cytosol; Electrophoresis, Polyacrylamide Gel; HEK293 Cells; HeLa Cells; Humans; Protein Transport; Receptor Protein-Tyrosine Kinases; Sepharose; Staining and Labeling; Streptavidin; Succinimides | 2015 |
Purification of CD47-streptavidin fusion protein from bacterial lysate using biotin-agarose affinity chromatography.
Topics: Animals; Biotin; CD47 Antigen; Chromatography, Affinity; Escherichia coli; Mice; Recombinant Fusion Proteins; Sepharose; Streptavidin | 2016 |
Competitive Affinity Release for Long-Term Delivery of Antibodies from Hydrogels.
Topics: Antibodies; Biotin; Delayed-Action Preparations; Drug Carriers; Humans; Hydrogels; Sepharose; Streptavidin | 2018 |
Hydrogels with reversible chemical environments for in vitro cell culture.
Topics: Alginates; Biotin; Cell Culture Techniques; Cell Differentiation; Cell Movement; Cell Proliferation; Culture Media; Fluorescent Dyes; Human Umbilical Vein Endothelial Cells; Humans; Hydrogels; Oligopeptides; Peptides; Sepharose; Streptavidin | 2018 |
Naked eye detection of an amplified gene using metal particle-based DNA transport within functionalized porous interfaces.
Topics: Animals; Avidin; Biotin; Chromatography; Colorimetry; DNA, Bacterial; Escherichia coli O157; Food Contamination; Food Microbiology; Genes, Bacterial; Metals, Heavy; Milk; Polymerase Chain Reaction; Porosity; Sepharose | 2019 |
Variability in Streptavidin-Sepharose Matrix Quality Can Significantly Affect Proximity-Dependent Biotinylation (BioID) Data.
Topics: Biotin; Biotinylation; Peptides; Sepharose; Streptavidin | 2020 |
Protein-responsive protein release of supramolecular/polymer hydrogel composite integrating enzyme activation systems.
Topics: Animals; Antibodies; Avidin; Benzenesulfonamides; Biotin; Carbonic Anhydrase II; Carbonic Anhydrase Inhibitors; Cattle; Delayed-Action Preparations; Enzyme Activation; Hydrogels; Phase Transition; Rheology; Ribonucleases; Sepharose; Sulfonamides | 2020 |