Target type: molecularfunction
An activity that faciliates the formation of a complementary double-stranded DNA molecule. [PMID:22888405, PMID:25520186]
DNA/DNA annealing activity refers to the process by which two single-stranded DNA molecules with complementary base sequences come together to form a double-stranded DNA molecule. This process is essential for many cellular functions, including DNA replication, repair, and recombination. The annealing process is driven by the formation of hydrogen bonds between complementary base pairs. Adenine (A) pairs with thymine (T), and guanine (G) pairs with cytosine (C). These hydrogen bonds are weak individually but collectively contribute to the stability of the double helix. The process of annealing involves several steps: 1. **Strand Alignment:** Complementary strands of DNA must come into close proximity. This can be facilitated by proteins or by random collisions in solution. 2. **Base Pairing:** Complementary bases on the two strands recognize each other and form hydrogen bonds. 3. **Strand Association:** The paired bases begin to associate and form short stretches of double-stranded DNA. 4. **Nucleation:** The short stretches of double-stranded DNA grow, and the two strands become fully associated. 5. **Stability:** The double-stranded DNA molecule is stabilized by the hydrogen bonds between base pairs and by stacking interactions between the bases. The stability of the double-stranded DNA molecule is influenced by several factors, including the length of the DNA strands, the sequence of the DNA, and the temperature and salt concentration of the solution. DNA annealing is a highly regulated process that is influenced by many factors. These factors include the presence of specific proteins that can facilitate or inhibit annealing, the temperature of the solution, and the concentration of ions in the solution. DNA/DNA annealing activity is essential for life and plays a critical role in many cellular processes.'
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| Protein | Definition | Taxonomy |
|---|---|---|
| Bloom syndrome protein | A RecQ-like DNA helicase BLM that is encoded in the genome of human. [PRO:DNx, UniProtKB:P54132] | Homo sapiens (human) |
| ATP-dependent DNA helicase Q1 | An ATP-dependent DNA helicase Q1 that is encoded in the genome of human. [PRO:DNx, UniProtKB:P46063] | Homo sapiens (human) |
| Compound | Definition | Classes | Roles |
|---|---|---|---|
| 1-(3,4-dichlorophenyl)-3-(5-pyridin-4-yl-1,3,4-thiadiazol-2-yl)urea | ureas | ||
| 1-[4-fluoro-3-(trifluoromethyl)phenyl]-3-(5-pyridin-4-yl-1,3,4-thiadiazol-2-yl)urea | ureas |