3' overhang single-stranded DNA endodeoxyribonuclease activity

Definition

Target type: molecularfunction

Catalysis of the hydrolysis of ester linkages within 3' overhang single-stranded deoxyribonucleic acid by creating internal breaks. [PMID:25203555]

3\\' overhang single-stranded DNA endodeoxyribonuclease activity refers to the enzymatic activity that specifically cleaves the phosphodiester bonds within a single-stranded DNA molecule, where the break occurs at the 3\\' end of the molecule. This activity is often associated with enzymes like exonucleases, which degrade DNA from the ends. The 3\\' overhang refers to a single-stranded region extending beyond the double-stranded portion of the DNA molecule. These enzymes play crucial roles in various cellular processes, including DNA repair, replication, and recombination. In DNA repair, they remove damaged or mismatched nucleotides from the 3\\' end of a single-stranded DNA molecule, ensuring the integrity of the genetic code. In replication, they can remove primers, short sequences of RNA or DNA that initiate DNA synthesis. During recombination, these enzymes participate in the processing of DNA ends, facilitating the exchange of genetic information between homologous chromosomes. The specificity of this activity for single-stranded DNA with a 3\\' overhang ensures that the enzyme acts only on the appropriate substrate, preventing unwanted cleavage of double-stranded DNA or other nucleic acid molecules.'
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Proteins (2)

Compounds (2)