NEDD8 ligase activity

Definition

Target type: molecularfunction

Catalysis of the transfer of NEDD8 to a substrate protein via the reaction X-NEDD8 + S = X + S-NEDD8, where X is either an E2 or E3 enzyme, the X-NEDD8 linkage is a thioester bond, and the S-NEDD8 linkage is an isopeptide bond between the C-terminal amino acid of NEDD8 and the epsilon-amino group of lysine residues in the substrate. [GOC:dph]

NEDD8 ligase activity is a critical molecular function involved in the post-translational modification of target proteins with the ubiquitin-like protein NEDD8. This process, known as NEDDylation, is essential for regulating a wide array of cellular processes, including signal transduction, DNA replication, cell cycle progression, and protein degradation. NEDD8 ligases, also known as E3 ligases, specifically recognize and bind to target proteins, facilitating the transfer of NEDD8 from a carrier protein (E2) to a lysine residue on the target protein. The NEDD8 ligase complex typically consists of a catalytic subunit, which possesses the enzymatic activity for NEDD8 conjugation, and a substrate recognition subunit, which determines the specificity of the ligase for particular target proteins. NEDDylation often modulates the activity and stability of target proteins, influencing their interactions with other proteins and their localization within the cell. Disruption of NEDDylation can have significant consequences for cellular function and can contribute to the development of various diseases, including cancer, neurodegenerative disorders, and immune dysfunction. The molecular function of NEDD8 ligase activity plays a crucial role in maintaining cellular homeostasis and ensuring proper regulation of key cellular processes.'
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Proteins (1)

Compounds (19)