Target type: molecularfunction
Catalysis of the reaction: diolein + mono-olein = triolein + glycerol. Mono-olein, also known as mono-oleoylglycerol, is the monoglyceride formed from oleic acid, 9-octodecenoic acid; diolein is also known as dioleoylglycerol, and triolein as trioleoylglycerol and olein. [GOC:ai, PMID:15364929]
Diolein transacylation activity refers to the enzymatic transfer of fatty acyl groups from one molecule of diolein to another. Diolein is a diglyceride composed of two oleic acid molecules linked to a glycerol backbone. This activity is critical for the metabolism of fats and the modification of lipid structures.
The specific mechanism of diolein transacylation involves the following steps:
1. **Substrate binding:** The enzyme binds to a molecule of diolein, positioning it in the active site.
2. **Acyl group transfer:** The enzyme catalyzes the transfer of an acyl group from one diolein molecule to another. This transfer typically occurs at the sn-1 position of the glycerol backbone.
3. **Product release:** The modified diolein molecule and the remaining acyl group are released from the enzyme.
The resulting products of diolein transacylation can vary depending on the specific enzyme involved and the substrate. For example, one common product is triolein, a triglyceride containing three oleic acid molecules.
Diolein transacylation plays a crucial role in various biological processes, including:
* **Lipid synthesis:** It contributes to the synthesis of triacylglycerols, which are essential energy storage molecules.
* **Lipid remodeling:** It modifies the fatty acid composition of lipids, influencing their physical properties and biological functions.
* **Signal transduction:** It may be involved in the regulation of cellular signaling pathways.
In summary, diolein transacylation activity is a fundamental enzymatic process that enables the transfer of fatty acyl groups between diolein molecules, impacting lipid metabolism, structure, and function.'
"
| Protein | Definition | Taxonomy |
|---|---|---|
| Patatin-like phospholipase domain-containing protein 2 | A patatin-like phospholipase domain-containing protein 2 that is encoded in the genome of human. [PRO:DNx, UniProtKB:Q96AD5] | Homo sapiens (human) |
| Compound | Definition | Classes | Roles |
|---|---|---|---|
| atglistatin | atglistatin : A biphenyl that is 1,1'-biphenyl substituted by (dimethylcarbamoyl)amino and dimethylamino groups at positions 3 and 4', respectively. It is a potent inhibitor of adipose triglyceride lipase activity (IC50 = 700nM). atglistatin: inhibits adipose triglyceride lipase; structure in first source |