6-phosphogluconolactonase activity

Definition

Target type: molecularfunction

Catalysis of the reaction: 6-O-phosphono-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate + H+. [EC:3.1.1.31, RHEA:12556]

6-Phosphogluconolactonase (6PGL) is a crucial enzyme in the pentose phosphate pathway (PPP), a metabolic route that generates NADPH and pentose sugars. Its primary role is to catalyze the hydrolysis of 6-phosphogluconolactone (6PGL) to 6-phosphogluconate (6PG). This reaction is a crucial step in the oxidative branch of the PPP. 6PGL is formed by the oxidation of glucose-6-phosphate by glucose-6-phosphate dehydrogenase. The hydrolysis of 6PGL by 6PGLase is a highly specific reaction, and the enzyme exhibits a high affinity for its substrate. The enzyme is also highly regulated, with its activity being influenced by factors such as the concentration of its substrate and the presence of inhibitors. The catalytic mechanism of 6PGLase involves the formation of a covalent intermediate between the enzyme and the substrate. This intermediate is then hydrolyzed to release 6PG and regenerate the free enzyme. The precise mechanism of this reaction has been studied extensively, and it is now well understood. The importance of 6PGLase in the PPP is highlighted by the fact that mutations in the gene encoding this enzyme can lead to a variety of diseases, including hemolytic anemia and neurodevelopmental disorders. In addition to its role in the PPP, 6PGLase has also been implicated in other metabolic pathways. For example, it has been shown to be involved in the biosynthesis of certain vitamins and amino acids. 6PGLase is a highly conserved enzyme, and its structure and function have been studied in detail. The enzyme is typically a monomeric protein, with a molecular weight of around 30 kDa. The active site of the enzyme is located in a deep cleft, which is lined by a variety of amino acid residues that are important for catalysis. The study of 6PGLase has provided important insights into the mechanisms of enzyme catalysis and metabolic regulation. It is a crucial enzyme in the PPP, and its deficiency can have significant health consequences. The detailed understanding of its molecular function has paved the way for the development of new therapeutic strategies for diseases related to its dysfunction.'
"

Proteins (1)

Compounds (1)