Target type: molecularfunction
Catalysis of the reaction: 4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + CMP. [EC:4.6.1.12, RHEA:23864]
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (MCS) catalyzes the formation of 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MEcPP) from 4-diphosphocytidyl-2-C-methyl-D-erythritol (CDP-ME). This reaction is a key step in the non-mevalonate pathway, which is an alternative pathway for the biosynthesis of isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP), essential building blocks for the synthesis of terpenes, steroids, and other isoprenoids. The enzyme utilizes a two-step mechanism. First, MCS binds CDP-ME and Mg2+ ions, which are required for catalysis. Then, MCS catalyzes the intramolecular cyclization of CDP-ME, resulting in the formation of a cyclic phosphate ring. This is followed by the release of CMP (cytidine monophosphate), leading to the formation of MEcPP. This enzyme plays a critical role in the biosynthesis of isoprenoids, which are essential molecules in various biological processes, including photosynthesis, respiration, and hormone synthesis. MCS is a potential target for the development of new antimicrobial agents, as its inhibition can disrupt the synthesis of essential isoprenoids in bacterial pathogens.'
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| Protein | Definition | Taxonomy |
|---|---|---|
| 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase | A 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase that is encoded in the genome of Escherichia coli K-12. [PRO:DNx, UniProtKB:P62617] | Escherichia coli K-12 |
| Compound | Definition | Classes | Roles |
|---|---|---|---|
| cytidine diphosphate | Cytidine Diphosphate: Cytidine 5'-(trihydrogen diphosphate). A cytosine nucleotide containing two phosphate groups esterified to the sugar moiety. Synonyms: CRPP; cytidine pyrophosphate. | cytidine 5'-phosphate; pyrimidine ribonucleoside 5'-diphosphate | Escherichia coli metabolite; mouse metabolite |
| 5-fluorocytidine | 5-fluorocytidine: isolated from DNA of Escherichia coli grown in presence of 5-fluorouracil; structure; RN given refers to parent cpd | cytidines; organofluorine compound | |
| cyclic cmp | 3',5'-cyclic CMP : A 3',5'-cyclic pyrimidine nucleotide having cytosine as the nucleobase. Cyclic CMP: A cyclic nucleotide formed from CYTIDINE TRIPHOSPHATE by the action of cytidylate cyclase. It is a potential cyclic nucleotide intracellular mediator of signal transductions. | 3',5'-cyclic pyrimidine nucleotide | human metabolite |
| cidofovir anhydrous | cidofovir anhydrous : Cytosine substituted at the 1 position by a 3-hydroxy-2-(phosphonomethoxy)propyl group (S configuration). A nucleoside analogue, it is an injectable antiviral used for the treatment of cytomegalovirus (CMV) retinitis in AIDS patients. Cidofovir: An acyclic nucleoside phosphonate that acts as a competitive inhibitor of viral DNA polymerases. It is used in the treatment of RETINITIS caused by CYTOMEGALOVIRUS INFECTIONS and may also be useful for treating HERPESVIRUS INFECTIONS. | phosphonic acids; pyrimidone | anti-HIV agent; antineoplastic agent; antiviral drug; photosensitizing agent |
| 1-beta-d-arabinofuranosylcytosine 5'-monophosphate | 1-beta-D-arabinofuranosylcytosine 5'-monophosphate: RN given refers to dihydrogen phosphate |