Page last updated: 2024-10-24

2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity

Definition

Target type: molecularfunction

Catalysis of the reaction: 6-phospho-2-dehydro-3-deoxy-D-galactonate = D-glyceraldehyde 3-phosphate + pyruvate. [EC:4.1.2.21, RHEA:24464]

2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity catalyzes the reversible aldol cleavage of 2-dehydro-3-deoxy-6-phosphogalactonate to pyruvate and D-glyceraldehyde 3-phosphate. This enzyme is involved in the Entner-Doudoroff pathway, an alternative pathway for glucose metabolism in some bacteria. The enzyme is a member of the class I aldolase family, which is characterized by the use of a Schiff base intermediate in the catalytic mechanism. The active site of the enzyme contains a lysine residue that forms a Schiff base with the substrate. This Schiff base intermediate is then cleaved by a water molecule, resulting in the formation of pyruvate and D-glyceraldehyde 3-phosphate. The enzyme is highly specific for its substrate, 2-dehydro-3-deoxy-6-phosphogalactonate, and does not exhibit activity with other aldolases. 2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity is essential for the growth of bacteria that utilize the Entner-Doudoroff pathway. This enzyme is also a potential target for the development of new antibacterial drugs.'
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Proteins (1)

ProteinDefinitionTaxonomy
2-dehydro-3-deoxy-6-phosphogalactonate aldolaseA 2-dehydro-3-deoxy-6-phosphogalactonate aldolase that is encoded in the genome of Escherichia coli K-12. [PRO:DNx, UniProtKB:Q6BF16]Escherichia coli K-12

Compounds (1)

CompoundDefinitionClassesRoles
2-keto-3-deoxy-6-phosphogluconate2-dehydro-3-deoxy-6-phospho-D-gluconic acid : The 5-phospho derivative of 2-dehydro-D-gluconic acid.

2-keto-3-deoxy-6-phosphogluconate: structure given in first source; an intermediate in glucose metabolism
ketoaldonic acid phosphateEscherichia coli metabolite