dCMP deaminase activity
Definition
Target type: molecularfunction
Catalysis of the reaction: dCMP + H2O = dUMP + NH3. [EC:3.5.4.12]
dCMP deaminase activity catalyzes the hydrolytic deamination of deoxycytidine 5'-monophosphate (dCMP) to deoxythymidine 5'-monophosphate (dTMP). This reaction is crucial for the biosynthesis of deoxythymidine triphosphate (dTTP), a key component of DNA. dCMP deaminase activity is highly specific for dCMP, exhibiting negligible activity towards other deoxynucleotides or ribonucleotides. The reaction mechanism involves the abstraction of a proton from the C-5 position of dCMP by an active site residue, followed by the formation of a covalent intermediate between the enzyme and the substrate. This intermediate is then hydrolyzed, releasing dTMP and regenerating the enzyme. dCMP deaminase activity is tightly regulated, ensuring that dTTP synthesis is balanced with the synthesis of other deoxynucleotides to maintain the integrity of DNA replication.'
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Proteins (1)
| Protein | Definition | Taxonomy |
|---|---|---|
| Deoxycytidylate deaminase | A deoxycytidylate deaminase that is encoded in the genome of human. [PRO:DNx, UniProtKB:P32321] | Homo sapiens (human) |
Compounds (1)
| Compound | Definition | Classes | Roles |
|---|---|---|---|
| deoxycytidine monophosphate | 2'-deoxycytosine 5'-monophosphate : A pyrimidine 2'-deoxyribonucleoside 5'-monophosphate having cytosine as the nucleobase. Deoxycytidine Monophosphate: Deoxycytidine (dihydrogen phosphate). A deoxycytosine nucleotide containing one phosphate group esterified to the deoxyribose moiety in the 2'-,3'- or 5- positions. | 2'-deoxycytidine phosphate; pyrimidine 2'-deoxyribonucleoside 5'-monophosphate | Escherichia coli metabolite; mouse metabolite |