Target type: molecularfunction
Catalysis of the reaction: beta-D-fructose 6-phosphate + ATP = beta-D-fructose 2,6-bisphosphate + ADP + 2 H+. [EC:2.7.1.105, RHEA:15653]
6-phosphofructo-2-kinase (PFK-2) activity is a key regulatory step in glycolysis and gluconeogenesis. It catalyzes the phosphorylation of fructose 6-phosphate to fructose 2,6-bisphosphate (F2,6BP). This reaction is crucial for the regulation of carbohydrate metabolism as F2,6BP acts as a potent allosteric activator of phosphofructokinase 1 (PFK-1), the rate-limiting enzyme of glycolysis, and an inhibitor of fructose 1,6-bisphosphatase (FBPase-1), the rate-limiting enzyme of gluconeogenesis. In essence, PFK-2 activity promotes glycolysis by enhancing PFK-1 activity and suppressing FBPase-1 activity, leading to increased glucose utilization and ATP production. Conversely, reduced PFK-2 activity favors gluconeogenesis by decreasing PFK-1 activity and enhancing FBPase-1 activity, allowing the synthesis of glucose from non-carbohydrate precursors.'
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| Protein | Definition | Taxonomy |
|---|---|---|
| 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 | A 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 that is encoded in the genome of human. [PRO:DNx, UniProtKB:Q16875] | Homo sapiens (human) |
| Compound | Definition | Classes | Roles |
|---|---|---|---|
| 2-(4-hydroxyphenyl)-5,6,7,8-tetrahydroxy-4H-1-benzopyran-4-one | 2-(4-hydroxyphenyl)-5,6,7,8-tetrahydroxy-4H-1-benzopyran-4-one : A pentahydroxyflavone that is flavone substituted by hydroxy groups at positions 5, 6, 7, 8, and 4' respectively. | pentahydroxyflavone | |
| 4',7,8-trihydroxyisoflavone | 4',7,8-trihydroxyisoflavone: from Streptomyces sp OH-1049; structure given in first source | isoflavones | |
| 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one | 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one: 6-phosphofructo-2-kinase-fructose-2,6-bisphosphatase isozymes inhibitor; structure in first source | enone; pyridines | angiogenesis inhibitor; antineoplastic agent; apoptosis inducer; autophagy inducer; EC 2.7.1.105 (6-phosphofructo-2-kinase) inhibitor |