aminocarboxymuconate-semialdehyde decarboxylase activity
Definition
Target type: molecularfunction
Catalysis of the reaction: 2-amino-3-carboxymuconate 6-semialdehyde + H+ = 2-aminomuconate 6-semialdehyde + CO2. [EC:4.1.1.45, RHEA:16557]
Aminocarboxymuconate-semialdehyde decarboxylase activity is a crucial step in the degradation pathway of aromatic compounds, particularly in the metabolism of the amino acid L-lysine and the aromatic compound benzoate. This enzymatic activity is catalyzed by the enzyme aminocarboxymuconate-semialdehyde decarboxylase (ACMSD), which is a pyridoxal phosphate-dependent decarboxylase.
The molecular function of ACMSD involves the removal of a carboxyl group (COO-) from aminocarboxymuconate-semialdehyde, a key intermediate in the pathway. This decarboxylation reaction is crucial for generating a molecule called 2-aminomuconate-semialdehyde, a crucial precursor in the further degradation of the aromatic compound.
The detailed molecular function of ACMSD can be summarized as follows:
1. **Substrate Binding:** ACMSD binds to the substrate, aminocarboxymuconate-semialdehyde, through interactions with its active site.
2. **Pyridoxal Phosphate (PLP) Cofactor:** The enzyme utilizes pyridoxal phosphate (PLP) as a cofactor. PLP forms a Schiff base with the ε-amino group of a lysine residue in the active site, creating a highly reactive intermediate.
3. **Decarboxylation:** The bound substrate undergoes a decarboxylation reaction, where the carboxyl group is removed and released as carbon dioxide (CO2). This reaction is facilitated by the PLP cofactor, which acts as a catalytic agent.
4. **Product Release:** The decarboxylated product, 2-aminomuconate-semialdehyde, is released from the enzyme, completing the catalytic cycle.
In summary, aminocarboxymuconate-semialdehyde decarboxylase activity is essential for the degradation of aromatic compounds. The enzyme catalyzes the removal of a carboxyl group from aminocarboxymuconate-semialdehyde, generating a precursor for further degradation in the pathway. This activity is crucial for maintaining cellular homeostasis and the proper functioning of metabolic processes.'
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Proteins (1)
| Protein | Definition | Taxonomy |
|---|---|---|
| 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase | A 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase that is encoded in the genome of human. [PRO:DNx, UniProtKB:Q8TDX5] | Homo sapiens (human) |
Compounds (2)
| Compound | Definition | Classes | Roles |
|---|---|---|---|
| diflunisal | diflunisal : An organofluorine compound comprising salicylic acid having a 2,4-difluorophenyl group at the 5-position. Diflunisal: A salicylate derivative and anti-inflammatory analgesic with actions and side effects similar to those of ASPIRIN. | monohydroxybenzoic acid; organofluorine compound | non-narcotic analgesic; non-steroidal anti-inflammatory drug |
| dipicolinic acid | dipicolinic acid : A pyridinedicarboxylic acid carrying two carboxy groups at positions 2 and 6. | pyridinedicarboxylic acid | bacterial metabolite |