Target type: biologicalprocess
The incorporation of two iron atoms and two sulfur atoms into an iron-sulfur cluster. [GOC:jl, GOC:mengo_curators, GOC:pde, GOC:tt, GOC:vw, PMID:15952888]
[2Fe-2S] cluster assembly is a complex process that involves multiple proteins and steps. It begins with the synthesis of the iron-sulfur (Fe-S) cluster precursor, which is a simple inorganic complex of iron and sulfide ions. This precursor is then assembled into the final [2Fe-2S] cluster, which is a more complex structure that contains two iron atoms and two sulfur atoms. The assembly process takes place in a protein scaffold known as the scaffold protein.
The scaffold protein binds to the Fe-S cluster precursor and then uses a series of enzymatic reactions to assemble the final [2Fe-2S] cluster. These reactions involve the transfer of electrons and protons, as well as the formation of new chemical bonds. Once the [2Fe-2S] cluster is assembled, it is transferred to its target protein.
The target protein is typically an enzyme that requires a [2Fe-2S] cluster for its function. The cluster is incorporated into the protein's active site, where it plays a role in the enzyme's catalytic activity.
In summary, the biological process of [2Fe-2S] cluster assembly can be broken down into the following steps:
1. **Synthesis of the Fe-S cluster precursor** This involves the combination of iron and sulfide ions.
2. **Assembly of the [2Fe-2S] cluster** This occurs within a protein scaffold known as the scaffold protein.
3. **Transfer of the [2Fe-2S] cluster to its target protein** This involves the incorporation of the cluster into the target protein's active site.
[2Fe-2S] cluster assembly is a vital process for many cellular functions. These clusters are required for a variety of enzymatic reactions, including electron transport, DNA replication, and the metabolism of iron and sulfur. Defects in [2Fe-2S] cluster assembly can lead to a number of human diseases, including Friedreich's ataxia and mitochondrial disorders.'
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| Protein | Definition | Taxonomy |
|---|---|---|
| Acyl carrier protein, mitochondrial | An acyl carrier protein, mitochondrial that is encoded in the genome of cow. [OMA:P52505, PRO:DNx] | Bos taurus (cattle) |
| Acyl carrier protein, mitochondrial | An acyl carrier protein, mitochondrial that is encoded in the genome of human. [PRO:DNx, UniProtKB:O14561] | Homo sapiens (human) |
| Compound | Definition | Classes | Roles |
|---|---|---|---|
| rotenone | Derris: A plant genus of the family FABACEAE. The root is a source of rotenoids (ROTENONE) and flavonoids. Some species of Pongamia have been reclassified to this genus and some to MILLETTIA. Some species of Deguelia have been reclassified to this genus. rotenoid : Members of the class of tetrahydrochromenochromene that consists of a cis-fused tetrahydrochromeno[3,4-b]chromene skeleton and its substituted derivatives. The term was originally restricted to natural products, but is now also used to describe semi-synthetic and fully synthetic compounds. | organic heteropentacyclic compound; rotenones | antineoplastic agent; metabolite; mitochondrial NADH:ubiquinone reductase inhibitor; phytogenic insecticide; piscicide; toxin |
| desacetyluvaricin | desacetyluvaricin: isolated from Uvaria accuminata; structure given in first source | ||
| squamocin | squamocin: from seeds of Annona squamosa; a trihydroxy-bis-tetrahydrofuran fatty acid lactone; inhibits E. coli NADH:quinone oxidoreductase (NDH-1); structure given in first source | polyketide | |
| aurapten | aurapten: RN refers to (E)-isomer; structure given in first source auraptene : A member of the class of coumarins that is umbelliferone in which the phenolic hydrogen has been replaced by a geranyl group. Ii is isolated from several edible fruits and vegetables and exhibits a variety of therapeutic properties. | coumarins; monoterpenoid | antihypertensive agent; antineoplastic agent; antioxidant; apoptosis inducer; dopaminergic agent; EC 2.7.11.24 (mitogen-activated protein kinase) inhibitor; gamma-secretase modulator; gastrointestinal drug; hepatoprotective agent; matrix metalloproteinase inhibitor; neuroprotective agent; plant metabolite; PPARalpha agonist; vulnerary |
| piericidin a | piericidin A : A member of the class of monohydroxypyridines that acts as an irreversible mitochondrial Complex I inhibitor that strongly associates with ubiquinone binding sites in both mitochondrial and bacterial forms of NADH:ubiquinone oxidoreductase piericidin A: pyridine-substituted fatty alcohol antibiotic; minor descriptor (75-85); on-line & Index Medicus search ANTIBIOTICS (75-85); RN given refers to (S-(R*,R*-(all-E)))-isomer | aromatic ether; methylpyridines; monohydroxypyridine; secondary allylic alcohol | antimicrobial agent; bacterial metabolite; EC 1.6.5.3 [NADH:ubiquinone reductase (H(+)-translocating)] inhibitor; mitochondrial respiratory-chain inhibitor |
| marmin | marmin: structure in first source; RN given for (R-(E))-isomer | ||
| laherradurin | laherradurin: structure in first source |