maturation of LSU-rRNA

Definition

Target type: biologicalprocess

Any process involved in the maturation of a precursor Large SubUnit (LSU) ribosomal RNA (rRNA) molecule into a mature LSU-rRNA molecule. [GOC:curators]

The maturation of LSU-rRNA, also known as the large subunit rRNA, is a complex and highly regulated process that involves a series of enzymatic modifications and cleavages. It begins with the transcription of a large precursor rRNA molecule, known as pre-rRNA, from the ribosomal DNA (rDNA) gene. This precursor contains the sequences for both the LSU-rRNA and the small subunit rRNA (SSU-rRNA).

The first step in LSU-rRNA maturation is the processing of the 5' end of the pre-rRNA. This involves the removal of a 5' external transcribed spacer (5' ETS) by an exonuclease. Subsequently, a series of endonucleolytic cleavages by ribonucleases (RNases) occur, leading to the formation of the 5.8S rRNA and a large fragment containing the LSU-rRNA.

Following the initial cleavage events, the LSU-rRNA undergoes a series of modifications, including methylation and pseudouridylation. These modifications are crucial for the proper folding and function of the mature rRNA. They are catalyzed by specific enzymes that recognize and modify specific nucleotides within the rRNA sequence.

Concomitantly with these modifications, the LSU-rRNA undergoes further processing steps, including the removal of internal transcribed spacers (ITS) and the formation of the mature 25S rRNA in eukaryotes or 23S rRNA in prokaryotes. These processing steps involve additional endonucleolytic cleavages and exonucleolytic trimming by specific RNases.

The final step in LSU-rRNA maturation involves the association of the mature LSU-rRNA with ribosomal proteins to form the large ribosomal subunit. This assembly process is tightly regulated and involves the coordinated action of chaperone proteins and other factors.

Throughout the entire maturation process, the LSU-rRNA undergoes a series of conformational changes, guided by the modifications and interactions with ribosomal proteins. These changes ensure that the rRNA adopts the correct three-dimensional structure essential for its function in protein synthesis.

The maturation of LSU-rRNA is a critical step in ribosome biogenesis and is essential for the production of all proteins in the cell. Any defects in this process can lead to severe cellular dysfunction and disease.'
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Proteins (1)

Compounds (2)