Methylated-DNA--protein-cysteine methyltransferase

A methylated-DNA--protein-cysteine methyltransferase that is encoded in the genome of human. [PRO:DNx, UniProtKB:P16455]

Synonyms

EC 2.1.1.63;
6-O-methylguanine-DNA methyltransferase;
MGMT;
O-6-methylguanine-DNA-alkyltransferase

Research

Bioassay Publications (10)

Timeframe	Studies on this Protein(%)	All Drugs %
pre-1990	0 (0.00)	18.7374
1990's	4 (40.00)	18.2507
2000's	4 (40.00)	29.6817
2010's	2 (20.00)	24.3611
2020's	0 (0.00)	2.80

Compounds (8)

Drugs with Inhibition Measurements

Drug	Taxonomy	Measurement	Average (mM)	Bioassay(s)	Publication(s)
nu2058	Homo sapiens (human)	IC50	1,000.0000	1	1
o(6)-benzylguanine	Homo sapiens (human)	IC50	0.5196	7	7
o-(6)-methylguanine	Homo sapiens (human)	IC50	338.0000	2	2
o(6)-n-butylguanine	Homo sapiens (human)	IC50	493.0000	1	1
6-ethylguanine	Homo sapiens (human)	IC50	1,000.0000	1	1
2,4-diamino-6-benzyloxy-5-nitrosopyrimidine	Homo sapiens (human)	IC50	0.1300	1	1
lomeguatrib	Homo sapiens (human)	IC50	0.0060	2	2

Drugs with Other Measurements

Drug	Taxonomy	Measurement	Average (mM)	Bioassay(s)	Publication(s)
o(6)-benzylguanine	Homo sapiens (human)	ED50	168.1723	13	13
o-(6)-methylguanine	Homo sapiens (human)	ED50	235.0000	2	2
o(6)-benzyl-2'-deoxyguanosine	Homo sapiens (human)	ED50	1.2500	2	2
2,4-diamino-6-benzyloxy-5-nitrosopyrimidine	Homo sapiens (human)	ED50	0.0400	2	2

Enables

This protein enables 5 target(s):

Target	Category	Definition
DNA binding	molecular function	Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid). [GOC:dph, GOC:jl, GOC:tb, GOC:vw]
methyltransferase activity	molecular function	Catalysis of the transfer of a methyl group to an acceptor molecule. [ISBN:0198506732]
DNA-methyltransferase activity	molecular function	Catalysis of the transfer of a methyl group to a DNA molecule. [GOC:jl, ISBN:0198506732, PMID:7862522]
metal ion binding	molecular function	Binding to a metal ion. [GOC:ai]
methylated-DNA-[protein]-cysteine S-methyltransferase activity	molecular function	Catalysis of the reaction: DNA (containing 6-O-methylguanine) + (protein)-L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine. [EC:2.1.1.63]

Located In

This protein is located in 2 target(s):

Target	Category	Definition
nucleoplasm	cellular component	That part of the nuclear content other than the chromosomes or the nucleolus. [GOC:ma, ISBN:0124325653]
membrane	cellular component	A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. [GOC:dos, GOC:mah, ISBN:0815316194]

Active In

This protein is active in 1 target(s):

Target	Category	Definition
nucleus	cellular component	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. [GOC:go_curators]

Involved In

This protein is involved in 6 target(s):

Target	Category	Definition
DNA ligation	biological process	The re-formation of a broken phosphodiester bond in the DNA backbone, carried out by DNA ligase. [ISBN:0815316194]
DNA alkylation repair	biological process	The repair of alkylation damage in DNA, e.g. the removal of a non-physiological alkyl group from a nucleobase. This is usually mediated by DNA alkyltransferases. [PMID:10946226, PMID:35543797]
methylation	biological process	The process in which a methyl group is covalently attached to a molecule. [GOC:mah]
negative regulation of apoptotic process	biological process	Any process that stops, prevents, or reduces the frequency, rate or extent of cell death by apoptotic process. [GOC:jl, GOC:mtg_apoptosis]
positive regulation of double-strand break repair	biological process	Any process that activates or increases the frequency, rate or extent of double-strand break repair. [GOC:BHF]
DNA repair	biological process	The process of restoring DNA after damage. Genomes are subject to damage by chemical and physical agents in the environment (e.g. UV and ionizing radiations, chemical mutagens, fungal and bacterial toxins, etc.) and by free radicals or alkylating agents endogenously generated in metabolism. DNA is also damaged because of errors during its replication. A variety of different DNA repair pathways have been reported that include direct reversal, base excision repair, nucleotide excision repair, photoreactivation, bypass, double-strand break repair pathway, and mismatch repair pathway. [PMID:11563486]

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