DNA-directed RNA polymerase, mitochondrial

A DNA-directed RNA polymerase, mitochondrial that is encoded in the genome of human. [PRO:DNx, UniProtKB:O00411]

MtRPOL;
EC 2.7.7.6

Research

Drug	Taxonomy	Measurement	Average (mM)	Bioassay(s)	Publication(s)
GS-443902	Homo sapiens (human)	IC50	200.0000	1	1

This protein enables 7 target(s):

Target	Category	Definition
3'-5'-RNA exonuclease activity	molecular function	Catalysis of the sequential cleavage of mononucleotides from a free 3' terminus of an RNA molecule. [GOC:mah, ISBN:0198547684]
RNA binding	molecular function	Binding to an RNA molecule or a portion thereof. [GOC:jl, GOC:mah]
DNA primase activity	molecular function	Catalysis of the synthesis of a short RNA primer on a DNA template, providing a free 3'-OH that can be extended by DNA-directed DNA polymerases. In certain conditions, for example in response to DNA damage, some primases synthesize a DNA primer. [GOC:mah, GOC:mcc, PMID:11395402, PMID:26184436, PMID:38203225, PMID:38492718]
DNA-directed 5'-3' RNA polymerase activity	molecular function	Catalysis of the reaction: nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). Utilizes a DNA template, i.e. the catalysis of DNA-template-directed extension of the 3'-end of an RNA strand by one nucleotide at a time. Can initiate a chain 'de novo'. [EC:2.7.7.6, GOC:pf]
protein binding	molecular function	Binding to a protein. [GOC:go_curators]
sequence-specific DNA binding	molecular function	Binding to DNA of a specific nucleotide composition, e.g. GC-rich DNA binding, or with a specific sequence motif or type of DNA e.g. promotor binding or rDNA binding. [GOC:jl]
mitochondrial promoter sequence-specific DNA binding	molecular function	Binding to a DNA region that controls the transcription of the mitochondrial DNA. [GOC:txnOH, GOC:vw, PMID:20056105]

This protein is located in 3 target(s):

Target	Category	Definition
mitochondrion	cellular component	A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration. [GOC:giardia, ISBN:0198506732]
mitochondrial matrix	cellular component	The gel-like material, with considerable fine structure, that lies in the matrix space, or lumen, of a mitochondrion. It contains the enzymes of the tricarboxylic acid cycle and, in some organisms, the enzymes concerned with fatty acid oxidation. [GOC:as, ISBN:0198506732]
mitochondrial nucleoid	cellular component	The region of a mitochondrion to which the DNA is confined. [GOC:jl]

This protein is part of 2 target(s):

Target	Category	Definition
protein-containing complex	cellular component	A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together. [GOC:dos, GOC:mah]
mitochondrial DNA-directed RNA polymerase complex	cellular component	A DNA-directed RNA polymerase complex located in the mitochondrion. Mitochondrial RNA polymerase is composed of two subunits, a catalytic core, which resembles the enzymes from bacteriophage T7 and T3, and a specificity factor required for promoter recognition, which is similar to members of the eubacterial sigma factor family. In S. cerevisiae, these are encoded by the nuclear genes RPO41 and MTF1 and the specificity factor, required for promoter recognition and initiation, is not present in the elongating form. [GOC:krc, GOC:mah, PMID:7929382]

This protein is involved in 3 target(s):

Target	Category	Definition
DNA replication, synthesis of primer	biological process	The synthesis of a short nucleotide polymer using one strand of unwound DNA as a template. The product is usually a RNA molecule between 4-15 nucleotides long that provides a free 3'-OH that can be extended by DNA-directed DNA polymerases. In certain conditions, for example in response to DNA damage, some primases synthesize a DNA primer. [PMID:11395402, PMID:38203225, PMID:38492718]
mitochondrial transcription	biological process	The synthesis of RNA from a mitochondrial DNA template, usually by a specific mitochondrial RNA polymerase. [GOC:jl, PMID:23632312]
transcription initiation at mitochondrial promoter	biological process	A transcription initiation process that takes place at a promoter on the mitochondrial chromosome. [PMID:33127643]