withaferin-a and Leukemia-P388

withaferin-a has been researched along with Leukemia-P388* in 4 studies

Other Studies

4 other study(ies) available for withaferin-a and Leukemia-P388

ArticleYear
Microbiological and chemical dehydrogenation of withaferin A.
    Folia microbiologica, 1987, Volume: 32, Issue:2

    Arthrobacter simplex dehydrogenated withaferin A to 4-dehydrowithaferin A but it was not able to dehydrogenate this substrate in position 27. 27-Dehydrowithaferin A was prepared chemically using pyridinium chlorochromate. Whereas 4-dehydrowithaferin A surpassed in its effect on leukemic (388 cells the original compound and all its derivates synthesized so far, 27-dehydrowithaferin A was biologically inactive.

    Topics: Animals; Arthrobacter; Cell Line; Cell Survival; Chemical Phenomena; Chemistry; Ergosterol; Leukemia P388; Leukemia, Experimental; Withanolides

1987
Hydrolysis of withaferin A-4,27-diacetate.
    Die Pharmazie, 1986, Volume: 41, Issue:4

    Topics: Animals; Antineoplastic Agents; Cell Division; Ergosterol; Hydrolysis; Leukemia P388; Magnetic Resonance Spectroscopy; Mice; Withanolides

1986
Novel cytotoxic and antitumor agents. IV. Withaferin A: relation of its structure to the in vitro cytotoxic effects on P388 cells.
    Neoplasma, 1984, Volume: 31, Issue:1

    In vitro effects of withaferin A and its 9 new derivatives on P388 cells have been studied. The cytotoxicity was calculated from the utilization of precursors in protein and nucleic acid (NA) synthesis and from capacity to suppress cell proliferation. The most potent agents proved to be 4-dehydrowithaferin A and withaferin A diacetate exhibited an equal inhibitory effect on thymidine, uridine, and L-valine incorporation. They stopped cell proliferation and, at the same time, killed the cells. Cytotoxicity was found to be due to a double bond at position C2-3, by dissociating this bond the cytotoxicity markedly decreased in all derivatives. A dissociation of the double bond at C24-25 or a removal of OH group from C27 did not cause any significant changes in the biological effects of the derivatives. An addition of a carbonyl group at C4 increased the effects of the agent. An addition of OH groups to the molecule of withaferin A resulted chiefly in a qualitative change in the action of derivatives manifested by a significant decrease in L-valine inhibition. As withaferin A promptly reacted with L-cysteine, it was presumed that one of the possible target sites in the cell might be the SH groups of enzymes which react with the lactone and epoxide groups of the agent.

    Topics: Animals; Antineoplastic Agents, Phytogenic; Cell Division; Cell Line; Cell Survival; Ergosterol; Leukemia P388; Leukemia, Experimental; Mice; Structure-Activity Relationship; Withanolides

1984
Microbial transformations of natural antitumor agents. 23. Conversion of withaferin-A to 12 beta- and 15 beta-hydroxy derivatives of withaferin-A.
    Steroids, 1982, Volume: 40, Issue:2

    Microbial transformation experiments were conducted with the antitumor lactone withaferin-A. Cunninghamella elegans NRRL 1393 transformed withaferin-A (1a) to 15 beta-hydroxywithaferin-A (2a) and 12 beta-hydroxy-withaferin-A (3a). The hydroxylated metabolites were isolated by solvent extraction and were purified by column and thin-layer chromatography. Structures of the hydroxylated metabolites were determined by proton-and carbon-13 NMR, IR and mass spectral analyses, and by the preparation of acylated derivatives. Compounds 2a and 3a inhibited the growth and biochemical functions of in vitro grown P-388 lymphocytic leukemic cells.

    Topics: Animals; Antineoplastic Agents, Phytogenic; Biotransformation; Ergosterol; Fermentation; Leukemia P388; Magnetic Resonance Spectroscopy; Mice; Mucorales; Withanolides

1982
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