naloxone and Fibroma

To study the influence of morphine on lymphokine production from mouse peritoneal macrophages (PM phi).. The productions of interleukin-1 (IL-1) and tumor necrosis factor (TNF-alpha) from PM phi under various concentrations of morphine with or without naloxone were measured by testing thymocyte proliferation and L929 cell lysis in vitro.. Both morphine (1 x 10(-8) - 1 x 10(-3) mol L-1) and naloxone (50 mumol L-1) inhibited IL-1 production from mouse PM phi primed by sodium thioglycolate. Naloxone did not block the inhibitory effect of morphine. TNF-alpha production from PM phi was inhibited by only high concentration (1 mmol L-1) of morphine which was not blocked by naloxone.. Morphine had a direct inhibitory effect on PM phi, which was not mediated by opioid receptor of PM phi.

Topics: Animals; Female; Fibroma; Immunosuppressive Agents; Interleukin-1; Macrophages, Peritoneal; Mice; Mice, Inbred BALB C; Mice, Inbred ICR; Morphine; Naloxone; Narcotic Antagonists; Tumor Cells, Cultured; Tumor Necrosis Factor-alpha