jwh-133 and Pain

jwh-133 has been researched along with Pain* in 4 studies

Other Studies

4 other study(ies) available for jwh-133 and Pain

ArticleYear
Cannabinoid CB2 receptors regulate central sensitization and pain responses associated with osteoarthritis of the knee joint.
    PloS one, 2013, Volume: 8, Issue:11

    Osteoarthritis (OA) of the joint is a prevalent disease accompanied by chronic, debilitating pain. Recent clinical evidence has demonstrated that central sensitization contributes to OA pain. An improved understanding of how OA joint pathology impacts upon the central processing of pain is crucial for the identification of novel analgesic targets/new therapeutic strategies. Inhibitory cannabinoid 2 (CB2) receptors attenuate peripheral immune cell function and modulate central neuro-immune responses in models of neurodegeneration. Systemic administration of the CB2 receptor agonist JWH133 attenuated OA-induced pain behaviour, and the changes in circulating pro- and anti-inflammatory cytokines exhibited in this model. Electrophysiological studies revealed that spinal administration of JWH133 inhibited noxious-evoked responses of spinal neurones in the model of OA pain, but not in control rats, indicating a novel spinal role of this target. We further demonstrate dynamic changes in spinal CB2 receptor mRNA and protein expression in an OA pain model. The expression of CB2 receptor protein by both neurones and microglia in the spinal cord was significantly increased in the model of OA. Hallmarks of central sensitization, significant spinal astrogliosis and increases in activity of metalloproteases MMP-2 and MMP-9 in the spinal cord were evident in the model of OA pain. Systemic administration of JWH133 attenuated these markers of central sensitization, providing a neurobiological basis for analgesic effects of the CB2 receptor in this model of OA pain. Analysis of human spinal cord revealed a negative correlation between spinal cord CB2 receptor mRNA and macroscopic knee chondropathy. These data provide new clinically relevant evidence that joint damage and spinal CB2 receptor expression are correlated combined with converging pre-clinical evidence that activation of CB2 receptors inhibits central sensitization and its contribution to the manifestation of chronic OA pain. These findings suggest that targeting CB2 receptors may have therapeutic potential for treating OA pain.

    Topics: Animals; Cannabinoids; Electrophysiology; Enzyme-Linked Immunosorbent Assay; Humans; Matrix Metalloproteinase 2; Matrix Metalloproteinase 9; Osteoarthritis, Knee; Pain; Rats; Rats, Sprague-Dawley; Receptor, Cannabinoid, CB2; Spinal Cord

2013
Design, synthesis, and pharmacological characterization of indol-3-ylacetamides, indol-3-yloxoacetamides, and indol-3-ylcarboxamides: potent and selective CB2 cannabinoid receptor inverse agonists.
    Journal of medicinal chemistry, 2012, Jun-14, Volume: 55, Issue:11

    In our search for new cannabinoid receptor modulators, we describe herein the design and synthesis of three sets of indole-based ligands characterized by an acetamide, oxalylamide, or carboxamide chain, respectively. Most of the compounds showed affinity for CB2 receptors in the nanomolar range, with K(i) values spanning 3 orders of magnitude (377-0.37 nM), and moderate to good selectivity over CB1 receptors. Their in vitro functional activity as inverse agonists was confirmed in vivo in the formalin test of acute peripheral and inflammatory pain in mice, in which compounds 10a and 11e proved to be able to reverse the effect of the CB2 selective agonist COR167.

    Topics: Amides; Animals; CHO Cells; Combinatorial Chemistry Techniques; Cricetinae; Cricetulus; Cyclic AMP; Drug Design; Drug Inverse Agonism; HEK293 Cells; Humans; Immunologic Factors; Indoles; Inflammation; Mice; Models, Molecular; Pain; Pain Measurement; Radioligand Assay; Receptor, Cannabinoid, CB2; Structure-Activity Relationship

2012
Involvement of central cannabinoid CB2 receptor in reducing mechanical allodynia in a mouse model of neuropathic pain.
    European journal of pharmacology, 2008, Mar-31, Volume: 583, Issue:1

    We sought to examine the involvement of central cannabinoid CB2 receptor activation in modulating mechanical allodynia in a mouse model of neuropathic pain. JWH133 was demonstrated to be a selective cannabinoid CB2 receptor agonist in mice, reducing forskolin-stimulated cAMP production in CHO cells expressing mouse cannabinoid CB2 and cannabinoid CB1 receptors with EC50 values of 63 nM and 2500 nM, respectively. Intrathecal administration of JWH133 (50 and 100 nmol/mouse) significantly reversed partial sciatic nerve ligation-induced mechanical allodynia in mice at 0.5 h after administration. In contrast, systemic (intraperitoneal) or local (injected to the dorsal surface of the hindpaw) administration of JWH133 (100 nmol/mouse) was ineffective. Furthermore, the analgesic effects of intrathecal JWH133 (100 nmol/mouse) were absent in cannabinoid CB2 receptor knockout mice. These results suggest that the activation of central, but not peripheral, cannabinoid CB2 receptors play an important role in reducing mechanical allodynia in a mouse model of neuropathic pain.

    Topics: Animals; Cannabinoids; CHO Cells; Cricetinae; Cricetulus; Cyclic AMP; Female; Genetic Vectors; Indicators and Reagents; Injections, Intraperitoneal; Injections, Spinal; Ligation; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Pain; Peripheral Nervous System Diseases; Physical Stimulation; Receptor, Cannabinoid, CB2; Sciatic Nerve; Sciatic Neuropathy; Transfection

2008
Influence of nicotinic receptor modulators on CB2 cannabinoid receptor agonist (JWH133)-induced antinociception in mice.
    Behavioural pharmacology, 2007, Volume: 18, Issue:7

    Delta9-tetrahydrocannabinol is the active component in cannabis and has long been associated with pain relief. This effect is believed to be mediated through central and peripheral CB1 and peripheral CB2 receptors. We have explored the possible antinociceptive effect of a CB2 receptor agonist, JWH133, using the formalin test in mice. The drug was administered by the intracerebroventricular and intraperitoneal routes. Although no antinociceptive effect was observed after intracerebroventricular administration of JWH133, when the drug was administered by the intraperitoneal route, it produced an analgesic effect. The influence of nicotinic cholinergic receptor modulators, nicotine and mecamylamine, on antinociceptive effect of JWH133 was also studied. Nicotine increased and mecamylamine decreased the antinociceptive effect of JWH133. It is concluded that JWH133-induced analgesia is influenced by nicotinic cholinergic receptor activity.

    Topics: Animals; Cannabinoids; Dose-Response Relationship, Drug; Formaldehyde; Male; Mecamylamine; Mice; Motor Activity; Nicotine; Nicotinic Agonists; Nicotinic Antagonists; Pain; Pain Threshold; Receptor, Cannabinoid, CB2; Receptors, Nicotinic

2007