sepharose has been researched along with puerarin* in 2 studies
2 other study(ies) available for sepharose and puerarin
| Article | Year |
|---|---|
Separation and purification of puerarin using beta-cyclodextrin-coupled agarose gel media.
The isoflavonoid puerarin, a well-known traditional Chinese drug, has been purified in one step from an extract of Radix puerariae (root of the plant Pueralria lobata) by adsorption chromatography on an epichlorohydrin polymerized beta-cyclodextrin ligand coupled to brominated allyl-group substituted Sepharose HP. Acetic acid (10%) was used as the mobile phase and the optimum loading capacity was around 1.2 mg crude extract/ml packed gel. The purity of the collected puerarin was about 98% with a recovery of about 62%. Topics: beta-Cyclodextrins; Chromatography, High Pressure Liquid; Cyclodextrins; Isoflavones; Sepharose | 2004 |
Purification of the isoflavonoid puerarin by adsorption chromatography on cross-linked 12% agarose.
The isoflavonoid puerarin in extracts of the well-known traditional Chinese drug Radix puerariae (root of the plant Pueraria lobata) can be separated from other isoflavonoids by adsorption chromatography on the cross-linked 12% agarose gel Superose 12 equilibrated in distilled water. The adsorption is totally quenched by the addition of 50% acetic acid. The separation of the isoflavonoids is tentatively ascribed to interaction with the residues of the cross-linking reagents used in the manufacturing process of Superose 12. Thus, no useful separation can be achieved with non-cross-linked 12% agarose gel media. Symmetric elution profiles at high sample loadings (16 mg on a 24 ml column) suggest linear adsorption isotherms for the isoflavonoids. Topics: Adsorption; Chromatography, High Pressure Liquid; Isoflavones; Sepharose | 2004 |