sepharose and daidzin

sepharose has been researched along with daidzin* in 1 studies

Other Studies

1 other study(ies) available for sepharose and daidzin

ArticleYear
Preparation of highly pure daidzin on oligo-β-cyclodextrin-Sepharose HP and investigation of chromatographic behavior of isoflavones by molecular docking.
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2011, Jun-15, Volume: 879, Issue:20

    A novel method using column chromatography on oligo-β-cyclodextrin-Sepharose HP for the preparation of high purity daidzin from crude soybean samples was proposed in this work. The isoflavone of daidzin in sample A and B was purified under the optimum mobile phase composed of methanol/acetic acid/water=20.0/8.0/72.0 (v/v/v) at a flow-rate of 1.0 mL/min in one-step operation with a purity of 97.2% and 98.1%, a recovery of 95.3% and 96.3% respectively. The target products in isolated fraction were detected and characterized by HPLC analysis and ESI-MS spectrum. Preparative separation with sample-load of up to 2.42 mg/mL medium gave satisfactory results for daidzin with the purities over 97% and recoveries approximately 90%. Molecular docking simulations were utilized to help demonstrate the inclusion complexation between β-cyclodextrin and the isoflavones in samples through inclusion geometries and calculations of the binding energies. The prediction of the elution orders with AUTODOCK and SURFLEX-DOCK were validated by the chromatographic results.

    Topics: beta-Cyclodextrins; Chromatography, High Pressure Liquid; Glycine max; Isoflavones; Models, Statistical; Molecular Dynamics Simulation; Plant Extracts; Sepharose; Spectrometry, Mass, Electrospray Ionization

2011