cancidas has been researched along with nikkomycin* in 2 studies
2 other study(ies) available for cancidas and nikkomycin
| Article | Year |
|---|---|
Differential effects of inhibiting chitin and 1,3-{beta}-D-glucan synthesis in ras and calcineurin mutants of Aspergillus fumigatus.
Aspergillus fumigatus must be able to properly form hyphae and maintain cell wall integrity in order to establish invasive disease. Ras proteins and calcineurin each have been implicated as having roles in these processes. Here, we further delineate the roles of calcineurin and Ras activity in cell wall biosynthesis and hyphal morphology using genetic and pharmacologic tools. Strains deleted for three genes encoding proteins of these pathways, rasA (the Ras protein), cnaA (calcineurin), or crzA (the zinc finger transcription factor downstream of calcineurin), all displayed decreased cell wall 1,3-beta-d-glucan content. Echinocandin treatment further decreased the levels of 1,3-beta-d-glucan for all strains tested yet also partially corrected the hyphal growth defect of the DeltarasA strain. The inhibition of glucan synthesis caused an increase in chitin content for wild-type, dominant-active rasA, and DeltarasA strains. However, this important compensatory response was diminished in the calcineurin pathway mutants (DeltacnaA and DeltacrzA). Taken together, our data suggest that the Ras and calcineurin pathways act in parallel to regulate cell wall formation and hyphal growth. Additionally, the calcineurin pathway elements cnaA and crzA play a major role in proper chitin and glucan incorporation into the A. fumigatus cell wall. Topics: Aminoglycosides; Antifungal Agents; Aspergillus fumigatus; beta-Glucans; Calcineurin; Caspofungin; Cell Wall; Chitin; Echinocandins; Fluorescent Dyes; Fungal Proteins; Genes, Fungal; Genes, ras; Lipopeptides; Microbial Sensitivity Tests; Microscopy, Fluorescence; Mutation; Signal Transduction | 2009 |
Calcineurin inhibition or mutation enhances cell wall inhibitors against Aspergillus fumigatus.
Calcineurin mutation or inhibition enhanced the antifungal morphological effect of cell wall inhibitors caspofungin or nikkomycin Z against Aspergillus fumigatus. Quantification of 1,3-beta-d-glucan revealed decreased amounts in the calcineurin A (DeltacnaA) mutant. Calcineurin can be an excellent adjunct therapeutic target in combination with other cell wall inhibitors against A. fumigatus. Topics: Aminoglycosides; Antifungal Agents; Aspergillus fumigatus; Calcineurin; Calcineurin Inhibitors; Caspofungin; Cell Wall; Drug Synergism; Echinocandins; Enzyme Inhibitors; Gene Expression Regulation, Fungal; Humans; Lipopeptides; Microbial Sensitivity Tests; Mutation; Peptides, Cyclic; Tacrolimus | 2007 |