3-hydroxyacyl-CoA dehydratase activity

Definition

Target type: molecularfunction

Catalysis of the reaction: a 3-hydroxy-fatty acyl-CoA = a (2E)-enoyl-CoA + H2O. [RHEA:33767]

3-Hydroxyacyl-CoA dehydratase activity is a crucial step in the β-oxidation pathway, responsible for the breakdown of fatty acids to generate energy. This enzymatic activity catalyzes the dehydration of 3-hydroxyacyl-CoA to form a trans-2-enoyl-CoA. The mechanism involves the abstraction of a proton from the C-3 carbon of the 3-hydroxyacyl-CoA, followed by the removal of a hydroxyl group from the same carbon. This results in the formation of a double bond between the C-2 and C-3 carbons, generating a trans-2-enoyl-CoA molecule. The enzyme requires a specific substrate, 3-hydroxyacyl-CoA, which has a distinct structure and chemical properties. It interacts with the enzyme's active site, facilitating the dehydration reaction. The dehydration process is stereospecific, producing only the trans isomer of the enoyl-CoA product. This specificity is essential for the proper functioning of the β-oxidation pathway. The 3-hydroxyacyl-CoA dehydratase activity is an important metabolic function, providing a vital link in the catabolism of fatty acids. It plays a key role in generating energy from fatty acids, which are a major source of fuel in many organisms.'
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Proteins (1)

Compounds (1)