Page last updated: 2024-10-24

oxygen-dependent protoporphyrinogen oxidase activity

Definition

Target type: molecularfunction

Catalysis of the reaction: 3 O2 + protoporphyrinogen IX = 3 H2O2 + protoporphyrin IX. [EC:1.3.3.4, RHEA:25576]

Oxygen-dependent protoporphyrinogen oxidase (PPO) is a critical enzyme in heme biosynthesis, catalyzing the oxidation of protoporphyrinogen IX to protoporphyrin IX. This step involves the incorporation of molecular oxygen to create the final porphyrin ring structure necessary for heme production. PPO is an integral membrane protein found in mitochondria, and it requires molecular oxygen as a substrate.

The precise molecular mechanism of PPO activity is complex and involves several steps:

1. Binding: PPO binds to protoporphyrinogen IX, the substrate, and molecular oxygen.
2. Electron Transfer: PPO facilitates the transfer of electrons from protoporphyrinogen IX to molecular oxygen, converting the latter to water.
3. Oxidation: This electron transfer process leads to the oxidation of protoporphyrinogen IX, converting it into protoporphyrin IX. This conversion involves the formation of a double bond in the porphyrin ring structure.
4. Release: After the oxidation, protoporphyrin IX is released from PPO, leaving the enzyme ready to catalyze another round of the reaction.

The enzyme's active site contains several key amino acids that participate in this catalytic process. These amino acids, including tyrosine residues, are involved in binding the substrate and facilitating electron transfer. The reaction requires a specific arrangement of these residues for optimal catalytic activity.

The proper functioning of PPO is crucial for the biosynthesis of heme, a vital molecule with various roles in cellular respiration, oxygen transport, and other important processes. Mutations or deficiencies in PPO can lead to various diseases, including porphyrias, characterized by the accumulation of porphyrin precursors in the body.'
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Proteins (1)

ProteinDefinitionTaxonomy
Protoporphyrinogen oxidaseA protoporphyrinogen oxidase that is encoded in the genome of human. [PRO:DNx, UniProtKB:P50336]Homo sapiens (human)

Compounds (3)

CompoundDefinitionClassesRoles
oxadiazonoxadiazon: manufactured by the Societe Rhone-poulenc, France; structurearomatic ether
acifluorfenaromatic ether;
benzoic acids;
C-nitro compound;
monocarboxylic acid;
organochlorine compound;
organofluorine compound
agrochemical;
EC 1.3.3.4 (protoporphyrinogen oxidase) inhibitor;
herbicide
n-(2,4-dichloro-5-(4-(difluoromethyl)-4,5-dihydro-3-methyl-5-oxo-1h-1,2,4-triazol-1-yl)phenyl)methanesulfonamideN-(2,4-dichloro-5-(4-(difluoromethyl)-4,5-dihydro-3-methyl-5-oxo-1H-1,2,4-triazol-1-yl)phenyl)methanesulfonamide: FP 846 is purified form of sulfentrazone; protoporphyrinogen oxidase inhibitor

sulfentrazone : A member of the class of triazoles that is 5-oxo-1,2,4-triazole which is substituted at positions 1, 3, and 4 by 2,4-dichloro-5-[(methylsulfonyl)amino]phenyl, methyl, and difluoromethyl groups, respectively. A protoporphyrinogen oxidase inhibitor, it is used as a herbicide to control broad-leaved weeds in soya and tobacco crops. Not approved for use within the European Union.
dichlorobenzene;
organofluorine compound;
sulfonamide;
triazoles
agrochemical;
EC 1.3.3.4 (protoporphyrinogen oxidase) inhibitor;
herbicide